LOCUS       V004278                 6069 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004278
VERSION     V004278
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Park HH, Lim WK, Shin HJ.
  TITLE     In vitro binding of purified NahR regulatory protein with promoter
            Psal
  JOURNAL   Biochim. Biophys. Acta 1725 (2), 247-255 (2005)
   PUBMED   15978733
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Park HH, Lim WK, Shin HJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2002) Dongseo University, San 69-1, Jurye -2-dong,
            Sasang-gu, Busan 617-716, Korea
REFERENCE   3  (bases 1 to 6069)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6069)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim.
            Biophys. Acta"; date: "2005"; volume: "1725"; issue: "2"; pages:
            "247-255"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-MAY-2002) Dongseo University, San 69-1, Jurye -2-dong,
            Sasang-gu, Busan 617-716, Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(236..1135)
                     /gene="nahR"
                     /label="HTH-type transcriptional activator NahR"
                     /note="HTH-type transcriptional activator NahR from
                     Pseudomonas putida. Accession#: P10183"
     regulatory      complement(1136..1225)
                     /label="Pr promoter"
                     /note="Pr promoter"
                     /regulatory_class="promoter"
     regulatory      1214..1282
                     /label="Psal promoter"
                     /note="Psal promoter"
                     /regulatory_class="promoter"
     CDS             1339..2988
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(3032..3153)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3572..4160)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4334..5191)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5192..5296)
                     /label="AmpR promoter"
     rep_origin      5323..5778
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5909..5957
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    5971..6062
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tctcaattct ctctatcctg cgatcccgcg aagaaccaaa aaagctcgac
       61 agagggcgcg gtcattttag gtcgggcgga tcggcgccgc cggctcggct ggtgtgccgc
      121 acagcaccgc ctacgtgagc tgccagttga tgaacttccc ccgttgccag ctagggcgca
      181 agcgggctgt ataagatcac tgcccatcac attgatcggc tcggattttt tctcaatccg
      241 taaacaggtc aaacatcagt tgccgcaacc aaatattggc taggtccttg tggtacttcg
      301 catgccagaa catgttgatg gctatttcag gcaagacgac tgggtgcggc aaggcgctta
      361 ggccgaaggg ctccacgcag cagtcggcta aacgtatcgg cacagtggcg agcagatcgg
      421 tgcgctggag gatgtggcca acggcggcga agtgcggcac ttccagacgg atgtcgcgcc
      481 ggatgccgac ccgtgtcatg tacgtgtcca cctcgccgtg gccggtgcca gcggcgatga
      541 cacgcacgtg gccgtaggaa cagaagcgct ccagagtcag gggttcgcgg gtgactggat
      601 ggtccttgcg acataggcac acgtagtgat tctggagcag ccggcgctga aagaagccag
      661 tttgcagatt gggaagcagg cccacggcca agtccacggt tccgttctgc aaggcctgca
      721 tcaggctcat cgaactgtcg cgcaccgtac tgatcacgca attgggggcc tggtgagcca
      781 gcacatccat cagccgcggc atgaagtaga tctcgccaat gtcggtcatg gccagggtga
      841 aggtacgctc gctggtcagc ggatcgaagc tttcatggtg ctgtagggcg ttgcgcagtg
      901 cgtgcatggc cgaagtgacg ggctcggcca gatgcgcggc atagggtgtg ggttccattc
      961 cctgatgtgt gcgcacgaag agtgggtcct gtagcgaggt gcgcaggcgt ttcagcgcat
     1021 tgctcacggc aggctgggtc aggcccaggt tctccgcagt gatagagacg cgtctgtcga
     1081 ccagcaactg gttgaacacc accagcaggt ttaaatccag gtcacgcagt tccatggggc
     1141 ctcgcttggg ttattgctgg tgcccggccg ggcgcaatat tcatgttgat gatttattat
     1201 atatcgagtg gtgtatttat caatattgtt tgctccgtta tcgttattaa caagtcatca
     1261 ataaagccat cacgagtaca gcctcgagat ctgcgatcta agtaagcttg gcattccggt
     1321 actgttggta aagccaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc
     1381 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
     1441 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct
     1501 gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
     1561 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
     1621 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
     1681 ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa
     1741 aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct
     1801 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
     1861 ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg
     1921 atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact
     1981 gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
     2041 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
     2101 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg
     2161 aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc
     2221 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
     2281 ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca
     2341 ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
     2401 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
     2461 gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt
     2521 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
     2581 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc
     2641 gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg
     2701 gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac
     2761 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
     2821 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
     2881 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
     2941 atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagagt
     3001 cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     3061 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     3121 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     3181 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     3241 ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     3301 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     3361 gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc
     3421 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3481 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4081 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4141 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4201 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4261 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4321 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4381 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4441 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4501 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4561 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4621 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4681 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4741 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4801 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4861 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4921 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4981 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5041 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5101 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5161 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5221 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5281 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     5341 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5401 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5461 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5521 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     5581 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     5641 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     5701 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     5761 tattaacgct tacaatttgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     5821 cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc atgataagta agtaatatta
     5881 aggtacggga ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg
     5941 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa
     6001 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     6061 ctatcgata
//
