pNOV108 vector (V004289)

LOCUS       V004289                 8522 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004289
VERSION     V004289
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Richie DL, Takeoka KT, Bojkovic J, Metzger LE IV, Rath CM, Sawyer
            WS, Wei JR, Dean CR.
  TITLE     Toxic Accumulation of LPS Pathway Intermediates Underlies the
            Requirement of LpxH for Growth of Acinetobacter baumannii ATCC 19606
  JOURNAL   PLoS ONE 11 (8), E0160918 (2016)
   PUBMED   27526195
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2016) Infectious Diseases, Bacteriology, Novartis
            Institutes for Biomedical Research, 5300 Chiron way, Emeryville, CA
            94608, USA
REFERENCE   3  (bases 1 to 8522)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8522)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2016"; volume: "11"; issue: "8"; pages: "E0160918"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-JUL-2016) Infectious Diseases, Bacteriology, Novartis Institutes
            for Biomedical Research, 5300 Chiron way, Emeryville, CA 94608, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..1337
                     /label="derived from Acinetobacter ATCC23237"
                     /note="derived from Acinetobacter ATCC23237"
     CDS             1590..2669
                     /label="lacI"
                     /note="lac repressor"
     CDS             complement(2784..5417)
                     /gene="alaS"
                     /label="Alanine--tRNA ligase"
                     /note="Alanine--tRNA ligase from Acinetobacter baumannii
                     (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 /
                     5377). Accession#: A3M3W2"
     promoter        5687..5778
                     /label="AmpR promoter"
     CDS             5779..6636
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6692..7147
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      7258..7846
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(8032..8172)
                     /label="bom"
                     /note="basis of mobility region from pBR322"