pNOV108 vector (V004289)

MSDS

Basic Vector Information

Vector Name:: pNOV108

Antibiotic Resistance:: Ampicillin

Length:: 8522 bp

Type:: Expresssion vector

Replication origin:: ori

Source/Author:: Richie DL, Takeoka KT, Bojkovic J, Metzger LE IV, Rath CM, Sawyer WS, Wei JR, Dean CR.

pNOV108 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNOV108 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33452        8522 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expresssion vector pNOV108, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Richie DL, Takeoka KT, Bojkovic J, Metzger LE IV, Rath CM, Sawyer 
            WS, Wei JR, Dean CR.
  TITLE     Toxic Accumulation of LPS Pathway Intermediates Underlies the 
            Requirement of LpxH for Growth of Acinetobacter baumannii ATCC 19606
  JOURNAL   PLoS ONE 11 (8), E0160918 (2016)
  PUBMED    27526195
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2016) Infectious Diseases, Bacteriology, Novartis 
            Institutes for Biomedical Research, 5300 Chiron way, Emeryville, CA 
            94608, USA
REFERENCE   3  (bases 1 to 8522)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8522)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "8"; pages: "E0160918"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2016) Infectious Diseases, Bacteriology, Novartis Institutes
            for Biomedical Research, 5300 Chiron way, Emeryville, CA 94608, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..1337
                     /label=derived from Acinetobacter ATCC23237
                     /note="derived from Acinetobacter ATCC23237"
     CDS             1590..2669
                     /label=lacI
                     /note="lac repressor"
     CDS             complement(2784..5417)
                     /gene="alaS"
                     /label=alaS
                     /note="Alanine--tRNA ligase from Acinetobacter baumannii
                     (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 
                     5377). Accession#: A3M3W2"
     promoter        5687..5778
                     /label=AmpR promoter
     CDS             5779..6636
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6692..7147
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      7258..7846
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8032..8172)
                     /label=bom
                     /note="basis of mobility region from pBR322"

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