LOCUS       V004289                 8522 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004289
VERSION     V004289
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Richie DL, Takeoka KT, Bojkovic J, Metzger LE IV, Rath CM, Sawyer
            WS, Wei JR, Dean CR.
  TITLE     Toxic Accumulation of LPS Pathway Intermediates Underlies the
            Requirement of LpxH for Growth of Acinetobacter baumannii ATCC 19606
  JOURNAL   PLoS ONE 11 (8), E0160918 (2016)
   PUBMED   27526195
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2016) Infectious Diseases, Bacteriology, Novartis
            Institutes for Biomedical Research, 5300 Chiron way, Emeryville, CA
            94608, USA
REFERENCE   3  (bases 1 to 8522)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8522)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2016"; volume: "11"; issue: "8"; pages: "E0160918"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-JUL-2016) Infectious Diseases, Bacteriology, Novartis Institutes
            for Biomedical Research, 5300 Chiron way, Emeryville, CA 94608, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..1337
                     /label="derived from Acinetobacter ATCC23237"
                     /note="derived from Acinetobacter ATCC23237"
     CDS             1590..2669
                     /label="lacI"
                     /note="lac repressor"
     CDS             complement(2784..5417)
                     /gene="alaS"
                     /label="Alanine--tRNA ligase"
                     /note="Alanine--tRNA ligase from Acinetobacter baumannii
                     (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 /
                     5377). Accession#: A3M3W2"
     promoter        5687..5778
                     /label="AmpR promoter"
     CDS             5779..6636
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6692..7147
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      7258..7846
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(8032..8172)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 atcgatcaac cgctctgtag gcttgaagaa cgcaacccta tagcagctat tgaaattgat
       61 gatttaattg aagaaaagac agatttactt gttgataatc gactgatggg gcgcacaggc
      121 agacagaaag atactaggga gttagtgata catccgcatt atgtggttgt atatgacatc
      181 actgatataa tacggatact cagagtgcta cacacatcgc atgagtggtc atgacttact
      241 catgtacttt ggattattta gtgttataaa atcctgattt ataaattttt ttttgttaaa
      301 aaagataaaa gccccttgca attgcttggg gctttaccgt aatttatggg gtacagatct
      361 tcgatactga catatcggca atcgaaagca ttaaggtttg acgaccgcta atgatttcac
      421 cacaggggct taatgtacct gtcttaaatt ctaaggtttt aactcgcttt gtcaagcata
      481 gaccccaaaa atttagccaa tgtctgtaac tcaatctgtc catgtgtggg tgatgaggta
      541 cagtgacgct agcacacatc ggaaaaacgc tattactagg ggaactgaac agagtagcgg
      601 acgcaatgag tagtcattta attggcggtt atgagcgtgt tcaggcggtg ctatcaatcg
      661 taatcataac agtggcagct tgatacagtg atgtcatccc tgatgcgaaa gcgaccgacc
      721 gacggtacat cgaatgggaa tactttaggg tgatttttaa gaatcgctct agggtgagta
      781 tttcccattc agctctgctc cctccctctg gtactttaat caaaagcact actaaacata
      841 tgtttttaaa taaaaaatat tgatatagag ataatattag taagaataat taaacaattg
      901 aatatagata aatcattgtt aaataaagat taattattaa aatgaatgta tacttatata
      961 taaatcaatg atttaaaata tttgataaag aaaacttttc aaaaaaaata taattgagat
     1021 tgtgtcattt cggtcaattc ttaatatgtt ccacgcaagt tttagctatg gtgctaaaca
     1081 gaaatttgct gaaaaagaac ttttcactga actggttaaa atgtaagcag cctgagagcc
     1141 gccaaaaatt ttaaaaacaa accgccttaa tcatcttcaa aaaatacctc taaaacctca
     1201 ccatttgcgt tttaagaccc atatttcatc ctgcccttat gttcccatgc tgatagctat
     1261 aaagtgtctg taatcgcttc ctatgacgtt ctaggctgtt gataactttt ggaacaacgc
     1321 cgaaacaggc tcaccctgat gcataatgtg cctgtcaaat ggacgaagca gggattctgc
     1381 aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaag acagcaagcg
     1441 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     1501 ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga
     1561 caggatgagg atcgtttcgc atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
     1621 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
     1681 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
     1741 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
     1801 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
     1861 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
     1921 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
     1981 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
     2041 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
     2101 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
     2161 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
     2221 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
     2281 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
     2341 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
     2401 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
     2461 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     2521 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     2581 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2641 gacaggtttc ccgactggaa agcgggcagt gataccgagc tcgaattgac ataagcctgg
     2701 aacgattaat gccatagttg ttccatataa gccatgaccc tgagaggctt gtcagggtca
     2761 gttacacaaa aagtgaagtc ttatttttgt tcaagccaag caggtaatgc tgccataact
     2821 tgtccaaact tctcgttaag tggcgcgcca ccttgtgcta agtcaggttt accaccacct
     2881 ttaccaccta actcggtcgc taaatgtttg atgatgtcac ctgcttttag atttgcagtg
     2941 tattgcgacg cgactgatgc aatcaggctg actttgtcac cctctacacc tgctaacaca
     3001 atcactgcgt tctctaattt tgattttaca ctgtcatgta agttgcgaag cgctttggca
     3061 tcaacaccct gaacagtagt aataagcgtt tgacgaccag caatagtttg aacttggtca
     3121 atcaaatcag ccgcttggaa gtttgccaat ttttggttga gttgttcaat ctgcttttgt
     3181 aatgcagaaa ccaattctac attggcttgt acacgctcaa ccgtttggtc tttctgtgct
     3241 tttaataagc tattaatgtg ctggatatca tgatcagctt tttgtactac ttctaatgct
     3301 ttagtaccag tcactgcttc aatacgacgt acgcctgcag ctacgccacc ttcagaagtg
     3361 attttgaata aaccaatatc acctgtacgt tttacgtgaa taccaccaca aagttcgatt
     3421 gagaagtttt tctcgtcaat gactgagccc atagagagta cacgaacttc atcaccgtat
     3481 ttttcaccga acaacatcat tgcgcctttt gctttcgcag tttcaatgtc gagtagttca
     3541 gttgttacag cagtatttgc aatgatttca gcatttacga gacgttcaac ttgttgtagt
     3601 tgttcaaatg acacaggttg gtcatttgca aagtcaaaac gtaagatatc acttgcaacc
     3661 aaagagcctt tttgttgtac atgtgaacct aaaatttgac gaagggcagc atgcaacaag
     3721 tgagttgctg agtggttacg cgcagtagct tcacgaatgt ctgctttaac aatcgcttct
     3781 acattttgac tggttttaag gttaccaaca gtcacaatac cttgatgaac aaaggcgcca
     3841 ccagactttt tcgtatcctg aacttcaaaa atacctgttt cgtttttgaa gataccagta
     3901 tcaccaatct gaccaccgct ttcagcatag aaaggagttt ggtttaaaac gataagtgct
     3961 tcgtcgcctt cagaaacttc atcaacttgt acgccatctt tatagatcgc tacaatctgg
     4021 ccttgaccgg ttgtgttggt ataaccgtcg aattgagttt cgccttcaac ttttacaatg
     4081 ttgttatagt caacagcaaa tttaccagca tcacgcgcac gttgacgctg tgcagccatt
     4141 tcaacttcaa aacctgcttc atcaataata aagccacgtt cacgtgcaat atcagcagtt
     4201 aaatctgttg ggaaaccgta agtgtcgtaa agtttaaata cagtcgcacc cggaatagtt
     4261 ttatctttta actgagcaag ttcaccttca agaagtttta aaccttgctc taaggtttta
     4321 gcaaactgtt cttcttcacg aattaaggtt gcttcaatga cttcacggcg agcttctaac
     4381 tcaggataag cctgtcccat cacttcaatt aatggttgca acattttgta gaagaacgta
     4441 cctgttgcgc caagcttgtt accgtgacgg acagcacggc ggataatacg acgtaataca
     4501 taaccacgtc cttcattact tgggtttaca ccatcagcaa ttaagaagca gcaagaacgt
     4561 gcatggtctg caactacacg taaagaaggc tgaccttcat cttcaatacc aatgatgttt
     4621 gcagcagctt ttaataagtg ttggaacagg tcaatgtcat agtttgaatt aacatgttgc
     4681 aatacggcag aaatacgctc taagcccata ccggtatcta cagatggagc agggagtggg
     4741 tgaagtacgc catcagcagt acggttaaac tgcatgaaaa cgttgttcca gatttcaatg
     4801 aaacggtcac cgtcttcttc aggtgaacca ggtaaaccac cccaaatgtg ttcaccgtgg
     4861 tcgaagaaaa tttctgaaca tggaccgcaa ggacctgtat cacccattgc ccagaagtta
     4921 tctgatgcgt atttttcgcc tttgttatca ccaatacgga taatacgttc tggtgctagg
     4981 ccgatttctt tattccagat gtcataagct tcgtcatcag tatggtaaac cgtgacataa
     5041 agtttatctt ttggtaaagc aagccattgt tcgctagtta aaaattccca tgcgaacttg
     5101 agagcatcac gtttgaaata gtcaccaaat gagaagttac ccaacatttc aaagaatgta
     5161 tggtgacgag ctgtataacc aacattatct aaatcgttat gtttaccacc tgcacgtaca
     5221 catttttgtg atgttgttgc acgtacataa tcacgttttt caagacctaa aaaacagtct
     5281 ttaaactggt tcatcccagc atttgtaaat aataaagttg ggtcgttggc aggaacaaga
     5341 gaactcgatg caacacgtgt atgcccttgc gtttcaaagt agcgcaaaaa tgcttcacga
     5401 atttcagcag atgtcattaa acgagtactc acaaccaagt cactccatat tttcgaattt
     5461 ggctaaaagc atcaccacaa gtttatttgt tcacaaaact cgcatgtgat acggggctta
     5521 tttttaaaaa acgctaaatt gtaacggaaa aaccccaccg aaccaacgat tttagaggga
     5581 atatcttaca aaactgtgat ttggctaaaa atctatattc aattagccat gacgtgtttt
     5641 actatatgcc ttctttcgct tctcgatttg tagataagga taggtctttg tttatttttc
     5701 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5761 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5821 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5881 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5941 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6001 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     6061 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6121 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6181 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6241 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6301 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     6361 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6421 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6481 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6541 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6601 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6661 tatatacttt agattgactt tagattgatt tacgcgccct gtagcggcgc attaagcgcg
     6721 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6781 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6841 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6901 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6961 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaacttg aacaacactc
     7021 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7081 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     7141 acaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7201 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7261 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7321 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7381 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7441 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7501 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7561 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7621 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7681 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7741 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7801 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7861 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7921 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7981 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     8041 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     8101 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     8161 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     8221 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     8281 ttttcaccgt catcaccgaa acgcgcgagg cagcaaggag atggcgccca acagtccccc
     8341 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     8401 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
     8461 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta
     8521 tc
//
