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pNOV044 vector (V004293)

Basic Vector Information

Vector Name:
pNOV044
Antibiotic Resistance:
Gentamycin
Length:
10383 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Wei J-R.
Promoter:
Pc

pNOV044 vector Vector Map

pNOV04410383 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000Pc promoterGmRf1 orioribom

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNOV044 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33432       10383 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNOV044, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Wei J-R.
  TITLE     LpxK is essential for growth of Acinetobacter baumannii ATCC 19606: 
            relationship to toxic accumulation of lipid A pathway intermediates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2017) Infectious Diseases, Bacteriology, Novartis 
            Institutes for Biomedical Research, 5300 Chiron Way, Emeryville, CA 
            94608, USA
REFERENCE   3  (bases 1 to 10383)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10383)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2017) Infectious Diseases, Bacteriology, Novartis Institutes
            for Biomedical Research, 5300 Chiron Way, Emeryville, CA 94608, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..1337
                     /regulatory_class="replication_regulatory_region"
     regulatory      1430..1629
                     /regulatory_class="promoter"
     promoter        7781..7809
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             7998..8528
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     rep_origin      8553..9008
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      9119..9707
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9893..10033)
                     /label=bom
                     /note="basis of mobility region from pBR322"

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