LOCUS       40924_33432       10383 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNOV044, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Wei J-R.
  TITLE     LpxK is essential for growth of Acinetobacter baumannii ATCC 19606: 
            relationship to toxic accumulation of lipid A pathway intermediates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2017) Infectious Diseases, Bacteriology, Novartis 
            Institutes for Biomedical Research, 5300 Chiron Way, Emeryville, CA 
            94608, USA
REFERENCE   3  (bases 1 to 10383)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10383)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2017) Infectious Diseases, Bacteriology, Novartis Institutes
            for Biomedical Research, 5300 Chiron Way, Emeryville, CA 94608, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..1337
                     /regulatory_class="replication_regulatory_region"
     regulatory      1430..1629
                     /regulatory_class="promoter"
     promoter        7781..7809
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             7998..8528
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     rep_origin      8553..9008
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      9119..9707
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9893..10033)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 atcgatcaac cgctctgtag gcttgaagaa cgcaacccta tagcagctat tgaaattgat
       61 gatttaattg aagaaaagac agatttactt gttgataatc gactgatggg gcgcacaggc
      121 agacagaaag atactaggga gttagtgata catccgcatt atgtggttgt atatgacatc
      181 actgatataa tacggatact cagagtgcta cacacatcgc atgagtggtc atgacttact
      241 catgtacttt ggattattta gtgttataaa atcctgattt ataaattttt ttttgttaaa
      301 aaagataaaa gccccttgca attgcttggg gctttaccgt aatttatggg gtacagatct
      361 tcgatactga catatcggca atcgaaagca ttaaggtttg acgaccgcta atgatttcac
      421 cacaggggct taatgtacct gtcttaaatt ctaaggtttt aactcgcttt gtcaagcata
      481 gaccccaaaa atttagccaa tgtctgtaac tcaatctgtc catgtgtggg tgatgaggta
      541 cagtgacgct agcacacatc ggaaaaacgc tattactagg ggaactgaac agagtagcgg
      601 acgcaatgag tagtcattta attggcggtt atgagcgtgt tcaggcggtg ctatcaatcg
      661 taatcataac agtggcagct tgatacagtg atgtcatccc tgatgcgaaa gcgaccgacc
      721 gacggtacat cgaatgggaa tactttaggg tgatttttaa gaatcgctct agggtgagta
      781 tttcccattc agctctgctc cctccctctg gtactttaat caaaagcact actaaacata
      841 tgtttttaaa taaaaaatat tgatatagag ataatattag taagaataat taaacaattg
      901 aatatagata aatcattgtt aaataaagat taattattaa aatgaatgta tacttatata
      961 taaatcaatg atttaaaata tttgataaag aaaacttttc aaaaaaaata taattgagat
     1021 tgtgtcattt cggtcaattc ttaatatgtt ccacgcaagt tttagctatg gtgctaaaca
     1081 gaaatttgct gaaaaagaac ttttcactga actggttaaa atgtaagcag cctgagagcc
     1141 gccaaaaatt ttaaaaacaa accgccttaa tcatcttcaa aaaatacctc taaaacctca
     1201 ccatttgcgt tttaagaccc atatttcatc ctgcccttat gttcccatgc tgatagctat
     1261 aaagtgtctg taatcgcttc ctatgacgtt ctaggctgtt gataactttt ggaacaacgc
     1321 cgaaacaggc tcaccctgat gcataatgtg cctgtcaaat ggacgaagca gggattctgc
     1381 aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaaa gacattgagc
     1441 aactaactca gaaattatca gaacgttttg gtgccaatgt taaaattgat cataaccaga
     1501 aaggtaaagg taagcttgtt attcactatc attcactcga tgagttagat ggtattttga
     1561 atatttgttt accagattaa ttctaagtta ggtcatatat ttctaattta tattcttggg
     1621 gaagaaaata tgaaacatat cgtcattcca gcacgcttct caagttcgcg tttgccaggt
     1681 aagccattgt tacttattca tgatcgccct atgattttac gagtggtgga tcaagcaaaa
     1741 aaagttgaag gtttcgatga cctatgtgta gcaaccgacg atgaacgtat cgctgaaata
     1801 tgtcgtgcag agggcgttga tgttgtttta acaagtgcag accatccatc aggaacagat
     1861 cgtttaagtg aagttgctcg tattaaagga tgggatgctg atgacattat tgtaaatgta
     1921 caaggcgatg agccattact gccagcccag cttgttcagc aagttgcaaa gcttttggtt
     1981 gataagccaa actgttcaat gtctacatta tgtgagccta ttcatgcgtt ggatgaattt
     2041 cagcgtgata gtatcgtaaa agtagtcatg tctaaacaaa atgaagcatt atattttagt
     2101 cgtgcaacta ttccttatga tcgagatagt gctaaacaag ctgaaccaac tttacatagc
     2161 caagcatttc gtcatttagg tttatatgct tatcgtgtga gcttattaca agaatatgtt
     2221 acatgggaaa tgggtaaact tgaaaagctc gagagtcttg agcaattgcg tgttttagaa
     2281 aatggacacc gtattgctat tgctgtagca gaagcgaatt taccaccagg tgttgacact
     2341 caagtagatc ttgatcgttt aaataatatg cctgttgagt cttttgagta agcgttaatt
     2401 taatgaatca gaatgtgact tctaaggttt atccgtggca acagacaatg tgggaaacgt
     2461 taacaacacg ttttcctaat attgggcatg gcttattatt ttatggtaaa caaggttgtg
     2521 gtaaacatgc ttttgctaag catttcttag cgtgggtact ttgtttaaat aaacaacctc
     2581 aaggtgcttg tggtgaatgt agtagctgtc aatggttaaa atcagatacg catccaaact
     2641 atgttcatat cacgacagat gaagaaaata aaaaacagaa tgcaaaaatt aaaatagaaa
     2701 aaattcgaga tttattgcca tttgtacaac aaactggtga gggatggcgt gttgttgtta
     2761 ttgagccagc tgaagcttta aatcttgcgt catcaaatgc gctgctaaaa accttagaag
     2821 agccaggtga acgcgtcgtt ttaatcttgt tggccgatca ttatttgaaa ttaccggcaa
     2881 ccattcgaag tcgcttacag cattttgctt tagaccgtat ttcatataca gaagccacat
     2941 cttatctaaa tgaacattta gcggaaacag catctgtacc acctgaatta ttgctaggcc
     3001 tttctaatga tatgcctttg caagcagttg aaattgcgac tagtgattgg tttgctaaaa
     3061 gacagtcttt tttagaagat tggactaaag ttgtaaatca aaaaaatatg cctttatttt
     3121 attcggctaa atggcaaaaa gaactcaact ttagtgattt tatgatgctt ctcgaatact
     3181 tgttagggga tttaatttgt gtcaagctca atcaaccaca gaaaaatata gatttacagt
     3241 tcgatcaatt ggcgcaaaac tataatttag aaagtttatt cagtctttat gaggaattac
     3301 agcaatctaa aagattggtt gaacagaatg tacaaacaca actcatcata gatcaacttt
     3361 ttataaaatt aatgaacgtt taaggggaaa actaatgcaa ccacaaatgg gtggaattat
     3421 tcaggtcaat attccagata gagcaacatt acaagcaagt tatatgggat atgtacaagg
     3481 tgggggcttg tttgtaccat cgaagcaaaa agtgaagatg ggtcaggaaa tttttatttt
     3541 agctacgtta ccagaacaat cacaaaaaat tcctttaact ggtaaagtag tttggatttc
     3601 acataagcaa agtggattta aaccgcaagg tttcgcaatt caattgagtg gtgataaagg
     3661 aatttactat aaagctgaag ctgagcggat tttagctggt agtatgtctt tagatcgccc
     3721 aagttatacc atgtaagcag ataggaaaga acagtgtttg ttgataccca ttgccattta
     3781 acaatgttag atttaacgcc ttataacggt gatttagatc tcgcacttgc tgctgcgcga
     3841 gcagaaggtg tttctaagtt tatggcaatt tcagttgacc ttgatgatca tattgcttta
     3901 gctgaaatcg caaagcgtca tgaagatgtg gggtatacgg taggcgtaca tccgtgtgag
     3961 gatgttgata ctatggctcg agcaacgact gagtatttga ctgaattggc tcaatcagaa
     4021 aaggtatggg ctttaggtga aacaggttta gactattacc acagcactga ctttattaac
     4081 gagcaaaaag attgttttgc tcgtcatatt caagcctcta aaaatgttaa aaaaccagta
     4141 gttgttcata cacgttcagc aaaacatgat acggtggata ttattcgagc tgaacaatct
     4201 acacatggta ttttgcactg ttttactgag gactgggaaa cagcaaaagc tgtattggac
     4261 tgtggttact atatttcctt ttcaggaatt gtttcattta aaaatgctca agatctacga
     4321 gatgtagcca agcaagttcc tttagatcgg cttttgattg agacagatag cccttatttg
     4381 gctccagtac catatcgtgg taaaacgaat gaaccaaaat atgtacctta tgtagcgaaa
     4441 gcactaagtg aggtatatga taagccggtt gaggaaattg caagaatcac ctcgcagaat
     4501 tttgaaaatt tacttaaagc gatttaactt atagaaacta tataaagaga gtaattgcgt
     4561 gaagatggac cgtcaagctc agttccgagc aagagaagca ttaatatttc aagttgcgga
     4621 acagttgttg ttagaaaatg gcgaagccgg aatgacactt gatgtgctgg ctgctgagct
     4681 tgatttggca aaaggtactt tgtacaagca ctttcaaagt aaagatgaat tgtacatgct
     4741 gttaattatt cgtaatgagc gcatgttact ggaaatggtt caagatactg aaaaggcttt
     4801 cccagaacat ttagctttct ttatgttgca tcatttgcac catccagaaa gaacagtgct
     4861 gtttcaccaa attgaagaaa agctttcaat tactgcccaa ggtgtgcacc atctttttaa
     4921 tgaactttac caagtgagaa agcagcgctt acgtattatt attcgtatga ctgatcatta
     4981 tttagagtca atccagagtc atatgactac acgagattac ttagcttcaa tctggtcact
     5041 cactcatgga gctgctgcaa ttctgaattc aagcttttat caacgttatt tgggttcaag
     5101 agatacttta cgagtagcct atattgacca ggcattagcc ttacccaaac aagcagttga
     5161 gcagtacgct taataggtcg atatgaaatc gaatctacca tttcaatcac aaaaaggctt
     5221 taccctcata gaagtgatgg tagtcattgt gattatgaca attatgactt ctctggttgt
     5281 attaaatata ggtggtgtcg accaaaaaaa agcaatgcag gcgagagagt tatttttact
     5341 cgatttgcag aaaattaata aagaatcgct cgatcaatca cgtgtattgg ctttagaaac
     5401 tcatggtgag agagatgtct caccattttc ttatgaactt tatgaatatc acgatcaaag
     5461 cactttacaa gtacaagata taaaaaaccg ttggcagaaa tatactgagt ttaaaacacg
     5521 gcaacttcct gcccatgttt ctttttcagt acaacccttg gatgaccaga attattctaa
     5581 agctaaaaat acagatttga ttggaggaca aacgcctcaa ctcatctggt ttggcaatgg
     5641 agaagctaaa accgttaaaa tacaatttta tttagaacaa aaaccgattg gttcggaaat
     5701 acaaatagat cacttgggta aaataaatga aatctaaagg ctttaccctc ttagaagtta
     5761 tggttgcttt ggcaatcttt gcagtcgcag ctgtcgcttt aactaaagtg gcaatgcagt
     5821 acacgcagtc cacttcaaat gcaattttgc gaaccaaagc tcaatttgta gcaatgaatg
     5881 aagttgctat gatggagatt aatcaagaat ggttgcaagg aacccagagt aagcaagtaa
     5941 cttcccaagg tgaaacttgg caaattgata agtcagctca atctactatt agcccgaatg
     6001 ttcaaaaaat tgatttacaa gtgagtttgt atgatccgga taagggaaaa gtacaaaatg
     6061 gcattacaca tatggtcttt tttaattatc cagtgaaagc aaaataatga taaaaaataa
     6121 atatattcac atccgaagct ttgctgctcg ccttgcagca cgatcgagca gtgctcgatt
     6181 aactcgtgcc tcaggattta ctttggttga attgttagtc gcgatcgcta tctttgcggt
     6241 tttatcttta ttggggtgga aaattttcga ttacttgctc aaagttcgtg atcgtaatgc
     6301 cgaacatgaa gtgcatttat ttgaattaca agatgcttat caacaaattc ttcgtgatac
     6361 tttgcagatt atccctttat ccgcaaacca aggtgggcaa cttcatccag ctttagaaat
     6421 cgataatcaa attttacgtt ttagtaaagc aggcgtaacg gatcctttaa aacaaggctt
     6481 atcaccattt gaacgaattg aatatcgtta tgatgcagat caaaaaaaac tatatcgtct
     6541 taaatataca aatttaaata cttcgaacag agagcaacct ttatcgagta ctttgttaag
     6601 tcaagtcgac caatatcaga ttatggtttt gactccgcaa gaagtcacaa aatggcccga
     6661 ggttaatatt gatccaacga aacctaatga gttaaaaaaa ctaccgaaag ggataaaaat
     6721 ccaacttaca gtcgctggtg taaattatga atggatttat agcttaaatc agggtgactt
     6781 atcgctttca caggaaggta attcataatg gccgttagtt acaaacacca acagggtgtc
     6841 gctttactga caatacttat tatggttgcc ttggctacaa ttttagcggc ttctattgcc
     6901 aagcatcaaa ataatactat ggaaaataca gcctacttaa tacgccaaaa tcaatctttg
     6961 ctttatgcaa aaagtgcaga agcctttttt tcagaattat taattcaaga tgcaaataat
     7021 gcaggtggag tagatcattt aaaagagaca tgggcacaac ctatgcctcc gtttcctatt
     7081 gaagatggtt tggtatccgg acgtttgctt gatgagtctg gtaaatttaa tttaaataat
     7141 ctaactaatg gggaaggcat tgtaaatgaa gatgctaaaa gttggtttga acgtttattg
     7201 gtacgtgtgg gattaccagc cgaattaagc caagctgtga ttgattggca agatcgaaat
     7261 gatgagccaa ctgggccaat gggggcagaa agtagttatt atgaaggttt ggatctaggt
     7321 tacatggctg cgaatactaa attccaccgt attgaagagt taaagctagt gagaggcttt
     7381 gatggaaaaa aatttgatct aattgctcca tatatttctg ctttaccaga aaattcgaag
     7441 ttaaatatta atactgcttc gccattggtt ttagcaagta tggatgaaaa attagatata
     7501 ggagctattg aaaaagaatt gcaaacacgt caacaaaatt taaagttctt ccaaaatata
     7561 gatgaattat ggcaattgaa tgctttttca gcggttgata gtcaaaagaa aaatcaagta
     7621 aatagcctta ttgatgttaa atccagcttc tttcaagctc agattgaagt tgttctaaat
     7681 aataggaagc gacagtttac tagtgcttta atgagaaaag ataaacaagt atatgtttat
     7741 tcaagaagca tggctccatt taactaatac cgagctcgaa ttgacataag cctgttcggt
     7801 tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     7861 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgt
     7921 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     7981 gttatggagc agcaacgatg ttacgcagca gcaacgatgt tacgcagcag ggcagtcgcc
     8041 ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg cacatgtagg ctcggccctg
     8101 accaagtcaa atccatgcgg gctgctcttg atcttttcgg tcgtgagttc ggagacgtag
     8161 ccacctactc ccaacatcag ccggactccg attacctcgg gaacttgctc cgtagtaaga
     8221 cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc gcggcttacg
     8281 ttctgcccag gtttgagcag ccgcgtagtg agatctatat ctatgatctc gcagtctccg
     8341 gcgagcaccg gaggcagggc attgccaccg cgctcatcaa tctcctcaag catgaggcca
     8401 acgcgcttgg tgcttatgtg atctacgtgc aagcagatta cggtgacgat cccgcagtgg
     8461 ctctctatac aaagttgggc atacgggaag aagtgatgca ctttgatatc gacccaagta
     8521 ccgccaccta acaattcgct ttagattgat ttacgcgccc tgtagcggcg cattaagcgc
     8581 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8641 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8701 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8761 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8821 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact
     8881 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8941 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     9001 tacaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9061 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9121 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9181 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9241 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     9301 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9361 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9421 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9481 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9541 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9601 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9661 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9721 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9781 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9841 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     9901 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     9961 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
    10021 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    10081 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    10141 gttttcaccg tcatcaccga aacgcgcgag gcagcaagga gatggcgccc aacagtcccc
    10201 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
    10261 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
    10321 cgccggtgat gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt
    10381 atc
//
