Basic Vector Information
- Vector Name:
- pNOV016
- Antibiotic Resistance:
- Gentamycin
- Length:
- 5845 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Wei J-R.
pNOV016 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNOV016 vector Sequence
LOCUS 40924_33422 5845 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNOV016, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5845)
AUTHORS Wei J-R.
TITLE Direct Submission
JOURNAL Submitted (15-SEP-2017) Infectious Diseases, Bacteriology, Novartis
Institutes for Biomedical Research, 5300 Chiron way, Emeryville, CA
94608, USA
REFERENCE 2 (bases 1 to 5845)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 5845)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(15-SEP-2017) Infectious Diseases, Bacteriology, Novartis Institutes
for Biomedical Research, 5300 Chiron way, Emeryville, CA 94608, USA"
COMMENT SGRef: number: 2; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5845
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(38..1117)
/label=lacI
/note="lac repressor"
promoter complement(1118..1195)
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
promoter 1425..1453
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 1461..1477
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 1511..3202
/gene="COASY"
/label=COASY
/note="Bifunctional coenzyme A synthase from Homo sapiens.
Accession#: Q13057"
promoter 3243..3271
/label=Pc promoter
/note="class 1 integron promoter"
CDS 3460..3990
/label=GmR
/note="gentamycin acetyltransferase"
rep_origin 4015..4470
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 4581..5169
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(5355..5495)
/label=bom
/note="basis of mobility region from pBR322"
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