LOCUS       V004295                 5845 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004295
VERSION     V004295
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2017) Infectious Diseases, Bacteriology, Novartis
            Institutes for Biomedical Research, 5300 Chiron way, Emeryville, CA
            94608, USA
REFERENCE   2  (bases 1 to 5845)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5845)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (15-SEP-2017) Infectious Diseases, Bacteriology, Novartis Institutes
            for Biomedical Research, 5300 Chiron way, Emeryville, CA 94608, USA"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(38..1117)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(1118..1195)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        1425..1453
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1461..1477
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1511..3202
                     /gene="COASY"
                     /label="Bifunctional coenzyme A synthase"
                     /note="Bifunctional coenzyme A synthase from Homo sapiens.
                     Accession#: Q13057"
     promoter        3243..3271
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             3460..3990
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     rep_origin      4015..4470
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      4581..5169
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5355..5495)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 caattcgcgc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
       61 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      121 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
      181 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
      241 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
      301 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
      361 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
      421 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
      481 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
      541 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
      601 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
      661 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
      721 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
      781 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
      841 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
      901 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
      961 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     1021 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     1081 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     1141 cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt
     1201 aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc gactgcacgg
     1261 tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg
     1321 taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg
     1381 cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc
     1441 ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaattcg
     1501 agctcggtac atggccgtat tccggtcggg tctcctggtg ctgacgacgc cgctggcctc
     1561 cctagcccct cgcctggcct ccatcctgac ctcggcggcc cggctggtga atcacacact
     1621 ctatgttcac ctgcagccgg gcatgagcct ggagggcccg gctcagcccc agtccagccc
     1681 cgtgcaggcc acgtttgagg ttcttgattt catcacgcac ctctatgctg gcgccgacgt
     1741 ccacaggcac ttggacgtca gaatcctact gaccaatatc cgaaccaaga gcacctttct
     1801 ccctcccctg cccacctcag tccagaatct cgcccacccg ccagaagtcg tgttgacaga
     1861 tttccagacc ctggatggaa gccagtacaa cccggtcaaa cagcagctag tgcgttacgc
     1921 caccagctgt tacagctgtt gtccgcgact ggcctcggtg ctgctatact ccgattatgg
     1981 gataggagaa gtgcccgtgg agcccctgga tgtcccctta ccctccacga tcaggccagc
     2041 ttcccccgtg gccgggtctc caaagcagcc ggtgcgtggc tactaccgtg gcgctgtcgg
     2101 tggcacgttt gaccgcctgc acaacgccca caaggtgttg ctcagtgtcg cgtgcatcct
     2161 ggcccaggag cagcttgtgg tgggagtagc agacaaagat ctgttgaaga gcaagttgct
     2221 ccctgagctg ctccaacctt atacagaacg tgtggaacat ctgagtgaat tcctggtgga
     2281 catcaagccc tccttgactt ttgatgtcat ccccctgctg gacccctatg ggcccgctgg
     2341 ctctgacccc tccctggagt tcctggtggt cagcgaggag acctatcgtg gggggatggc
     2401 catcaaccgc ttccgccttg agaatgacct ggaggaactt gctttgtacc agatccagct
     2461 gctgaaggac ctcagacata cagagaatga agaggacaaa gtcagctcct ccagcttccg
     2521 ccagcgaatg ttggggaacc tgcttcggcc tccatatgaa aggccagagc tccccacatg
     2581 tctctatgta attgggctga ctggcatcag tggctctggg aagagctcaa tagctcagcg
     2641 actgaagggc ctgggggcgt ttgtcattga cagtgaccac ctgggtcatc gggcctatgc
     2701 cccaggtggc cctgcctacc agcctgtggt ggaggccttt ggaacagata ttctccataa
     2761 agatggcatc atcaacagga aggtcctagg cagccgggtg tttgggaata agaagcagct
     2821 gaagatactc acggacatta tgtggccaat tatcgcaaag ctggcccgag aggagatgga
     2881 tcgggctgtg gctgagggaa agcgtgtgtg tgtgattgat gccgctgtgt tgcttgaagc
     2941 cggctggcag aacctggtcc atgaggtgtg gactgctgtc atcccagaga ctgaggctgt
     3001 aagacgcatt gtggagaggg atggcctcag tgaagccgcg gctcaaagcc ggctgcagag
     3061 ccagatgagc gggcagcagc ttgtggaaca gagccacgtg gtgctcagca ccttgtggga
     3121 gccgcatatc acccaacgcc aggtggagaa agcctgggcc ctcttgcaga agcgcattcc
     3181 caagactcat caggccctcg actgaggctg ttttggcgga tgagagaagt accgagctcg
     3241 aattgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg cgctcacgca
     3301 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     3361 cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     3421 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagcaacgat
     3481 gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat gggcatcatt
     3541 cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct tgatcttttc
     3601 ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc cgattacctc
     3661 gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca agaagcggtt
     3721 gttggcgctc tcgcggctta cgttctgccc aggtttgagc agccgcgtag tgagatctat
     3781 atctatgatc tcgcagtctc cggcgagcac cggaggcagg gcattgccac cgcgctcatc
     3841 aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt gcaagcagat
     3901 tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga agaagtgatg
     3961 cactttgata tcgacccaag taccgccacc taacaattcg ctttagattg atttacgcgc
     4021 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4081 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4141 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4201 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     4261 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4321 tgttccaaac ttgaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
     4381 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4441 attttaacaa aatattaacg tttacaattt aaaaggatct aggtgaagat cctttttgat
     4501 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4561 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4621 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4681 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     4741 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4801 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4861 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4921 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4981 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5041 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5101 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5161 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5221 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5281 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5341 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     5401 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     5461 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     5521 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     5581 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaag
     5641 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
     5701 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
     5761 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
     5821 atctgctcat gtttgacagc ttatc
//
