Basic Vector Information
- Vector Name:
- pNICO
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7269 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Belbahri L, Champouret N, Mauch F.
- Promoter:
- T3
pNICO vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNICO vector Sequence
LOCUS 40924_33272 7269 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNICO, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7269)
AUTHORS Belbahri L, Champouret N, Mauch F.
TITLE Versatile cloning vectors for Phytophthora transformation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7269)
AUTHORS Belbahri L, Champouret N, Mauch F.
TITLE Direct Submission
JOURNAL Submitted (31-OCT-2007) Laboratory of Applied Genetics, School of
Engineering of Lullier, 150 Route de Presinge, Jussy 1254,
Switzerland
REFERENCE 3 (bases 1 to 7269)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7269)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(31-OCT-2007) Laboratory of Applied Genetics, School of Engineering
of Lullier, 150 Route de Presinge, Jussy 1254, Switzerland"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7269
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 1011..1575
/label=5' Bremia lactucae Ham34 promoter
/note="5' Bremia lactucae Ham34 promoter"
/regulatory_class="promoter"
primer_bind 1588..1604
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 1670..2383
/label=GFP (S65T)
/note="S65T variant of Aequorea victoria green fluorescent
protein (Heim et al., 1995)"
regulatory 2427..2947
/label=3' Bremia lactucae Ham34 terminator
/note="3' Bremia lactucae Ham34 terminator"
/regulatory_class="terminator"
regulatory 2955..3538
/label=5' Bremia lactucae Hsp70 promoter
/note="5' Bremia lactucae Hsp70 promoter"
/regulatory_class="promoter"
CDS 3554..4345
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
regulatory 4535..4754
/label=5' Bremia lactucae Hsp70 terminator
/note="5' Bremia lactucae Hsp70 terminator"
/regulatory_class="terminator"
promoter complement(5081..5099)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5120..5136)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5144..5160)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5168..5198)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5213..5234)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5522..6110)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6284..7141)
/label=AmpR
/note="beta-lactamase"
promoter complement(7142..7246)
/label=AmpR promoter
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