LOCUS       40924_33272        7269 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNICO, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Belbahri L, Champouret N, Mauch F.
  TITLE     Versatile cloning vectors for Phytophthora transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7269)
  AUTHORS   Belbahri L, Champouret N, Mauch F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2007) Laboratory of Applied Genetics, School of 
            Engineering of Lullier, 150 Route de Presinge, Jussy 1254, 
            Switzerland
REFERENCE   3  (bases 1 to 7269)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7269)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-OCT-2007) Laboratory of Applied Genetics, School of Engineering 
            of Lullier, 150 Route de Presinge, Jussy 1254, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1011..1575
                     /label=5' Bremia lactucae Ham34 promoter
                     /note="5' Bremia lactucae Ham34 promoter"
                     /regulatory_class="promoter"
     primer_bind     1588..1604
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1670..2383
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     regulatory      2427..2947
                     /label=3' Bremia lactucae Ham34 terminator
                     /note="3' Bremia lactucae Ham34 terminator"
                     /regulatory_class="terminator"
     regulatory      2955..3538
                     /label=5' Bremia lactucae Hsp70 promoter
                     /note="5' Bremia lactucae Hsp70 promoter"
                     /regulatory_class="promoter"
     CDS             3554..4345
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      4535..4754
                     /label=5' Bremia lactucae Hsp70 terminator
                     /note="5' Bremia lactucae Hsp70 terminator"
                     /regulatory_class="terminator"
     promoter        complement(5081..5099)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5120..5136)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5144..5160)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5168..5198)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5213..5234)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5522..6110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6284..7141)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7142..7246)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gcccattata cctcgtattc gattaaatga tacaactcat tgccacaccg caagacggcg
      721 tcaccaccat agacagcctt tcaagcaccc ccttgtctat gagctgccac gtcagctgcc
      781 gctcctaata tccacccgcg tccctattcc cgtcatatgg acgtacttga tggtaaaaat
      841 caagggccaa ctgactaccc acggaaaaat cgctatccca tcaaagcagt cgagtttttc
      901 cttttttttt cttatctttt tcaagtcaaa tcaaaatgtc gctaccataa ccattatacc
      961 gccctcaaaa taaagtctat ccctcctatc cactggtcgg ttcctctccc tctgatggac
     1021 aaagggtcgc ctctacgtac ccgcgacaat ctcggctttt ccaatgtcga cagctggcat
     1081 atcgcacgcg aggttaggcg tcttcagccg gcgtaaccac aaacctcgag tcaagtaccc
     1141 tttttcagac cttaatttcc gtgtccattc cctcgcgttt acacgaaggt ttctgagaaa
     1201 taccacatgt cacagagaga gagaagattg gagaaccttc tcaacctgac ccgtgcagag
     1261 cgcttttgcg tcctaccatc cgttaattat agcaccacca ccacatgtct cgccggaact
     1321 ctcttatccg ctctttttga tcgtcttttt tgcgccccat taaaagacat ctcttgcaca
     1381 gggatcgttt ctgattggcc atttcacttc ctcttttaca tccgaaaacc acctgtttgt
     1441 ggggttgtga agccacaacc ggacgctcaa aaagcctcgc ccgactcgcc cacgatcgga
     1501 aggctcattc tccttttcac tctcacgtgc ccaagtccca accgactctt tttcgacctt
     1561 cactctcacc gacaacgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
     1621 attcggcggt ggagggtctg ggggtggcgg aagcggaggg ggtggctcga tgggcaaggg
     1681 cgaggaactg ttcactggcg tggtcccaat cctggtggaa ctggatggtg atgtgaacgg
     1741 gcacaagttc tccgtcagcg gagagggtga aggtgatgcc acctacggaa agctcaccct
     1801 gaagttcatc tgcactaccg gaaagctccc tgttccgtgg ccaaccctcg tcaccacttt
     1861 cacctacggt gttcagtgct tctcccggta cccagatcac atgaagcagc atgacttctt
     1921 caagagcgcc atgcccgaag gctacgtgca agaaaggact atcttcttca aggatgacgg
     1981 gaactacaag acacgtgccg aagtcaagtt cgaaggtgat accctggtga accgcatcga
     2041 gctgaaaggc atcgatttca aggaagatgg aaacatcctt ggacacaagc tggagtacaa
     2101 ctacaactcc cacaacgtat acatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2161 cttcaagatc aggcacaaca tcgaagatgg aagcgtgcaa ctggcggacc actaccagca
     2221 gaacacgccc atcggcgatg gccctgtcct gctgccggac aaccattacc tgtccacgca
     2281 atctgccctc tccaaggacc ccaacgagaa gagggaccac atggtcctgc tggagttcgt
     2341 gacggctgct gggatcacgc atggcatgga tgaactctac aagtgatcta gagcggccgc
     2401 caccgcgggg tgcctacttc ttgtaagccg aaggtcgagc tgtgtgcact gagtgcgaag
     2461 ttgcagattt tgtgtctatg catttaatac aatttccttt tttccatttt cgttgttttt
     2521 ttttgtgaaa gaacgattgt tttcaaaaga aggccgtttg tagaacaatt tttttttata
     2581 ctagcatttg gtattcgagt gtgacgaatc ggtgatggcg ctttacaaat gaaatataac
     2641 gctgaacaag cgcagtgaca gctttatttt ataacagaga ataaagacca aagtagagta
     2701 gactgatggc ggcttgaaac aaaactacgt gacttctaat gcaaagaaaa ccatacccca
     2761 cgctacttag aattgtttct ctttggttga tgaccgctat agtcgtatgg agtgatccct
     2821 ttttgtcaat atttcgattc ttgctaaaac cgtgactatt ttgaggcaac tgtgtgtact
     2881 taacatggta gaaatttcta tgtttaagcc agctgacaat cattctttat ttatcgtaaa
     2941 ccaaccacca tatgcgtcaa tgcaattgac tgtgtcctcg attatttcta ttattaatat
     3001 tatttccatt aaaaaagaaa tccagttcgt ggcaaaataa gacaaaggtt taaataaaaa
     3061 tttatcgtta ttaaactcgt aacttacctt cttcatggct caacccaatc tgtatatcga
     3121 cttatttaat ttggttaaca aatcggtttt cgtcgcaaat aggggatgac aactaaaata
     3181 ccaatttgtg tccttgcgac acgtcaacag gtggctgcca atatctcagg ttttgagcac
     3241 acaacgctgt gattggctat ctgtagatcg cttcgagaac tttcggaccg acatatcaga
     3301 acgcagtcat gtccaatcca gctaacgatt ggtcgaaacc gcacttcatc gcttcatttc
     3361 aaccaattcc catctactaa aacgagcgtc ggaatagtct cgaacgtaac cccacgtagt
     3421 gggtggcagc gcccctcgta tctttcaatc gccactcgag tctcaagttt gctcactttg
     3481 aaattttcca tctggactac acatcaaatc taactcaact tttatcaatt gaaacacaag
     3541 ggccccgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3601 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3661 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3721 cctgaatgaa ctgcaggacg agccagcgcg gctatcgtgg ctggccacga cgggcgttcc
     3781 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     3841 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     3901 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     3961 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4021 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4081 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4141 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4201 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4261 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4321 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     4381 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4441 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4501 ctggagttct tcgcccaccc cccggatccc cggggtcgac tagaatactt agcaattggt
     4561 tagtttgaaa attgtcaaag cagagttttt tcgacaataa aatttatttt ctaaatattt
     4621 gatttagcgt tgacttaatt tgttgattcg gttgtcatct tttgtgaaga tgcccgagag
     4681 tctagtaaat ctcaagcgaa acatagtgag aaggtcacta tggctggttt gcatgagtct
     4741 gggcctattg tgcttacgcc gccttagtaa caaacatgcc aaaagaagga aagctttcac
     4801 cagctttcaa gtttaatcaa tctttgagag tcacaattgc aagaataaat gaatttttac
     4861 aacacagttc gatgcactgt gtttcgtctt gatgaaaatt ggacggcaaa tcgagtatct
     4921 taaacaaatc tcggcatagg ctgtccattt tgtggtcgcc tctttgttgg tagtttgcag
     4981 tagtttcatt agtagaagat ctgcatcctc aatctgctaa acgtcttgag cagctatgag
     5041 acgatccggg gaattggggc ccgagctcca gcttttgttc cctttagtga gggttaattg
     5101 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5161 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5221 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5281 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5341 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5401 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5461 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5521 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5581 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5641 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5701 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5761 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5821 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5881 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5941 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     6001 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6061 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6121 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6181 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6241 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6301 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6361 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6421 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6481 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6541 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6601 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6661 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6721 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6781 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6841 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6901 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6961 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7021 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7081 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7141 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7201 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7261 aagtgccac
//
