Basic Vector Information
- Vector Name:
- pNG1A
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4065 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Gomez-Santos N, Treuner-Lange A, Moraleda-Munoz A, Garcia-Bravo E, Garcia-Hernandez R, Martinez-Cayuela M, Perez J, Sogaard-Andersen L, Munoz-Dorado J.
pNG1A vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNG1A vector Sequence
LOCUS 40924_33152 4065 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNG1A, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4065)
AUTHORS Gomez-Santos N, Treuner-Lange A, Moraleda-Munoz A, Garcia-Bravo E,
Garcia-Hernandez R, Martinez-Cayuela M, Perez J, Sogaard-Andersen L,
Munoz-Dorado J.
TITLE Comprehensive Set of Integrative Plasmid Vectors for
Copper-Inducible Gene Expression in Myxococcus xanthus
JOURNAL Appl. Environ. Microbiol. 78 (8), 2515-2521 (2012)
PUBMED 22287008
REFERENCE 2 (bases 1 to 4065)
AUTHORS Gomez-Santos N.
TITLE Direct Submission
JOURNAL Submitted (26-OCT-2011) Microbiology, University of Granada (Spain),
Avda. Fuentenueva s/n, Granada, Granada 18071, Spain
REFERENCE 3 (bases 1 to 4065)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4065)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
Environ. Microbiol."; date: "2012"; volume: "78"; issue: "8"; pages:
"2515-2521"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-OCT-2011) Microbiology, University of Granada (Spain), Avda.
Fuentenueva s/n, Granada, Granada 18071, Spain"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4065
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 7..973
/label=cuoA promoter
/note="cuoA promoter"
/regulatory_class="promoter"
misc_feature 974..1008
/label=multicloning site
/note="multicloning site"
primer_bind complement(1021..1037)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(1045..1061)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1069..1099)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(1114..1135)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1423..2011)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 2492..3283
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
primer_bind 4037..4053
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
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