LOCUS       40924_33152        4065 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNG1A, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Gomez-Santos N, Treuner-Lange A, Moraleda-Munoz A, Garcia-Bravo E, 
            Garcia-Hernandez R, Martinez-Cayuela M, Perez J, Sogaard-Andersen L,
            Munoz-Dorado J.
  TITLE     Comprehensive Set of Integrative Plasmid Vectors for 
            Copper-Inducible Gene Expression in Myxococcus xanthus
  JOURNAL   Appl. Environ. Microbiol. 78 (8), 2515-2521 (2012)
  PUBMED    22287008
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Gomez-Santos N.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2011) Microbiology, University of Granada (Spain),
            Avda. Fuentenueva s/n, Granada, Granada 18071, Spain
REFERENCE   3  (bases 1 to 4065)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4065)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "8"; pages:
            "2515-2521"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-OCT-2011) Microbiology, University of Granada (Spain), Avda. 
            Fuentenueva s/n, Granada, Granada 18071, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..973
                     /label=cuoA promoter
                     /note="cuoA promoter"
                     /regulatory_class="promoter"
     misc_feature    974..1008
                     /label=multicloning site
                     /note="multicloning site"
     primer_bind     complement(1021..1037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1045..1061)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1069..1099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1114..1135)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1423..2011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2492..3283
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     4037..4053
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtaccgctg cttcaacggc attcatgggg cttcctcctg cccgcgagct agcgggtcac
       61 cccactgacg cgcctccccc gaaaggctta cacctgcgaa gagggccagc cgcggtggcc
      121 agcgcctcgt gcgccacccg cagcgtgtcg gccatgaacg gcacttcacg gctccgtgga
      181 acgacgctgc tccacgttcc acctgtgacc gtgacggtta cagcgaaacc catccgggta
      241 ctccggacag gtgccgcgcc acggcaaccc cgcatgaaca ggctcgctca gccctcggca
      301 cgcgttgtgc tccagcggac cgacttcacg ggatgccgga cgtcctcgag agcatcctca
      361 ctctgggagc aaccaagcga tgaagaagag cctgatggga ctccttgctg cactccccct
      421 gattgccgcg gcccccgcgg tcgcgtgtga cgagcacgcc aagcacggcg agcacgccag
      481 ccacggcgag cacgccaagc acggcgatca ttccgcgctc ggaggcacgg ccaagaaggc
      541 cgctgcgccc gcacagaagc ccgcctcggc cccggccacc aggaatggcg tccagaccgt
      601 ggacctcacg gtgaccccca agggcttcga gccctcggac gtgaaggtga aggcggggca
      661 gcccgtccgc ctcgtggtca cgcgcaagac ggccaagacg tgcgccacgg aaatcgtcat
      721 ggccgacctg ggcatcaaca agccgctccc catggacacg ccggtgaccg tggagttcac
      781 ccccagcaag tcgggcacgc tgcgctacgc ctgcgcgatg gaccacatca gcgggctcgt
      841 caccatccag tagaccgccg ttccgggcag gctgcgcacc gaggtcacgc gcctcggggc
      901 cgggccacgc caccacggcg gcctggtccc ctcctcgccc gcagcttcca tccattcgtg
      961 aagaggcttc atggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg
     1021 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1081 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1141 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1201 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1261 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1321 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1381 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1441 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1561 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1621 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1681 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1741 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1861 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1921 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1981 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2041 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2101 gatcttcacc tagatccttt ttcgacctgc agcccgaccc ttcacgctgc cgcaagcact
     2161 cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt
     2221 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     2281 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     2341 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     2401 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg gatcaagatc
     2461 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     2521 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     2581 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     2641 gaccgacctg tccggtgccc tgaatgaact ccaggacgag gcagcgcggc tatcgtggct
     2701 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     2761 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     2821 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     2881 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     2941 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     3001 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     3061 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     3121 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     3181 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     3241 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     3301 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     3361 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     3421 cagcgcgggg aaaacatgct ggagttcttc gcccaccggt cgggctgcag gtcgaattat
     3481 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3541 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3601 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     3661 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     3721 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     3781 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     3841 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     3901 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     3961 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     4021 tcccagtcac gacgttgtaa aacgacggcc agtgaattcg agctc
//
