Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V004337 | pNFkB-Luc | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
NFkB reporter vector is utilized for detecting NFkB (Nuclear factor of kappa light polypeptide gene) transactivation in a cell-based assay.
- Vector Name:
- pNFkB-Luc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5695 bp
- Type:
- Reporter vector
- Replication origin:
- ori
- Source/Author:
- Zheng C-F.
- Selection Marker:
- Fluc
- Promoter:
- minP
pNFkB-Luc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNFkB-Luc vector Sequence
LOCUS 40924_33133 5695 bp DNA circular SYN 16-JAN-2024 DEFINITION Reporter vector pNFkB-Luc, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5695) AUTHORS Zheng C-F. TITLE Direct Submission JOURNAL Submitted (11-MAR-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA REFERENCE 2 (bases 1 to 5695) AUTHORS Zheng C-F. TITLE Direct Submission JOURNAL Submitted (20-JAN-1999) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA REFERENCE 3 (bases 1 to 5695) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (11-MAR-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (20-JAN-1999) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA" FEATURES Location/Qualifiers source 1..5695 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 32..136 /label=AmpR promoter CDS 137..994 /label=AmpR /note="beta-lactamase" rep_origin 1168..1755 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(1934..2074) /label=bom /note="basis of mobility region from pBR322" polyA_signal 2313..2445 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal 2539..2671 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" misc_feature 2680..2746 /label=NFkB response elements promoter 2757..2788 /label=minP /note="minimal TATA-box promoter with low basal activity" CDS 2815..4464 /label=luciferase /note="firefly luciferase" intron 4598..4663 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4854..5210 /codon_start=1 /label=SV40 T-ANTIGEN /translation="MLCLVIELLLALLFTTTKEKAALLYKKIMEKYSVTFISRHNSYNH NILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFT CFKKPPTPPPEPET" polyA_signal 5233..5367 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal"