LOCUS       40924_33133        5695 bp DNA     circular SYN 16-JAN-2024
DEFINITION  Reporter vector pNFkB-Luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-1999) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 5695)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-MAR-1998) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-1999) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
FEATURES             Location/Qualifiers
     source          1..5695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..136
                     /label=AmpR promoter
     CDS             137..994
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1168..1755
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1934..2074)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     polyA_signal    2313..2445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2539..2671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2680..2746
                     /label=NFkB response elements
     promoter        2757..2788
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2815..4464
                     /label=luciferase
                     /note="firefly luciferase"
     intron          4598..4663
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4854..5210
                     /codon_start=1
                     /label=SV40 T-ANTIGEN
                     /translation="MLCLVIELLLALLFTTTKEKAALLYKKIMEKYSVTFISRHNSYNH
                     NILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     polyA_signal    5233..5367
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
     1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
     2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttcact gcattctagt
     2401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcagcca tatcacattt
     2461 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2521 atgaatgcaa ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2581 agcatcacaa atttcacaaa taaagcaatt tttcactgca ttctattgtg gtttgtccaa
     2641 actcatcaat gtatcttatc atgtctggat ccaagctagg ggactttccg cttggggact
     2701 ttccgctggg gactttccgc tggggacttt ccgctgggga ctttccgcgg tgactctaga
     2761 gggtatataa tggaagctcg aattccagct tggcattccg gtactgttgg taaaatggaa
     2821 gacgccaaaa acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct
     2881 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
     2941 acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg
     3001 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
     3061 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
     3121 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag
     3181 cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa
     3241 ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag
     3301 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta
     3361 ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact
     3421 gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc
     3481 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
     3541 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
     3601 gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt
     3661 caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac
     3721 aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa
     3781 gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc
     3841 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
     3901 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
     3961 ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta
     4021 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
     4081 atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa gtctttaatt
     4141 aaatacaaag gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc
     4201 aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga acttcccgcc
     4261 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
     4321 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
     4381 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
     4441 aagaagggcg gaaagtccaa attgtaaaat gtaactgtat tcagcgatga cgaaattctt
     4501 agctattgta atactctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     4561 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     4621 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     4681 ttatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     4741 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc tcaaaagaag
     4801 agaaaggtag agacccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     4861 gtttagtaat agaactcttg cttgctttgc tatttacaac cacaaaggaa aaagctgcac
     4921 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     4981 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     5041 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     5101 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     5161 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     5221 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5281 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5341 atcaatgtat cttatcatgt ctggatcccc aggaagctcc tctgtgtcct cataaaccct
     5401 aacctcctct acttgagagg acattccaat cataggctgc ccatccaccc tctgtgtcct
     5461 cctgttaatt aggtcactta acaaaaagga aattgggtag gggtttttca cagaccgctt
     5521 tctaaggggt aattttaaaa tatctgggaa gtcccttcca ctgctgtgtt ccagaagtgt
     5581 tggtaaacag cccacaaatg tcaacagcag aaacatacaa gctgtcactt tgcacaaagg
     5641 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tctta
//
