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pNDC_SUB4 vector (V004338)

Basic Vector Information

Vector Name:
pNDC_SUB4
Antibiotic Resistance:
Kanamycin
Length:
9246 bp
Type:
Expression vector
Replication origin:
oriV
Source/Author:
De Coi N.

pNDC_SUB4 vector Vector Map

pNDC_SUB49246 bp400800120016002000240028003200360040004400480052005600600064006800720076008000840088009200KanRtrfALB T-DNA repeatLB T-DNA repeat; left border repeat from nopaline C58 T-DNAM13 fwdHygRsub4RB T-DNA repeatoriV

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNDC_SUB4 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33118        9246 bp DNA     circular SYN 14-DEC-2018
DEFINITION  Expression vector pNDC_SUB4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   De Coi N.
  TITLE     Whole gene expression profiles of dermatophytes during infection and
            their virulance traits
  JOURNAL   Thesis (2015) Centre Hospitalier Universitaire Vaudois
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   De Coi N.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2015) Department of Dermatology, Centre 
            Hospitalier de Lausanne (CHUV), Bugnon 48, 1011 Lausanne, 
            SWITZERLAND
REFERENCE   3  (bases 1 to 9246)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9246)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Thesis 
            (2015) Centre Hospitalier Universitaire Vaudois"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAY-2015) Department of Dermatology, Centre Hospitalier de 
            Lausanne (CHUV), Bugnon 48, 1011 Lausanne, SWITZERLAND"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             273..1064
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1366..2511
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     misc_feature    2640..2664
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2698..2722
                     /note="LB T-DNA repeat; left border repeat from nopaline
                     C58 T-DNA"
     primer_bind     2958..2974
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3846..4868)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     CDS             complement(6263..7486)
                     /codon_start=1
                     /product="subtilisin-like protease SUB4"
                     /label=subtilisin-like protease SUB4
                     /note="sub4"
                     /protein_id="CRN13537.1"
                     /translation="MVCLKTLSVFLAAFAAADARAVFKTQGHKNSEMIPDNYIVVMKDG
                     VSQDDFKAHISSVASIHSTNKAKRGTNTQGMKREFDIMNWRGYHGHFDRDTLEEILNDS
                     KVDYVEQDQVVRISGLVTQRSAPSWGLGRVSHRQAGSRDYVFDDSAGRGVTIYGVDTGI
                     DINHQDFRGRARWGTNTADRDNADRHGHGTHTASTFAGTAYGIAKNANIVAVKVLGSDG
                     SGSTSGIIAGINYCVQDAQQRGILGKAAMNLSLGGGFSQANNDAVTRAQNAGIFVAVAA
                     GNDNRDARNYSPASAPAVCTVASSTINDSKSSFSNWGPVELKANITSVDIYAPGSDIIA
                     ARPGGGSTTMSGTSMASPHVAGMGAYMIGMGADPRQVCDRLKQLATAAIRNPGSSTTNR
                     LLYNGSGQ"
     misc_feature    8443..8467
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8606..9237
                     /label=oriV
                     /note="incP origin of replication"

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