LOCUS       40924_33118        9246 bp DNA     circular SYN 14-DEC-2018
DEFINITION  Expression vector pNDC_SUB4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   De Coi N.
  TITLE     Whole gene expression profiles of dermatophytes during infection and
            their virulance traits
  JOURNAL   Thesis (2015) Centre Hospitalier Universitaire Vaudois
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   De Coi N.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2015) Department of Dermatology, Centre 
            Hospitalier de Lausanne (CHUV), Bugnon 48, 1011 Lausanne, 
            SWITZERLAND
REFERENCE   3  (bases 1 to 9246)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9246)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Thesis 
            (2015) Centre Hospitalier Universitaire Vaudois"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAY-2015) Department of Dermatology, Centre Hospitalier de 
            Lausanne (CHUV), Bugnon 48, 1011 Lausanne, SWITZERLAND"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             273..1064
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1366..2511
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     misc_feature    2640..2664
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2698..2722
                     /note="LB T-DNA repeat; left border repeat from nopaline
                     C58 T-DNA"
     primer_bind     2958..2974
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3846..4868)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     CDS             complement(6263..7486)
                     /codon_start=1
                     /product="subtilisin-like protease SUB4"
                     /label=subtilisin-like protease SUB4
                     /note="sub4"
                     /protein_id="CRN13537.1"
                     /translation="MVCLKTLSVFLAAFAAADARAVFKTQGHKNSEMIPDNYIVVMKDG
                     VSQDDFKAHISSVASIHSTNKAKRGTNTQGMKREFDIMNWRGYHGHFDRDTLEEILNDS
                     KVDYVEQDQVVRISGLVTQRSAPSWGLGRVSHRQAGSRDYVFDDSAGRGVTIYGVDTGI
                     DINHQDFRGRARWGTNTADRDNADRHGHGTHTASTFAGTAYGIAKNANIVAVKVLGSDG
                     SGSTSGIIAGINYCVQDAQQRGILGKAAMNLSLGGGFSQANNDAVTRAQNAGIFVAVAA
                     GNDNRDARNYSPASAPAVCTVASSTINDSKSSFSNWGPVELKANITSVDIYAPGSDIIA
                     ARPGGGSTTMSGTSMASPHVAGMGAYMIGMGADPRQVCDRLKQLATAAIRNPGSSTTNR
                     LLYNGSGQ"
     misc_feature    8443..8467
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8606..9237
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ctcccatccc cccaggcgta cgccactgga gcacctcaaa aacaccatca tacactaaat
       61 cagtaagttg gcagcatcac ccataattgt ggtttcaaaa tcggctccgt cgatactatg
      121 ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag
      181 aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt
      241 aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat
      301 tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta
      361 aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt
      421 ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa
      481 agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca
      541 tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa
      601 agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt
      661 gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg
      721 atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg
      781 agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg
      841 acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg
      901 ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg
      961 aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg
     1021 attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac ctagatgtgg
     1081 cgcaacgatg ccggcgacaa gcaggagcgc accgacttct tccgcatcaa gtgttttggc
     1141 tctcaggccg aggcccacgg caagtatttg ggcaaggggt cgctggtatt cgtgcagggc
     1201 aagattcgga ataccaagta cgagaaggac ggccagacgg tctacgggac cgacttcatt
     1261 gccgataagg tggattatct ggacaccaag gcaccaggcg ggtcaaatca ggaataaggg
     1321 cacattgccc cggcgtgagt cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt
     1381 gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga ggatgccgaa
     1441 accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg
     1501 atggtccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc tccccctgcc
     1561 ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc tcgaacagga ggcggcaggt
     1621 ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc
     1681 gccggcgagg acctggcaaa acaggtcagc gaggccaagc aggccgcgtt gctgaaacac
     1741 acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc gtggccggac
     1801 acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac gcgcaacaag
     1861 aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag
     1921 atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg
     1981 ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt ctacgagctt
     2041 tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga ggaatgcctg
     2101 tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca cctggaatcg
     2161 gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag
     2221 gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac gaaattcata
     2281 tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta tttcagctcg
     2341 caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg atcggattcc
     2401 acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc
     2461 ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg ctagggcctt
     2521 gtggggtcag ttccggctgg atctgctctc ccgctgacgc cgtcccggac tgatgggctg
     2581 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     2641 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttatggc tggctggtgg
     2701 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
     2761 gtttttaatg tactggggtg gtttttcttt tcaccagtga gacgggcaac agcggcgcca
     2821 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     2881 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     2941 ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta ccaaggcccg
     3001 ggctggccac ggccgcctag gcgcgcaagg atcctctaga aagaaggatt acctctaaac
     3061 aagtgtacct gtgcattctg ggtaaacgac tcataggaga gttgtaaaaa agtttcggcc
     3121 ggcgtattgg gtgttacgga gcattcacta ggcaaccatg gttactattg tatacccatc
     3181 ttagtaggaa tgattttcga ggtttatacc tacgatgaat gtgtgtcctg taggcttgag
     3241 agttcaagga agaaacagtg caattatctt tgcgaaccca ggggctggtg acggaatttt
     3301 catagtcaag ctatcagagt aaagaagagg agcatgtcaa agtacaatta gagacaaata
     3361 tatagtcgcg tggagccaag agcggattcc tcagtctcgt agagtacaat tagagacaaa
     3421 tatatagtcg cgtggagcca agagcggatt cctcagtctc gtaggtctct tgacgaccgt
     3481 tgatctgctt gatctcgtct cccgaaaatg aaaatagact ctgctaagct attcttctgc
     3541 ttcgccggag cctgaagggc gtactagggt tgcgaggtcc aatgcattaa tgcattgcag
     3601 atgagctgta tctggaagag gtaaacccga aacgcgtttt attcttgttg acatggagct
     3661 attaaatcac tagaaggcac tctttgctgc ttggacaaat gaacgtatct tatcgagatc
     3721 ctgaacacca tttgtctcaa ctccggagct gacatcgaca ccaacgatct tatatccaga
     3781 ttcgtcaagc tgtttgatga tttcagtaac gttaagtgga tcgatcccgg tcggcatcta
     3841 ctctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
     3901 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
     3961 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
     4021 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
     4081 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
     4141 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
     4201 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
     4261 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
     4321 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
     4381 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
     4441 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
     4501 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc
     4561 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
     4621 aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
     4681 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     4741 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     4801 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     4861 tttttcatat cgatggcgat tgtaggctga gcacctgttg cactatatca aagggttaga
     4921 tatcgttaag ctccgagggc tggtaagggc agaagggttg cgactgggtg atttgaggtg
     4981 gtatggggta ttggcaggca ggagaagacg gttgacaaga cagacgacac gaggcacgct
     5041 gcagggcacg agacgtcact ctggtggcta tctctgtggt ggtgatatgg tcgagatagc
     5101 ggacagagag agggaggcgc gcggtggtgg ggacgtacca aggactatga cactggcgcg
     5161 gggcagattg tgcaggtcac aggagggcaa accagagggt ctgggaggag gagagggagt
     5221 caacagaggc ggcagatgtg ttgagaagct cacagaaggc cgacgagggc aatgggggaa
     5281 gaaaagctgg ctggcggtgg gatggttcat gccccaccaa ccacggctgt gacgttggag
     5341 ccgggggacc agggcacaca gtggctgctg ggctgctgca ttcccattcc catcgtggtc
     5401 gagctacaag acgcgcgccg ccagcttgac cgctctgcag aggtttgctg ggccggtcaa
     5461 catgatgtca ggccattttc atatggcaat gcgcaggtgg gccttgacat gtgcagccgg
     5521 tggagcggcg tcgacagatc cacgcgtcgc aatcaggcct gcggcagata tggccattga
     5581 ccgcttatgc cgtgcatttt gatcacgggc agccttggtg gaatatcgca cttcttccct
     5641 tgtgcctgtt tttgtggtag tcgctcggag tgtgtgggat gttgatagcc atcgaggctg
     5701 ctggcgagtg aggtttcgtc gggcgcattg tgtgagatat cgacggaggg gcgtatggag
     5761 ggacggagga agggagttca tggctgcagt gatcaatggc ttggggaaag ctgccggtta
     5821 ataaatagta ctccgtaggc taggtggact tggtgagcgt cgacccagcc tgcgatggtt
     5881 gagatatcta agtttagtta cgactggcgg ccatgtttgc catgtttgca cacaacaaaa
     5941 accttaaacc attactttga gtttacgaac aaatactccg tagaggacaa gacaaaagat
     6001 gaagatggag atgggatttt gatttgaaag ttcatgtcaa ttaaaagccg gttttgtatc
     6061 taatgtgatg tgaagagggg gagagtggta ttttcgtgag tttctcaaat gcccgcaaga
     6121 aaaaaacacc tggggataaa taagagtaaa aaaccagcat ctctttttta ctttcaaaca
     6181 tcaaaaaccc tttttttgtt catcaccatc atagtttgtc gttacatcaa gaagtaggtg
     6241 agatgatact agtagatctt tattactggc cgcttccgtt gtagaggagg cggttggtgg
     6301 tgctggaacc agggttgcgg atggcagcgg tagcgagctg cttgaggcgg tcgcagacct
     6361 ggcgggggtc ggcgcccatt ccgatcatgt aggcacccat accagcgacg tgaggagagg
     6421 ccatggaggt accggacatg gtagtgctgc caccaccggg gcgagcagca atgatgtcgg
     6481 agccaggagc atagatgtca actgaagtaa tgttagcttt cagctcaact gggccccagt
     6541 tggagaagga ggatttgctg tcgttgatgg tagaagaggc aacggtgcag acagctggag
     6601 cagaggcagg ggagtagtta cgggcgtctc tgttgtcatt accagcagcg acggcaacga
     6661 agataccggc gttctgggca cgagtaacag catcgttgtt ggcctgactg aagccaccac
     6721 cgagagacaa gttcattgca gcctttccaa ggataccacg ctgttgagca tcctggacac
     6781 agtagttgat accagcaatg attccactgg tgctaccaga gccatcggag ccaaggacct
     6841 taacggcaac aatgttggcg ttcttagcaa taccataagc agtaccagcg aaggtagatg
     6901 cagtgtgggt gccgtggccg tgacgatcag cgttatcacg gtcagcggtg ttagtacccc
     6961 aacgagcacg tccacggaag tcctggtggt taatatcaat accggtatca acaccgtaga
     7021 tggtgacacc acggccggcg gagtcgtcga agacgtagtc acggctacca gcctgtcggt
     7081 gagagacacg tccgagaccc cagctgggag cactgcgctg agtgacaagg ccagagattc
     7141 tcacgacctg gtcctgctca acgtaatcga ccttggagtc attgaggatc tcctcgaggg
     7201 tgtcacggtc gaagtggccg tggtaacctc tccagttcat gatgtcgaat tcgcgcttca
     7261 ttccctgagt gttggtgcct cgcttggcct tgttggtgct gtggatgctg gcaacggagg
     7321 agatgtgggc cttgaagtca tcttgggaga caccatcctt catgacaaca atgtagttgt
     7381 ctggaatcat ttcgctgttc ttgtggccct gagtcttgaa gactgcgcgg gcatcagcgg
     7441 cagcgaatgc cgcaaggaac acggacagag tcttaaggca gaccatcatg atgacggtgt
     7501 tttttgtgtg tggtttgaag tcttgtagct ctttggaaga tactagaaga attcagcaga
     7561 acgctcagat actacggccg aaattgcgta gaggtgctgg agaagcgagt tagctatcgt
     7621 ggcaggacga cgatgatgac ggagagggag aggaagggga gcgtgtgagg gcgagggaag
     7681 acgtactgaa gcagacgaag gagagaaaga aaaaaaagag gaagagaaaa gaattttctg
     7741 tgtgaaggcg acgagcgagg ggtccttttg tatggcgggg cgaggggcgc ggacagccga
     7801 aagaggacta gcgagacggc gctaagccta accgggcagg cagcggaggg gcatgctggg
     7861 cccccaccat gaaaactctg acggcgctta actacctaat gtagaagata aggtgaagat
     7921 ggaggtgaga gagacggtga ggtgacagcg aaggtggata agcagcgcaa ggccgaagga
     7981 gtgtctgata gaatagctga ctaggtgact actacgttct acagatgttg gcaactagct
     8041 agtcgagagc agaagcagtg tatatggcga ccgctgtgag tgtgtgcatg tgtgtgtgtg
     8101 cgtttgtgca caactctgcg tccatccctg caagcccttt ctgatcagcg ccgccgtgcc
     8161 ttggaggggt cgcggtcgag tgcatccagt ttacgtgtcc gagtgggctg tcccggtggg
     8221 ttagcttgaa gagtcttacc gagtgggctg tcccggtggg ttagcttgaa gagtcttagt
     8281 ggcgtggtag actagtgcgc gatcgcggcc ggccggcgcg ccgtttaaac ggatttaaat
     8341 taattaatgt cgacctgcag gcatgcaagc ttcgtgactc ccttaattct ccgctcatga
     8401 tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg
     8461 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     8521 aggtttatcc gttcgtccat ttgtttgttc atgccaacca cagggttcca gatccgacga
     8581 gcaaggcaag accgagcgcc tttccgacgc tcaccgggct ggttgccctc gccgctgggc
     8641 tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac
     8701 accgcggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag
     8761 gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc
     8821 aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct
     8881 gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt
     8941 attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg
     9001 ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggct
     9061 gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt
     9121 aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg
     9181 cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac
     9241 gcgggc
//
