Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V004342 | pNCO113-hisactophilin | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pNCO113-hisactophilin
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3776 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Kay CW, Schleicher E, Kuppig A, Hofner H, Rudiger W, Schleicher M, Fischer M, Bacher A, Weber S, Richter G.
- Promoter:
- T5
pNCO113-hisactophilin vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNCO113-hisactophilin vector Sequence
LOCUS 40924_33098 3776 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pNCO113-hisactophilin, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3776)
AUTHORS Kay CW, Schleicher E, Kuppig A, Hofner H, Rudiger W, Schleicher M,
Fischer M, Bacher A, Weber S, Richter G.
TITLE Blue light perception in plants. Detection and characterization of a
light-induced neutral flavin radical in a C450A mutant of
phototropin
JOURNAL J. Biol. Chem. 278 (13), 10973-10982 (2003)
PUBMED 12525505
REFERENCE 2 (bases 1 to 3776)
AUTHORS Fischer MJ, Bacher A.
TITLE Direct Submission
JOURNAL Submitted (09-SEP-2002) Organic Chemistry and Biochemistry,
Technical University Munich, Lichtenbergstr. 4, Garching 85747,
Germany
REFERENCE 3 (bases 1 to 3776)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3776)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol.
Chem."; date: "2003"; volume: "278"; issue: "13"; pages:
"10973-10982"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(09-SEP-2002) Organic Chemistry and Biochemistry, Technical
University Munich, Lichtenbergstr. 4, Garching 85747, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3776
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 10..54
/label=T5 promoter
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 62..78
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 97..108
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 115..468
/gene="hatA"
/label=hatA
/note="Hisactophilin-1 from Dictyostelium discoideum.
Accession#: P13231"
terminator 518..612
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
terminator 1384..1470
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 1626..1766
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(1952..2540)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2714..3571)
/label=AmpR
/note="beta-lactamase"
promoter complement(3572..3676)
/label=AmpR promoter