LOCUS       V004342                 3776 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004342
VERSION     V004342
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Kay CW, Schleicher E, Kuppig A, Hofner H, Rudiger W, Schleicher M,
            Fischer M, Bacher A, Weber S, Richter G.
  TITLE     Blue light perception in plants. Detection and characterization of a
            light-induced neutral flavin radical in a C450A mutant of
            phototropin
  JOURNAL   J. Biol. Chem. 278 (13), 10973-10982 (2003)
   PUBMED   12525505
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Fischer MJ, Bacher A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2002) Organic Chemistry and Biochemistry,
            Technical University Munich, Lichtenbergstr. 4, Garching 85747,
            Germany
REFERENCE   3  (bases 1 to 3776)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3776)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol.
            Chem."; date: "2003"; volume: "278"; issue: "13"; pages:
            "10973-10982"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-SEP-2002) Organic Chemistry and Biochemistry, Technical
            University Munich, Lichtenbergstr. 4, Garching 85747, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             115..468
                     /gene="hatA"
                     /label="Hisactophilin-1"
                     /note="Hisactophilin-1 from Dictyostelium discoideum.
                     Accession#: P13231"
     terminator      518..612
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      1384..1470
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1626..1766
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1952..2540)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2714..3571)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3572..3676)
                     /label="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatgggt
      121 aacagagcat tcaaatcaca tcacggtcac tttttaagcg ctgaaggcga agctgtaaag
      181 actcaccacg gtcatcatga tcatcacacc catttccacg ttgaaaacca tggtggtaaa
      241 gttgcattaa agacccattg cggtaaatac ctttcaattg gtgatcataa acaagtttac
      301 ctctcacacc acttacacgg tgaccactca ctcttccact tagaacatca tggcggtaaa
      361 gtctcaatca aaggtcatca ccaccactac atttccgctg atcatcatgg tcatgtttca
      421 accaaagaac accacgatca cgacaccacc tttgaagaaa ttattattgg atccgcggcc
      481 gcatcgtctg gttcctaagc ttaattagct gagcttggac tcctgttgat agatccagta
      541 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
      601 attggtgaga atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga
      661 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
      721 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
      781 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
      841 tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct
      901 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
      961 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     1021 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     1081 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     1141 tatggacaac ttcttcgccc ccgttttcac catgcatggg caaatattat acgcaaggcg
     1201 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg
     1261 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
     1321 ttttttaagg cagttattgg tgcccttaaa cgcctggggt aatgactctc tagcttgagg
     1381 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1441 tcggtgaacg ctctcctgag taggacaaat ccgccgctct agagctgcct cgcgcgtttc
     1501 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     1561 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     1621 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     1681 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     1741 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     1801 gctcggtctg tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     2161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     2401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2761 gttcatccat agctgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     3721 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcac
//
