Basic Vector Information
- Vector Name:
- pNCO113-hisactophilin-C49S
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3792 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Eisenreich W, Joshi M, Illarionov B, Richter G, Romisch-Margl W, Muller F, Bacher A, Fischer M.
- Promoter:
- T5
pNCO113-hisactophilin-C49S vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNCO113-hisactophilin-C49S vector Sequence
LOCUS 40924_33093 3792 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pNCO113-hisactophilin-C49S, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3792)
AUTHORS Eisenreich W, Joshi M, Illarionov B, Richter G, Romisch-Margl W,
Muller F, Bacher A, Fischer M.
TITLE (13)C Isotopologue editing of FMN bound to phototropin domains
JOURNAL FEBS J. 274 (22), 5876-5890 (2007)
PUBMED 17944933
REFERENCE 2 (bases 1 to 3792)
AUTHORS Fischer M.
TITLE Direct Submission
JOURNAL Submitted (05-MAR-2007) Chemistry, University of Hamburg,
Grindelallee 117, Hamburg 20146, Germany
REFERENCE 3 (bases 1 to 3792)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3792)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J.";
date: "2007"; volume: "274"; issue: "22"; pages: "5876-5890"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(05-MAR-2007) Chemistry, University of Hamburg, Grindelallee 117,
Hamburg 20146, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3792
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 10..54
/label=T5 promoter
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 62..78
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 97..108
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 115..468
/gene="hatA"
/label=hatA
/note="Hisactophilin-1 from Dictyostelium discoideum.
Accession#: P13231"
CDS 487..504
/label=thrombin site
/note="thrombin recognition and cleavage site"
terminator 534..628
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
terminator 1400..1486
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 1642..1782
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(1968..2556)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2730..3587)
/label=AmpR
/note="beta-lactamase"
promoter complement(3588..3692)
/label=AmpR promoter
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