LOCUS       V004343                 3792 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004343
VERSION     V004343
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Eisenreich W, Joshi M, Illarionov B, Richter G, Romisch-Margl W,
            Muller F, Bacher A, Fischer M.
  TITLE     (13)C Isotopologue editing of FMN bound to phototropin domains
  JOURNAL   FEBS J. 274 (22), 5876-5890 (2007)
   PUBMED   17944933
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Fischer M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2007) Chemistry, University of Hamburg,
            Grindelallee 117, Hamburg 20146, Germany
REFERENCE   3  (bases 1 to 3792)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3792)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J.";
            date: "2007"; volume: "274"; issue: "22"; pages: "5876-5890"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-MAR-2007) Chemistry, University of Hamburg, Grindelallee 117,
            Hamburg 20146, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             115..468
                     /gene="hatA"
                     /label="Hisactophilin-1"
                     /note="Hisactophilin-1 from Dictyostelium discoideum.
                     Accession#: P13231"
     CDS             487..504
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     terminator      534..628
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      1400..1486
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1642..1782
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1968..2556)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2730..3587)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3588..3692)
                     /label="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatgggt
      121 aacagagcat tcaaatcaca tcacggtcac tttttaagcg ctgaaggcga agctgtaaag
      181 actcaccacg gtcatcatga tcatcacacc catttccacg ttgaaaacca tggtggtaaa
      241 gttgcattaa agacccattc cggtaaatac ctttcaattg gtgatcataa acaagtttac
      301 ctctcacacc acttacacgg tgaccactca ctcttccact tagaacatca tggcggtaaa
      361 gtctcaatca aaggtcatca ccaccactac atttccgctg atcatcatgg tcatgtttca
      421 accaaagaac accacgatca cgacaccacc tttgaagaaa ttattattgg ttccgcggca
      481 tctggtctgg tgccacgcgg atccggggta gtaagcttaa ttagctgagc ttggactcct
      541 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
      601 ccgccgggcg ttttttattg gtgagaatcc aagctagctt ggcgagattt tcaggagcta
      661 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      721 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      781 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      841 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa
      901 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      961 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     1021 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     1081 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     1141 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg catgggcaaa
     1201 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc
     1261 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     1321 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg
     1381 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1441 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctctagag
     1501 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     1561 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     1621 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     1681 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     1741 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     1801 ctcactgact cgctgcgctc ggtctgtcgg ctgcggcgag cggtatcagc tcactcaaag
     1861 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1921 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1981 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2041 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2101 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2161 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2221 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2281 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2341 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2401 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2461 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2521 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2581 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2641 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2701 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2761 gcgatctgtc tatttcgttc atccatagct gcctgactcc ccgtcgtgta gataactacg
     2821 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2881 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2941 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3001 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3061 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3121 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3181 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3241 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3301 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3361 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3421 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3481 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3541 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3601 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3661 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3721 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3781 tttcgtcttc ac
//
