Basic Vector Information
- Vector Name:
- pNCO-HISACT-(C49S)-ASLOV1-syn
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4130 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Eisenreich W, Joshi M, Illarionov B, Richter G, Romisch-Margl W, Muller F, Bacher A, Fischer M.
- Promoter:
- T5
pNCO-HISACT-(C49S)-ASLOV1-syn vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNCO-HISACT-(C49S)-ASLOV1-syn vector Sequence
LOCUS 40924_33078 4130 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector pNCO-HISACT-(C49S)-ASLOV1-syn, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4130) AUTHORS Eisenreich W, Joshi M, Illarionov B, Richter G, Romisch-Margl W, Muller F, Bacher A, Fischer M. TITLE (13)C Isotopologue editing of FMN bound to phototropin domains JOURNAL FEBS J. 274 (22), 5876-5890 (2007) PUBMED 17944933 REFERENCE 2 (bases 1 to 4130) AUTHORS Fischer M. TITLE Direct Submission JOURNAL Submitted (22-FEB-2007) Chemistry, University of Hamburg, Grindelallee 117, Hamburg 20146, Germany REFERENCE 3 (bases 1 to 4130) TITLE Direct Submission REFERENCE 4 (bases 1 to 4130) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J."; date: "2007"; volume: "274"; issue: "22"; pages: "5876-5890" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (22-FEB-2007) Chemistry, University of Hamburg, Grindelallee 117, Hamburg 20146, Germany" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4130 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 10..54 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 62..78 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 97..108 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 115..468 /gene="hatA" /label=hatA /note="Hisactophilin-1 from Dictyostelium discoideum. Accession#: P13231" CDS 487..504 /label=thrombin site /note="thrombin recognition and cleavage site" misc_feature 505..849 /note="Region: Avena sativa phototropin PHY3 fusion protein Lov1 domain" terminator 872..966 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 1738..1824 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" misc_feature 1980..2120 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(2306..2894) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3068..3925) /label=AmpR /note="beta-lactamase" promoter complement(3926..4030) /label=AmpR promoter
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