LOCUS       V004346                 4130 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004346
VERSION     V004346
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Eisenreich W, Joshi M, Illarionov B, Richter G, Romisch-Margl W,
            Muller F, Bacher A, Fischer M.
  TITLE     (13)C Isotopologue editing of FMN bound to phototropin domains
  JOURNAL   FEBS J. 274 (22), 5876-5890 (2007)
   PUBMED   17944933
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Fischer M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2007) Chemistry, University of Hamburg,
            Grindelallee 117, Hamburg 20146, Germany
REFERENCE   3  (bases 1 to 4130)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J.";
            date: "2007"; volume: "274"; issue: "22"; pages: "5876-5890"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2007) Chemistry, University of Hamburg, Grindelallee 117,
            Hamburg 20146, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             115..468
                     /gene="hatA"
                     /label="Hisactophilin-1"
                     /note="Hisactophilin-1 from Dictyostelium discoideum.
                     Accession#: P13231"
     CDS             487..504
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_feature    505..849
                     /note="Region: Avena sativa phototropin PHY3 fusion protein
                     Lov1 domain"
     terminator      872..966
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      1738..1824
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1980..2120
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2306..2894)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3068..3925)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3926..4030)
                     /label="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatgggt
      121 aacagagcat tcaaatcaca tcacggtcac tttttaagcg ctgaaggcga agctgtaaag
      181 actcaccacg gtcatcatga tcatcacacc catttccacg ttgaaaacca tggtggtaaa
      241 gttgcattaa agacccattc cggtaaatac ctttcaattg gtgatcataa acaagtttac
      301 ctctcacacc acttacacgg tgaccactca ctcttccact tagaacatca tggcggtaaa
      361 gtctcaatca aaggtcatca ccaccactac atttccgctg atcatcatgg tcatgtttca
      421 accaaagaac accacgatca cgacaccacc tttgaagaaa ttattattgg ttccgcggca
      481 tctggtctgg tgccacgcgg atctgcactg tccgcattcc aacagacctt cgtagtttcg
      541 gacgccagcc gtccaggtca cccaatcatg tacgcaagcg ctggtttctt caacatgacc
      601 ggttacacat ccaaggaagt ggtaggtcgt aactgtcgtt tcctccaagg ttccggcacg
      661 gatccagctg agattgccaa gatccgtcag gctctggcaa atggttcgaa ctactgcggc
      721 cgtgtcctca actacaagaa agacggtacc gcattctgga atctcctgac cattgcacca
      781 atcaaggatg aagaaggtcg tgtccttaag ttcatcggca tgcaagtgga agtaagtaaa
      841 tacactgaat aagcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc
      901 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
      961 gagaatccaa gctagcttgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     1021 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     1081 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     1141 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     1201 ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat
     1261 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     1321 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     1381 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     1441 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     1501 caacttcttc gcccccgttt tcaccatgca tgggcaaata ttatacgcaa ggcgacaagg
     1561 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     1621 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt
     1681 aaggcagtta ttggtgccct taaacgcctg gggtaatgac tctctagctt gaggcatcaa
     1741 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1801 aacgctctcc tgagtaggac aaatccgccg ctctagagct gcctcgcgcg tttcggtgat
     1861 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     1921 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     1981 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     2041 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     2101 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2161 tctgtcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2221 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2281 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2341 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2401 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2461 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     2521 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2581 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2641 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2701 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2761 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2821 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2881 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2941 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3001 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3061 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3121 ccatagctgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3181 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3241 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3301 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3361 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3421 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3481 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3541 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3601 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3661 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3721 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3781 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3841 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3901 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3961 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4021 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4081 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac
//
