pNC-GFP vector (V004348)

LOCUS       40924_33058        6966 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNC-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Belbahri L, Champouret N, Mauch F.
  TITLE     Versatile cloning vectors for Phytophthora transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Belbahri L, Champouret N, Mauch F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2007) Laboratory of Applied Genetics, School of 
            Engineering of Lullier, 150 Route de Presinge, Jussy 1254, 
            Switzerland
REFERENCE   3  (bases 1 to 6966)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6966)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-OCT-2007) Laboratory of Applied Genetics, School of Engineering 
            of Lullier, 150 Route de Presinge, Jussy 1254, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     667..683
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(717..733)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    733..757
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      760..1323
                     /label=5' Bremia lactucae Ham34 promoter
                     /note="5' Bremia lactucae Ham34 promoter"
                     /regulatory_class="promoter"
     protein_bind    complement(1325..1349)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1371..2084
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     regulatory      2168..2644
                     /label=3' Bremia lactucae Ham34 terminator
                     /note="3' Bremia lactucae Ham34 terminator"
                     /regulatory_class="terminator"
     regulatory      2652..3235
                     /label=5' Bremia lactucae Hsp70 promoter
                     /note="5' Bremia lactucae Hsp70 promoter"
                     /regulatory_class="promoter"
     CDS             3251..4042
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      4232..4451
                     /label=3' Bremia lactucae Hsp70 terminator
                     /note="3' Bremia lactucae Hsp70 terminator"
                     /regulatory_class="terminator"
     promoter        complement(4778..4796)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4817..4833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4841..4857)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4865..4895)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4910..4931)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5219..5807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5981..6838)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6839..6943)
                     /label=AmpR promoter