pNC-GFP vector (V004348)

MSDS

Basic Vector Information

Vector Name:: pNC-GFP

Antibiotic Resistance:: Ampicillin

Length:: 6966 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Belbahri L, Champouret N, Mauch F.

pNC-GFP vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNC-GFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33058        6966 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNC-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Belbahri L, Champouret N, Mauch F.
  TITLE     Versatile cloning vectors for Phytophthora transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Belbahri L, Champouret N, Mauch F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2007) Laboratory of Applied Genetics, School of 
            Engineering of Lullier, 150 Route de Presinge, Jussy 1254, 
            Switzerland
REFERENCE   3  (bases 1 to 6966)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6966)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-OCT-2007) Laboratory of Applied Genetics, School of Engineering 
            of Lullier, 150 Route de Presinge, Jussy 1254, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     667..683
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(717..733)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    733..757
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      760..1323
                     /label=5' Bremia lactucae Ham34 promoter
                     /note="5' Bremia lactucae Ham34 promoter"
                     /regulatory_class="promoter"
     protein_bind    complement(1325..1349)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1371..2084
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     regulatory      2168..2644
                     /label=3' Bremia lactucae Ham34 terminator
                     /note="3' Bremia lactucae Ham34 terminator"
                     /regulatory_class="terminator"
     regulatory      2652..3235
                     /label=5' Bremia lactucae Hsp70 promoter
                     /note="5' Bremia lactucae Hsp70 promoter"
                     /regulatory_class="promoter"
     CDS             3251..4042
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      4232..4451
                     /label=3' Bremia lactucae Hsp70 terminator
                     /note="3' Bremia lactucae Hsp70 terminator"
                     /regulatory_class="terminator"
     promoter        complement(4778..4796)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4817..4833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4841..4857)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4865..4895)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4910..4931)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5219..5807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5981..6838)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6839..6943)
                     /label=AmpR promoter

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