LOCUS       40924_33058        6966 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNC-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Belbahri L, Champouret N, Mauch F.
  TITLE     Versatile cloning vectors for Phytophthora transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Belbahri L, Champouret N, Mauch F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2007) Laboratory of Applied Genetics, School of 
            Engineering of Lullier, 150 Route de Presinge, Jussy 1254, 
            Switzerland
REFERENCE   3  (bases 1 to 6966)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6966)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-OCT-2007) Laboratory of Applied Genetics, School of Engineering 
            of Lullier, 150 Route de Presinge, Jussy 1254, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     667..683
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(717..733)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    733..757
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      760..1323
                     /label=5' Bremia lactucae Ham34 promoter
                     /note="5' Bremia lactucae Ham34 promoter"
                     /regulatory_class="promoter"
     protein_bind    complement(1325..1349)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1371..2084
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     regulatory      2168..2644
                     /label=3' Bremia lactucae Ham34 terminator
                     /note="3' Bremia lactucae Ham34 terminator"
                     /regulatory_class="terminator"
     regulatory      2652..3235
                     /label=5' Bremia lactucae Hsp70 promoter
                     /note="5' Bremia lactucae Hsp70 promoter"
                     /regulatory_class="promoter"
     CDS             3251..4042
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      4232..4451
                     /label=3' Bremia lactucae Hsp70 terminator
                     /note="3' Bremia lactucae Hsp70 terminator"
                     /regulatory_class="terminator"
     promoter        complement(4778..4796)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4817..4833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4841..4857)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4865..4895)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4910..4931)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5219..5807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5981..6838)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6839..6943)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc
      661 ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc
      721 cactagttct agacaagttt gtacaaaaaa gcaggcttct ctgatggaca aagggtcgcc
      781 tctacgtacc cgcgacaatc tcggcttttc caatgtcgac agctggcata tcgcacgcga
      841 ggttaggcgt cttcagccgg cgtaaccaca aacctcgagt caagtaccct ttttcagacc
      901 ttaatttccg tgtccattcc ctcgcgttta cacgaaggtt tctgagaaat accacatgtc
      961 acagagagag agaagattgg agaaccttct caacctgacc cgtgcagagc gcttttgcgt
     1021 cctaccatcc gttaattata gcaccaccac cacatgtctc gccggaactc tcttatccgc
     1081 tctttttgat cgtctttttt gcgccccatt aaagacatct cttgcacagg gatcgtttct
     1141 gattggccat ttcacttcct cttttacatc cgaaaaccac ctgtttgtgg ggttgtgaag
     1201 ccacaaccgg acgctcaaaa agcctcgccc gactcgccca cgatcggaag gctcattctc
     1261 cttttcactc tcacgtgccc aagtcccaac cgactctttt tcgaccttca ctctcaccga
     1321 caagacccag ctttcttgta caaagtggtc tagagcggcc gccaccgcgg atgggcaagg
     1381 gcgaggaact gttcactggc gtggtcccaa tcctggtgga actggatggt gatgtgaacg
     1441 ggcacaagtt ctccgtcagc ggagagggtg aaggtgatgc cacctacgga aagctcaccc
     1501 tgaagttcat ctgcactacc ggaaagctcc ctgttccgtg gccaaccctc gtcaccactt
     1561 tcacctacgg tgttcagtgc ttctcccggt acccagatca catgaagcag catgacttct
     1621 tcaagagcgc catgcccgaa ggctacgtgc aagaaaggac tatcttcttc aaggatgacg
     1681 ggaactacaa gacacgtgcc gaagtcaagt tcgaaggtga taccctggtg aaccgcatcg
     1741 agctgaaagg catcgatttc aaggaagatg gaaacatcct cggacacaag ctggagtaca
     1801 actacaactc ccacaacgta tacatcatgg ccgacaagca gaagaacggc atcaaggtga
     1861 acttcaagat caggcacaac atcgaagatg gaagcgtgca actggcggac cactaccagc
     1921 agaacacgcc catcggcgat ggccctgtcc tgctgccgga caaccattac ctgtccacgc
     1981 aatctgccct ctccaaggac cccaacgaga agagggacca catggtcctg ctggagttcg
     2041 tgacggctgc tgggatcacg catggcatgg atgaactcta caagtgaaga tctgaattgg
     2101 gtaccggtgc ctacttcttg taagccgaag gtcgagctgt gtgcactgag tgcgaagttg
     2161 cagattttgt gtctatgcat ttaatacaat ttcctttttt ccattttcgt tgtttttttt
     2221 tgtgaaagaa cgattgtttt caaaagaagg ccgtttgtag aacaattttt ttttatacta
     2281 gcatttggta ttcgagtgtg acgaatcggt gatggcgctt tacaaatgaa atataacgct
     2341 gaacaagcgc agtgacagct ttattttata acagagaata aagaccaaag tagagtagac
     2401 tgatggcggc ttgaaacaaa actacgtgac ttctaatgca aagaaaacca taccccacgc
     2461 tacttagaat tgtttctctt tggttgatga ccgctatagt cgtatggagt gatccctttt
     2521 tgtcaatatt tcgattcttg ctaaaaccgt gactattttg aggcaactgt gtgtacttaa
     2581 catggtagaa atttctatgt ttaagccagc tgacaatcat tctttattta tcgtaaacca
     2641 accaccatat gcgtcaatgc aattgactgt gtcctcgatt atttctatta ttaatattat
     2701 ttccattaaa aaagaaatcc agttcgtggc aaaataagac aaaggtttaa ataaaaattt
     2761 atcgttatta aactcgtaac ttaccttctt catggctcaa cccaatctgt atatcgactt
     2821 atttaatttg gttaacaaat cggttttcgt cgcaaatagg ggatgacaac taaaatacca
     2881 atttgtgtcc ttgcgacacg tcaacaggtg gctgccaata tctcaggttt tgagcacaca
     2941 acgctgtgat tggctatctg tagatcgctt cgagaacttt cggaccgaca tatcagaacg
     3001 cagtcatgtc caatccagct aacgattggt cgaaaccgca cttcatcgct tcatttcaac
     3061 caattcccat ctactaaaac gagcgtcgga atagtctcga acgtaacccc acgtagtggg
     3121 tggcagcgcc cctcgtatct ttcaatcgcc actcgagtct caagtttgct cactttgaaa
     3181 ttttccatct ggactacaca tcaaatctaa ctcaactttt atcaattgaa acacaagggc
     3241 cccgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     3301 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     3361 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     3421 gaatgaactg caggacgagc cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     3481 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     3541 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     3601 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     3661 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     3721 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     3781 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     3841 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     3901 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     3961 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4021 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     4081 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4141 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     4201 gagttcttcg cccacccccc ggatccccgg ggtcgactag aatacttagc aattggttag
     4261 tttgaaaatt gtcaaagcag agttttttcg acaataaaat ttattttcta aatatttgat
     4321 ttagcgttga cttaatttgt tgattcggtt gtcatctttt gtgaagatgc ccgagagtct
     4381 agtaaatctc aagcgaaaca tagtgagaag gtcactatgg ctggtttgca tgagtctggg
     4441 cctattgtgc ttacgccgcc ttagtaacaa acatgccaaa agaaggaaag ctttcaccag
     4501 ctttcaagtt taatcaatct ttgagagtca caattgcaag aataaatgaa tttttacaac
     4561 acagttcgat gcactgtgtt tcgtcttgat gaaaattgga cggcaaatcg agtatcttaa
     4621 acaaatctcg gcataggctg tccattttgt ggtcgcctct ttgttggtag tttgcagtag
     4681 tttcattagt agaagatctg catcctcaat ctgctaaacg tcttgagcag ctatgagacg
     4741 atccggggaa ttggggcccg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     4801 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4861 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     4921 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     4981 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5041 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5101 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5161 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5221 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5281 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5341 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5401 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5461 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5521 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5581 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5641 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5701 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5761 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5821 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5881 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5941 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6001 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6061 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6121 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6181 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6241 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6301 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6361 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6421 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6481 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6541 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6601 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6661 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6721 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6781 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6841 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6901 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6961 tgccac
//
