pNB97 vector (V004351)

LOCUS       40924_33043        9151 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNB97, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Bongio NJ, Lampe DJ.
  TITLE     Inhibition of Plasmodium berghei Development in Mosquitoes by 
            Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel 
            Native Secretion Signal
  JOURNAL   PLoS ONE 10 (12), E0143541 (2015)
  PUBMED    26636338
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   Bongio NJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2015) Biology, Shenandoah University, 1460 
            University Drive, Winchester, VA 22601, USA
REFERENCE   3  (bases 1 to 9151)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9151)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "12"; pages: "E0143541"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-SEP-2015) Biology, Shenandoah University, 1460 University Drive,
            Winchester, VA 22601, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1762
                     /label=nptII
                     /note="nptII"
                     /regulatory_class="promoter"
     CDS             3492..3521
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3534..3758
                     /codon_start=1
                     /product="scorpine"
                     /label=scorpine
                     /protein_id="ALR84561.1"
                     /translation="GWINEEKIQKKIDERMGNTVLGGMAKAIVHKMAKNEFQCMANMDM
                     LGNCEKHCQTSGEKGYCHGTKCKCGTPLSY"
     CDS             3771..5108
                     /codon_start=1
                     /product="alkaline phosphatase"
                     /label=alkaline phosphatase
                     /protein_id="ALR84562.1"
                     /translation="PVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLI
                     GDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATA
                     WSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRK
                     CYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLR
                     EQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVT
                     CTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVD
                     LDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYG
                     NSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK"
     CDS             complement(6700..7359)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(7360..8129)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"