LOCUS       40924_33043        9151 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNB97, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Bongio NJ, Lampe DJ.
  TITLE     Inhibition of Plasmodium berghei Development in Mosquitoes by 
            Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel 
            Native Secretion Signal
  JOURNAL   PLoS ONE 10 (12), E0143541 (2015)
  PUBMED    26636338
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   Bongio NJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2015) Biology, Shenandoah University, 1460 
            University Drive, Winchester, VA 22601, USA
REFERENCE   3  (bases 1 to 9151)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9151)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "12"; pages: "E0143541"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-SEP-2015) Biology, Shenandoah University, 1460 University Drive,
            Winchester, VA 22601, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1762
                     /label=nptII
                     /note="nptII"
                     /regulatory_class="promoter"
     CDS             3492..3521
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3534..3758
                     /codon_start=1
                     /product="scorpine"
                     /label=scorpine
                     /protein_id="ALR84561.1"
                     /translation="GWINEEKIQKKIDERMGNTVLGGMAKAIVHKMAKNEFQCMANMDM
                     LGNCEKHCQTSGEKGYCHGTKCKCGTPLSY"
     CDS             3771..5108
                     /codon_start=1
                     /product="alkaline phosphatase"
                     /label=alkaline phosphatase
                     /protein_id="ALR84562.1"
                     /translation="PVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLI
                     GDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATA
                     WSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRK
                     CYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLR
                     EQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVT
                     CTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVD
                     LDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYG
                     NSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK"
     CDS             complement(6700..7359)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(7360..8129)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca taaccggaat
     1561 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     1621 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgtc
     1681 gactctagag gatccccggg taccgagctc gaattcggct tattccctaa ctaactaaag
     1741 attaacttta taaggaggaa aaacatatgc ccagagccgc tcgacgcgtg ccagggctgt
     1801 ttccgcaccg gttgggggac gctcctgcga cctcgcgccc cgaaaccatc atggtgacgg
     1861 gctcgctgtt ccgcgatccg aacacgacca gtgcgtcacc gattacgcac atcaccatga
     1921 aggacatgca gcagcgtggc atcaagaccg tgaccgatgc gctgcagctc ctgtcttcca
     1981 acggtgcagg taatctgacc aatgcctggt cggcgggtgg tggctttgcc gccggtgcat
     2041 cggctccgtc gctccgcggt ctcagcacgg attcgacgct ggtcctcatg gatgggcagc
     2101 gcctgtcgta ctatccgctg gccgatgacg gcgagcgcaa ctttgtcgac accaactgga
     2161 tgccgcaatc catcatgcag ggcgtggacg tgctgcagga tggcggctcg gcaacctatg
     2221 gtgctgacgc cgttgccggt gtggtcaaca tgatcacccg taaggaaatc aagggtttcg
     2281 agggtaacgc cgaaggtggc ctgacgcagc gtggcgatgc ggggcatcag cgcctctacg
     2341 ccacctgggg tcatggcgac ctgcacaagg acggctataa cttctacatc aactcggaat
     2401 atcagcagga cgatccgctt tattaccgtc agctcaattc accgtataac aatggtgacc
     2461 tgaccgggat cggtggcagc aacggtaacg ataacatcgt ctcgggtggc tcgatcacca
     2521 atttcgcgcc gtcgaccgta gcgctcgtac gtcctgtaac gggaaccggc accaatacgg
     2581 caccctggtc gcttctgaac cagcaggcgg gttgccgtgg cctcgatggc ctggttacag
     2641 gaactgttgg cggaagcacc ggcataagcc aggcctgctc gtggaataca cagaaatacg
     2701 cccaggtcgc gcctgacctg cgtcgcgtca gtgccacggc tcacttcacg gctaatgtca
     2761 ccgagcgttc acagctgaca gccatgttca cctggtcgca ggcgcgttcc atcatcaccg
     2821 gtacgcccta ctcgacctat ggcggcacgt cctacagcca ggcaaagctg gcgacagcgc
     2881 agaacaccta tctgcctgtc tatctggctg atggctcgct caacccgaac aacccttacg
     2941 ccgcacaggg tgagcaggct caggtcaatt accgtttcgg cgatctgatt ccgaccacca
     3001 tggacacgag ccagaacttc cgtggctcgg tgcgctacaa tggctggcat ccgtcgcgct
     3061 ggggttcaga ctggaattac gacgtcaact tcgttggcat gaacacgctg ctgaaccaga
     3121 cgatcacggg cgtgccgacc atctccggca tccagaacgc cattaccagc ggcagctaca
     3181 atttcgtgga tccgtcgcag aactcgcagt cggtgctgaa cgccatcgcg ccgcgtaatg
     3241 tcatcaatgc gcgcacacag gaatattcag gcgaagccac gctcagcaag ggtctgttcc
     3301 acctgccggg tggtatggcc aagctcgcca tcggcggcaa cattcgttac gaagccctga
     3361 acgatccgaa cgccaacccg ctcgatacgg ccaatccggc caaccagtac accagctata
     3421 tcaatcctgt gaatgctcag ggcagccgct gggtcgagtc aggttttttc caggctgagg
     3481 cgccgattgt tgaacagaaa ctgatctcgg aggaagatct gaacttaatt aaaggctgga
     3541 ttaacgaaga gaagattcag aaaaagattg acgaacggat gggcaacacg gtcctgggtg
     3601 gcatggcgaa agcgattgtg cataaaatgg ccaagaacga atttcagtgc atggcgaaca
     3661 tggatatgct gggcaattgc gagaaacact gtcagacgag cggcgaaaaa ggctactgtc
     3721 acggcaccaa gtgtaaatgc ggcacccccc tgtcttattc ctgcaggatg cctgttctgg
     3781 aaaaccgggc tgctcagggc gatattactg cacccggcgg tgctcgccgt ttaacgggtg
     3841 atcagactgc cgctctgcgt gattctctta gcgataaacc tgcaaaaaat attattttgc
     3901 tgattggcga tgggatgggg gactcggaaa ttactgccgc acgtaattat gccgaaggtg
     3961 cgggcggctt ttttaaaggt atagatgcct taccgcttac cgggcaatac actcactatg
     4021 cgctgaataa aaaaaccggc aaaccggact acgtcaccga ctcggctgca tcagcaaccg
     4081 cctggtcaac cggtgtcaaa acctataacg gcgcgctggg cgtcgatatt cacgaaaaag
     4141 atcacccaac gattctggaa atggcaaaag ccgcaggtct ggcgaccggt aacgtttcta
     4201 ccgcagagtt gcaggatgcc acgcccgctg cgctggtggc acatgtgacc tcgcgcaaat
     4261 gctacggtcc gagcgcgacc agtgaaaaat gtccgggtaa cgctctggaa aaaggcggaa
     4321 aaggatcgat taccgaacag ctgcttaacg ctcgtgccga cgttacgctt ggcggcggcg
     4381 caaaaacctt tgctgaaacg gcaaccgctg gtgaatggca gggaaaaacg ctgcgtgaac
     4441 aggcacaggc gcgtggttat cagttggtga gcgatgctgc ctcactgaat tcggtgacgg
     4501 aagcgaatca gcaaaaaccc ctgcttggcc tgtttgctga cggcaatatg ccagtgcgct
     4561 ggctaggacc gaaagcaacg taccatggca atatcgataa gcccgcagtc acctgtacgc
     4621 caaatccgca acgtaatgac agtgtaccaa ccctggcgca gatgaccgac aaagccattg
     4681 aattgttgag taaaaatgag aaaggctttt tcctgcaagt tgaaggtgcg tcaatcgata
     4741 aacaggatca tgctgcgaat ccttgtgggc aaattggcga gacggtcgat ctcgatgaag
     4801 ccgtacaacg ggcgctggaa ttcgctaaaa aggagggtaa cacgctggtc atagtcaccg
     4861 ctgatcacgc ccacgccagc cagattgttg cgccggatac caaagctccg ggcctcaccc
     4921 aggcgctaaa taccaaagat ggcgcagtga tggtgatgag ttacgggaac tccgaagagg
     4981 attcacaaga acataccggc agtcagttgc gtattgcggc gtatggcccg catgccgcca
     5041 atgttgttgg actgaccgac cagaccgatc tcttctacac catgaaagcc gctctggggc
     5101 tgaaataaaa ccgcgcccgg cagtgaattt tcgctgccgg gtggtttttt tgctgttagc
     5161 aaccagactt aatggcagat cacgggcgca tacgctcatg gttaaaacat gaagagggat
     5221 ggtgctatga aaataacatt actggttacc ttgcttttcg gtctggtttt tttaaccacc
     5281 gtcggcgctg ccgagagaac tttaacccca caacaacagc gtatgacctc ctgtaatcag
     5341 caggcgacgg cgcaggcgtt gaaaggggat gctcgtaaga cctacatgag tgattgcctg
     5401 aagaacagca agtctgcgcc tggcgaaaaa agtttgacgc cacagcagca aaagatgcgc
     5461 gaatgcaata atcaagcaac acaacaatct ctgaaaggtg atgatcgtaa taagtttatg
     5521 agtgcctgcc tcaagaaagc cgcctgatac ctgatagtgc taacgggtga gctacgaaaa
     5581 tggctcaccc gaaatatcat acttctgcct ttagctccgt ctctataatt tgggaaaatt
     5641 gtttctgaat gttcccaaaa ataatgaatg atgaaaactt tttcaaaaaa gcggcggcgc
     5701 acggggagga acctccttta actcctcaaa acgaacatca gcggtccggg ctgcgcttcg
     5761 cccgtcgcgt cagactaccc cgtgcggttg gcctggctgg catgttctta ccgattgctt
     5821 caacgctggt ttcacacccg ccgccgggct ggtggtggct ggtgttggtc ggctgggcgt
     5881 tcgtctggcc gcatttagcc tggcagatag cgagcagggc cgtcgatccg cttagccggg
     5941 aaatttacaa cttaaaaacc gatgcagtat tagcgggaat gtgggtaggc gtaatgggcg
     6001 taaacgtgct gccttccacc gcgatgttga tgattatgtg tctgaatttg atgggggcag
     6061 gcggcccccg tctgtttgtc gcgggtctgg tgttgatggt ggtttcctgc cttgtcaccg
     6121 gccggccgga tccgaattcg cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     6181 atcagctcat tttttaacca ataggccgac tgcgatgagt ggcagggcgg ggcgtaattt
     6241 ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata agtgataata
     6301 agcggatgaa tggcagaaat tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg
     6361 tcgttaaata gccgcttatg tctattgctg gtttaccggt ttattgacta ccggaagcag
     6421 tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct caggctctcc ccgtggaggt
     6481 aataattgac gatatgatca tttattctgc ctcccagagc ctgataaaaa cggtgaatcc
     6541 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga
     6601 cgcaacgcgg ggaggcagac aaggtatagg gcggcgaggc ggctacagcc gatagtctgg
     6661 aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca gcgtgacccg
     6721 tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag
     6781 gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc
     6841 catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg
     6901 ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc
     6961 ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg
     7021 gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt
     7081 gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt
     7141 ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg
     7201 gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat
     7261 aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt
     7321 cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg
     7381 tgccgcacgg ttgcggcacc atgcgcaatc agctgcaact tttcggcagc gcgacaacaa
     7441 ttatgcgttg cgtaaaagtg gcagtcaatt acagattttc tttaacctac gcaatgagct
     7501 attgcggggg gtgccgcaat gagctgttgc gtacccccct tttttaagtt gttgattttt
     7561 aagtctttcg catttcgccc tatatctagt tctttggtgc ccaaagaagg gcacccctgc
     7621 ggggttcccc cacgccttcg gcgcggctcc ccctccggca aaaagtggcc cctccggggc
     7681 ttgttgatcg actgcgcggc cttcggcctt gcccaaggtg gcgctgcccc cttggaaccc
     7741 ccgcactcgc cgccgtgagg ctcggggggc aggcgggcgg gcttcgcctt cgactgcccc
     7801 cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg
     7861 cgcgagcctt gacccgcctt ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc
     7921 aggccggagg cttttcccca gagaaaatta aaaaaattga tggggcaagg ccgcaggccg
     7981 cgcagttgga gccggtgggt atgtggtcga aggctgggta gccggtgggc aatccctgtg
     8041 gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt tgtccacggg
     8101 ccgagcgaag cgagccagcc ggtggccgct cgcggccatc gtccacatat ccacgggctg
     8161 gcaagggagc gcagcgaccg cgcagggcga agcccggaga gcaagcccgt agggcgccgc
     8221 agccgccgta ggcggtcacg actttgcgaa gcaaagtcta gtgagtatac tcaagcattg
     8281 agtggcccgc cggaggcacc gccttgcgct gcccccgtcg agccggttgg acaccaaaag
     8341 ggaggggcag gcatggcggc atacgcgatc atgcgatgca agaagctggc gaaaatgggc
     8401 aacgtggcgg ccagtctcaa gcacgcctac cgcgagcgcg agacgcccaa cgctgacgcc
     8461 agcaggacgc cagagaacga gcactgggcg gccagcagca ccgatgaagc gatgggccga
     8521 ctgcgcgagt tgctgccaga gaagcggcgc aaggacgctg tgttggcggt cgagtacgtc
     8581 atgacggcca gcccggaatg gtggaagtcg gccagccaag aacagcaggc ggcgttcttc
     8641 gagaaggcgc acaagtggct ggcggacaag tacggggcgg atcgcatcgt gacggccagc
     8701 atccaccgtg acgaaaccag cccgcacatg accgcgttcg tggtgccgct gacgcaggac
     8761 ggcaggctgt cggccaagga gttcatcggc aacaaagcgc agatgacccg cgaccagacc
     8821 acgtttgcgg ccgctgtggc cgatctaggg ctgcaacggg gcatcgaggg cagcaaggca
     8881 cgtcacacgc gcattcaggc gttctacgag gccctggagc ggccaccagt gggccacgtc
     8941 accatcagcc cgcaagcggt cgagccacgc gcctatgcac cgcagggatt ggccgaaaag
     9001 ctgggaatct caaagcgcgt tgagacgccg gaagccgtgg ccgaccggct gacaaaagcg
     9061 gttcggcagg ggtatgagcc tgccctacag gccgccgcag gagcgcgtga gatgcgcaag
     9121 aaggccgatc aagcccaaga gacggcccga g
//
