pNB95 vector (V004352)

LOCUS       V004352                 9154 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004352
VERSION     V004352
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Bongio NJ, Lampe DJ.
  TITLE     Inhibition of Plasmodium berghei Development in Mosquitoes by
            Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel
            Native Secretion Signal
  JOURNAL   PLoS ONE 10 (12), E0143541 (2015)
   PUBMED   26636338
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Bongio NJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2015) Biology, Shenandoah University, 1460
            University Drive, Winchester, VA 22601, USA
REFERENCE   3  (bases 1 to 9154)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9154)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2015"; volume: "10"; issue: "12"; pages: "E0143541"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-SEP-2015) Biology, Shenandoah University, 1460 University Drive,
            Winchester, VA 22601, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1762
                     /label="nptII"
                     /note="nptII"
                     /regulatory_class="promoter"
     CDS             1767..3524
                     /codon_start=1
                     /product="TonB dependent siderophore receptor"
                     /label="TonB dependent siderophore receptor"
                     /protein_id="ALR84557.1"
                     /translation="MTLLPRETHDSGRHNPIVAPSTRRKRPLAAGLMSATACVALLHFV
                     SDARAQSVSASETSTIPVNAAKAPAKSKVKVNAQSRSTRARAVSAPVGDAPATSRPETI
                     MVTGSLFRDPNTTSASPITHITMKDMQQRGIKTVTDALQLLSSNGAGNLTNAWSAGGGF
                     AAGASAPSLRGLSTDSTLVLMDGQRLSYYPLADDGERNFVDTNWMPQSIMQGVDVLQDG
                     GSATYGADAVAGVVNMITRKEIKGFEGNAEGGLTQRGDAGHQRLYATWGHGDLHKDGYN
                     FYINSEYQQDDPLYYRQLNSPYNNGDLTGIGGSNGNDNIVSGGSITNFAPSTVALVRPV
                     TGTGTNTAPWSLLNQQAGCRGLDGLVTGTVGGSTGISQACSWNTQKYAQVAPDLRRVSA
                     TAHFTANVTERSQLTAMFTWSQARSIITGTPYSTYGGTSYSQAKLATAQNTYLPVYLAD
                     GSLNPNNPYAAQGEQAQVNYRFGDLIPTTMDTSQNFRGSVRYNGWHPSRWGSDWNYDVN
                     FVGMNTLLNQTITGVPTISGIQNAITSGSYNFVDPSQNSQSVLNAIAPRNVINARTQEY
                     SGEATLSKGL"
     CDS             3720..3749
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3759..5108
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     CDS             complement(6703..7362)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(7363..8132)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"