Basic Vector Information
- Vector Name:
- pNB95
- Antibiotic Resistance:
- Kanamycin
- Length:
- 9154 bp
- Type:
- Cloning vector
- Replication origin:
- pBBR1 oriV
- Source/Author:
- Bongio NJ, Lampe DJ.
pNB95 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNB95 vector Sequence
LOCUS 40924_33038 9154 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNB95, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9154)
AUTHORS Bongio NJ, Lampe DJ.
TITLE Inhibition of Plasmodium berghei Development in Mosquitoes by
Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel
Native Secretion Signal
JOURNAL PLoS ONE 10 (12), E0143541 (2015)
PUBMED 26636338
REFERENCE 2 (bases 1 to 9154)
AUTHORS Bongio NJ.
TITLE Direct Submission
JOURNAL Submitted (13-SEP-2015) Biology, Shenandoah University, 1460
University Drive, Winchester, VA 22601, USA
REFERENCE 3 (bases 1 to 9154)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9154)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2015"; volume: "10"; issue: "12"; pages: "E0143541"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-SEP-2015) Biology, Shenandoah University, 1460 University Drive,
Winchester, VA 22601, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9154
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 458..1249
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
regulatory 1552..1762
/label=nptII
/note="nptII"
/regulatory_class="promoter"
CDS 1767..3524
/codon_start=1
/product="TonB dependent siderophore receptor"
/label=TonB dependent siderophore receptor
/protein_id="ALR84557.1"
/translation="MTLLPRETHDSGRHNPIVAPSTRRKRPLAAGLMSATACVALLHFV
SDARAQSVSASETSTIPVNAAKAPAKSKVKVNAQSRSTRARAVSAPVGDAPATSRPETI
MVTGSLFRDPNTTSASPITHITMKDMQQRGIKTVTDALQLLSSNGAGNLTNAWSAGGGF
AAGASAPSLRGLSTDSTLVLMDGQRLSYYPLADDGERNFVDTNWMPQSIMQGVDVLQDG
GSATYGADAVAGVVNMITRKEIKGFEGNAEGGLTQRGDAGHQRLYATWGHGDLHKDGYN
FYINSEYQQDDPLYYRQLNSPYNNGDLTGIGGSNGNDNIVSGGSITNFAPSTVALVRPV
TGTGTNTAPWSLLNQQAGCRGLDGLVTGTVGGSTGISQACSWNTQKYAQVAPDLRRVSA
TAHFTANVTERSQLTAMFTWSQARSIITGTPYSTYGGTSYSQAKLATAQNTYLPVYLAD
GSLNPNNPYAAQGEQAQVNYRFGDLIPTTMDTSQNFRGSVRYNGWHPSRWGSDWNYDVN
FVGMNTLLNQTITGVPTISGIQNAITSGSYNFVDPSQNSQSVLNAIAPRNVINARTQEY
SGEATLSKGL"
CDS 3720..3749
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
CDS 3759..5108
/gene="phoA"
/label=phoA
/note="Alkaline phosphatase from Escherichia coli (strain
K12). Accession#: P00634"
CDS complement(6703..7362)
/label=pBBR1 Rep
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
rep_origin complement(7363..8132)
/direction=LEFT
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
This page is informational only.