LOCUS       V004352                 9154 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004352
VERSION     V004352
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Bongio NJ, Lampe DJ.
  TITLE     Inhibition of Plasmodium berghei Development in Mosquitoes by
            Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel
            Native Secretion Signal
  JOURNAL   PLoS ONE 10 (12), E0143541 (2015)
   PUBMED   26636338
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Bongio NJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2015) Biology, Shenandoah University, 1460
            University Drive, Winchester, VA 22601, USA
REFERENCE   3  (bases 1 to 9154)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9154)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2015"; volume: "10"; issue: "12"; pages: "E0143541"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-SEP-2015) Biology, Shenandoah University, 1460 University Drive,
            Winchester, VA 22601, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1762
                     /label="nptII"
                     /note="nptII"
                     /regulatory_class="promoter"
     CDS             1767..3524
                     /codon_start=1
                     /product="TonB dependent siderophore receptor"
                     /label="TonB dependent siderophore receptor"
                     /protein_id="ALR84557.1"
                     /translation="MTLLPRETHDSGRHNPIVAPSTRRKRPLAAGLMSATACVALLHFV
                     SDARAQSVSASETSTIPVNAAKAPAKSKVKVNAQSRSTRARAVSAPVGDAPATSRPETI
                     MVTGSLFRDPNTTSASPITHITMKDMQQRGIKTVTDALQLLSSNGAGNLTNAWSAGGGF
                     AAGASAPSLRGLSTDSTLVLMDGQRLSYYPLADDGERNFVDTNWMPQSIMQGVDVLQDG
                     GSATYGADAVAGVVNMITRKEIKGFEGNAEGGLTQRGDAGHQRLYATWGHGDLHKDGYN
                     FYINSEYQQDDPLYYRQLNSPYNNGDLTGIGGSNGNDNIVSGGSITNFAPSTVALVRPV
                     TGTGTNTAPWSLLNQQAGCRGLDGLVTGTVGGSTGISQACSWNTQKYAQVAPDLRRVSA
                     TAHFTANVTERSQLTAMFTWSQARSIITGTPYSTYGGTSYSQAKLATAQNTYLPVYLAD
                     GSLNPNNPYAAQGEQAQVNYRFGDLIPTTMDTSQNFRGSVRYNGWHPSRWGSDWNYDVN
                     FVGMNTLLNQTITGVPTISGIQNAITSGSYNFVDPSQNSQSVLNAIAPRNVINARTQEY
                     SGEATLSKGL"
     CDS             3720..3749
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3759..5108
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     CDS             complement(6703..7362)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(7363..8132)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca taaccggaat
     1561 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     1621 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgtc
     1681 gactctagag gatccccggg taccgagctc gaattcggct tattccctaa ctaactaaag
     1741 attaacttta taaggaggaa aaacatatga cgctgctccc aagagagacg cacgattcgg
     1801 gtcgccataa cccgatcgtc gccccgtcaa cccgacgcaa gcgaccgctt gccgccggtc
     1861 tcatgagtgc cacagcctgt gtcgctctgt tgcatttcgt cagtgacgca cgggcacaga
     1921 gtgtttcggc ctctgaaaca tcgacaatcc ctgtcaatgc tgccaaggct cctgccaaga
     1981 gcaaggtgaa ggtcaatgcc cagagccgct cgacgcgtgc cagggctgtt tccgcaccgg
     2041 ttggggacgc tcctgcgacc tcgcgccccg aaaccatcat ggtgacgggc tcgctgttcc
     2101 gcgatccgaa cacgaccagt gcgtcaccga ttacgcacat caccatgaag gacatgcagc
     2161 agcgtggcat caagaccgtg accgatgcgc tgcagctcct gtcttccaac ggtgcaggta
     2221 atctgaccaa tgcctggtcg gcgggtggtg gctttgccgc cggtgcatcg gctccgtcgc
     2281 tccgcggtct cagcacggat tcgacgctgg tcctcatgga tgggcagcgc ctgtcgtact
     2341 atccgctggc cgatgacggc gagcgcaact ttgtcgacac caactggatg ccgcaatcca
     2401 tcatgcaggg cgtggacgtg ctgcaggatg gcggctcggc aacctatggt gctgacgccg
     2461 ttgccggtgt ggtcaacatg atcacccgta aggaaatcaa gggtttcgag ggtaacgccg
     2521 aaggtggcct gacgcagcgt ggcgatgcgg ggcatcagcg cctctacgcc acctggggtc
     2581 atggcgacct gcacaaggac ggctataact tctacatcaa ctcggaatat cagcaggacg
     2641 atccgcttta ttaccgtcag ctcaattcac cgtataacaa tggtgacctg accgggatcg
     2701 gtggcagcaa cggtaacgat aacatcgtct cgggtggctc gatcaccaat ttcgcgccgt
     2761 cgaccgtagc gctcgtacgt cctgtaacgg gaaccggcac caatacggca ccctggtcgc
     2821 ttctgaacca gcaggcgggt tgccgtggcc tcgatggcct ggttacagga actgttggcg
     2881 gaagcaccgg cataagccag gcctgctcgt ggaatacaca gaaatacgcc caggtcgcgc
     2941 ctgacctgcg tcgcgtcagt gccacggctc acttcacggc taatgtcacc gagcgttcac
     3001 agctgacagc catgttcacc tggtcgcagg cgcgttccat catcaccggt acgccctact
     3061 cgacctatgg cggcacgtcc tacagccagg caaagctggc gacagcgcag aacacctatc
     3121 tgcctgtcta tctggctgat ggctcgctca acccgaacaa cccttacgcc gcacagggtg
     3181 agcaggctca ggtcaattac cgtttcggcg atctgattcc gaccaccatg gacacgagcc
     3241 agaacttccg tggctcggtg cgctacaatg gctggcatcc gtcgcgctgg ggttcagact
     3301 ggaattacga cgtcaacttc gttggcatga acacgctgct gaaccagacg atcacgggcg
     3361 tgccgaccat ctccggcatc cagaacgcca ttaccagcgg cagctacaat ttcgtggatc
     3421 cgtcgcagaa ctcgcagtcg gtgctgaacg ccatcgcgcc gcgtaatgtc atcaatgcgc
     3481 gcacacagga atattcaggc gaagccacgc tcagcaaggg tctgttccac ctgccgggtg
     3541 gtatggccaa gctcgccatc ggcggcaaca ttcgttacga agccctgaac gatccgaacg
     3601 ccaacccgct cgatacggcc aatccggcca accagtacac cagctatatc aatcctgtga
     3661 atgctcaggg cagccgctgg gtcgagtcag gttttttcca ggctgaggcg ccgattgttg
     3721 aacagaaact gatctcggag gaagatctga acttaattaa aacctgcagg atgcctgttc
     3781 tggaaaaccg ggctgctcag ggcgatatta ctgcacccgg cggtgctcgc cgtttaacgg
     3841 gtgatcagac tgccgctctg cgtgattctc ttagcgataa acctgcaaaa aatattattt
     3901 tgctgattgg cgatgggatg ggggactcgg aaattactgc cgcacgtaat tatgccgaag
     3961 gtgcgggcgg cttttttaaa ggtatagatg ccttaccgct taccgggcaa tacactcact
     4021 atgcgctgaa taaaaaaacc ggcaaaccgg actacgtcac cgactcggct gcatcagcaa
     4081 ccgcctggtc aaccggtgtc aaaacctata acggcgcgct gggcgtcgat attcacgaaa
     4141 aagatcaccc aacgattctg gaaatggcaa aagccgcagg tctggcgacc ggtaacgttt
     4201 ctaccgcaga gttgcaggat gccacgcccg ctgcgctggt ggcacatgtg acctcgcgca
     4261 aatgctacgg tccgagcgcg accagtgaaa aatgtccggg taacgctctg gaaaaaggcg
     4321 gaaaaggatc gattaccgaa cagctgctta acgctcgtgc cgacgttacg cttggcggcg
     4381 gcgcaaaaac ctttgctgaa acggcaaccg ctggtgaatg gcagggaaaa acgctgcgtg
     4441 aacaggcaca ggcgcgtggt tatcagttgg tgagcgatgc tgcctcactg aattcggtga
     4501 cggaagcgaa tcagcaaaaa cccctgcttg gcctgtttgc tgacggcaat atgccagtgc
     4561 gctggctagg accgaaagca acgtaccatg gcaatatcga taagcccgca gtcacctgta
     4621 cgccaaatcc gcaacgtaat gacagtgtac caaccctggc gcagatgacc gacaaagcca
     4681 ttgaattgtt gagtaaaaat gagaaaggct ttttcctgca agttgaaggt gcgtcaatcg
     4741 ataaacagga tcatgctgcg aatccttgtg ggcaaattgg cgagacggtc gatctcgatg
     4801 aagccgtaca acgggcgctg gaattcgcta aaaaggaggg taacacgctg gtcatagtca
     4861 ccgctgatca cgcccacgcc agccagattg ttgcgccgga taccaaagct ccgggcctca
     4921 cccaggcgct aaataccaaa gatggcgcag tgatggtgat gagttacggg aactccgaag
     4981 aggattcaca agaacatacc ggcagtcagt tgcgtattgc ggcgtatggc ccgcatgccg
     5041 ccaatgttgt tggactgacc gaccagaccg atctcttcta caccatgaaa gccgctctgg
     5101 ggctgaaata aaaccgcgcc cggcagtgaa ttttcgctgc cgggtggttt ttttgctgtt
     5161 agcaaccaga cttaatggca gatcacgggc gcatacgctc atggttaaaa catgaagagg
     5221 gatggtgcta tgaaaataac attactggtt accttgcttt tcggtctggt ttttttaacc
     5281 accgtcggcg ctgccgagag aactttaacc ccacaacaac agcgtatgac ctcctgtaat
     5341 cagcaggcga cggcgcaggc gttgaaaggg gatgctcgta agacctacat gagtgattgc
     5401 ctgaagaaca gcaagtctgc gcctggcgaa aaaagtttga cgccacagca gcaaaagatg
     5461 cgcgaatgca ataatcaagc aacacaacaa tctctgaaag gtgatgatcg taataagttt
     5521 atgagtgcct gcctcaagaa agccgcctga tacctgatag tgctaacggg tgagctacga
     5581 aaatggctca cccgaaatat catacttctg cctttagctc cgtctctata atttgggaaa
     5641 attgtttctg aatgttccca aaaataatga atgatgaaaa ctttttcaaa aaagcggcgg
     5701 cgcacgggga ggaacctcct ttaactcctc aaaacgaaca tcagcggtcc gggctgcgct
     5761 tcgcccgtcg cgtcagacta ccccgtgcgg ttggcctggc tggcatgttc ttaccgattg
     5821 cttcaacgct ggtttcacac ccgccgccgg gctggtggtg gctggtgttg gtcggctggg
     5881 cgttcgtctg gccgcattta gcctggcaga tagcgagcag ggccgtcgat ccgcttagcc
     5941 gggaaattta caacttaaaa accgatgcag tattagcggg aatgtgggta ggcgtaatgg
     6001 gcgtaaacgt gctgccttcc accgcgatgt tgatgattat gtgtctgaat ttgatggggg
     6061 caggcggccc ccgtctgttt gtcgcgggtc tggtgttgat ggtggtttcc tgccttgtca
     6121 ccggccggcc ggatccgaat tcgcgttaat attttgttaa aattcgcgtt aaatttttgt
     6181 taaatcagct cattttttaa ccaataggcc gactgcgatg agtggcaggg cggggcgtaa
     6241 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
     6301 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
     6361 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
     6421 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga
     6481 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggtgaa
     6541 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
     6601 cgacgcaacg cggggaggca gacaaggtat agggcggcga ggcggctaca gccgatagtc
     6661 tggaacagcg cacttacggg ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac
     6721 ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg
     6781 caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg
     6841 ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg
     6901 ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc
     6961 gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc
     7021 gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc
     7081 gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac
     7141 cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt
     7201 ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc
     7261 aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt
     7321 gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta
     7381 gggtgccgca cggttgcggc accatgcgca atcagctgca acttttcggc agcgcgacaa
     7441 caattatgcg ttgcgtaaaa gtggcagtca attacagatt ttctttaacc tacgcaatga
     7501 gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc ccttttttaa gttgttgatt
     7561 tttaagtctt tcgcatttcg ccctatatct agttctttgg tgcccaaaga agggcacccc
     7621 tgcggggttc ccccacgcct tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg
     7681 ggcttgttga tcgactgcgc ggccttcggc cttgcccaag gtggcgctgc ccccttggaa
     7741 cccccgcact cgccgccgtg aggctcgggg ggcaggcggg cgggcttcgc cttcgactgc
     7801 ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg
     7861 ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc
     7921 cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg
     7981 ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct
     8041 gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag ggttgtccac
     8101 gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca tatccacggg
     8161 ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc cgtagggcgc
     8221 cgcagccgcc gtaggcggtc acgactttgc gaagcaaagt ctagtgagta tactcaagca
     8281 ttgagtggcc cgccggaggc accgccttgc gctgcccccg tcgagccggt tggacaccaa
     8341 aagggagggg caggcatggc ggcatacgcg atcatgcgat gcaagaagct ggcgaaaatg
     8401 ggcaacgtgg cggccagtct caagcacgcc taccgcgagc gcgagacgcc caacgctgac
     8461 gccagcagga cgccagagaa cgagcactgg gcggccagca gcaccgatga agcgatgggc
     8521 cgactgcgcg agttgctgcc agagaagcgg cgcaaggacg ctgtgttggc ggtcgagtac
     8581 gtcatgacgg ccagcccgga atggtggaag tcggccagcc aagaacagca ggcggcgttc
     8641 ttcgagaagg cgcacaagtg gctggcggac aagtacgggg cggatcgcat cgtgacggcc
     8701 agcatccacc gtgacgaaac cagcccgcac atgaccgcgt tcgtggtgcc gctgacgcag
     8761 gacggcaggc tgtcggccaa ggagttcatc ggcaacaaag cgcagatgac ccgcgaccag
     8821 accacgtttg cggccgctgt ggccgatcta gggctgcaac ggggcatcga gggcagcaag
     8881 gcacgtcaca cgcgcattca ggcgttctac gaggccctgg agcggccacc agtgggccac
     8941 gtcaccatca gcccgcaagc ggtcgagcca cgcgcctatg caccgcaggg attggccgaa
     9001 aagctgggaa tctcaaagcg cgttgagacg ccggaagccg tggccgaccg gctgacaaaa
     9061 gcggttcggc aggggtatga gcctgcccta caggccgccg caggagcgcg tgagatgcgc
     9121 aagaaggccg atcaagccca agagacggcc cgag
//
