Basic Vector Information
- Vector Name:
- pNB20
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5709 bp
- Type:
- Cloning vector
- Replication origin:
- pBBR1 oriV
- Source/Author:
- Bongio NJ, Lampe DJ.
- Promoter:
- lac
pNB20 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNB20 vector Sequence
LOCUS 40924_33018 5709 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNB20, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5709)
AUTHORS Bongio NJ, Lampe DJ.
TITLE Inhibition of Plasmodium berghei Development in Mosquitoes by
Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel
Native Secretion Signal
JOURNAL PLoS ONE 10 (12), E0143541 (2015)
PUBMED 26636338
REFERENCE 2 (bases 1 to 5709)
AUTHORS Bongio NJ.
TITLE Direct Submission
JOURNAL Submitted (13-SEP-2015) Biology, Shenandoah University, 1460
University Drive, Winchester, VA 22601, USA
REFERENCE 3 (bases 1 to 5709)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5709)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2015"; volume: "10"; issue: "12"; pages: "E0143541"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-SEP-2015) Biology, Shenandoah University, 1460 University Drive,
Winchester, VA 22601, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5709
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 458..1249
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
protein_bind 1657..1678
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1693..1723
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1731..1747
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
regulatory 1767..1783
/label=translational enhancer
/note="translational enhancer"
/regulatory_class="enhancer"
CDS 1791..2648
/label=AmpR
/note="beta-lactamase"
CDS complement(3258..3917)
/label=pBBR1 Rep
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
rep_origin complement(3918..4687)
/direction=LEFT
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
This page is informational only.