LOCUS       40924_33018        5709 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNB20, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Bongio NJ, Lampe DJ.
  TITLE     Inhibition of Plasmodium berghei Development in Mosquitoes by 
            Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel 
            Native Secretion Signal
  JOURNAL   PLoS ONE 10 (12), E0143541 (2015)
  PUBMED    26636338
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Bongio NJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2015) Biology, Shenandoah University, 1460 
            University Drive, Winchester, VA 22601, USA
REFERENCE   3  (bases 1 to 5709)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "12"; pages: "E0143541"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-SEP-2015) Biology, Shenandoah University, 1460 University Drive,
            Winchester, VA 22601, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    1657..1678
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1693..1723
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1731..1747
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      1767..1783
                     /label=translational enhancer
                     /note="translational enhancer"
                     /regulatory_class="enhancer"
     CDS             1791..2648
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(3258..3917)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(3918..4687)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca tgcgcccaat
     1561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1741 ataacaattt cacacaggaa acagctttaa ctttaagaag gagactgcat atgagtattc
     1801 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1861 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1921 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1981 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2041 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2101 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2161 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2221 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2281 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2341 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2401 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2461 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2521 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2581 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2641 agcattggta actgtcagac cttaattaac ctgcaggggc cggccggatc cgaattcgcg
     2701 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     2761 aggccgactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc
     2821 cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     2881 gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc
     2941 tattgctggt ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat
     3001 gcctgaggcc agtttgctca ggctctcccc gtggaggtaa taattgacga tatgatcatt
     3061 tattctgcct cccagagcct gataaaaacg gtgaatccgt tagcgaggtg ccgccggctt
     3121 ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa
     3181 ggtatagggc ggcgaggcgg ctacagccga tagtctggaa cagcgcactt acgggttgct
     3241 gcgcaaccca agtgctaccg gcgcggcagc gtgacccgtg tcggcggctc caacggctcg
     3301 ccatcgtcca gaaaacacgg ctcatcgggc atcggcaggc gctgctgccc gcgccgttcc
     3361 cattcctccg tttcggtcaa ggctggcagg tctggttcca tgcccggaat gccgggctgg
     3421 ctgggcggct cctcgccggg gccggtcggt agttgctgct cgcccggata cagggtcggg
     3481 atgcggcgca ggtcgccatg ccccaacagc gattcgtcct ggtcgtcgtg atcaaccacc
     3541 acggcggcac tgaacaccga caggcgcaac tggtcgcggg gctggcccca cgccacgcgg
     3601 tcattgacca cgtaggccga cacggtgccg gggccgttga gcttcacgac ggagatccag
     3661 cgctcggcca ccaagtcctt gactgcgtat tggaccgtcc gcaaagaacg tccgatgagc
     3721 ttggaaagtg tcttctggct gaccaccacg gcgttctggt ggcccatctg cgccacgagg
     3781 tgatgcagca gcattgccgc cgtgggtttc ctcgcaataa gcccggccca cgcctcatgc
     3841 gctttgcgtt ccgtttgcac ccagtgaccg ggcttgttct tggcttgaat gccgatttct
     3901 ctggactgcg tggccatgct tatctccatg cggtagggtg ccgcacggtt gcggcaccat
     3961 gcgcaatcag ctgcaacttt tcggcagcgc gacaacaatt atgcgttgcg taaaagtggc
     4021 agtcaattac agattttctt taacctacgc aatgagctat tgcggggggt gccgcaatga
     4081 gctgttgcgt accccccttt tttaagttgt tgatttttaa gtctttcgca tttcgcccta
     4141 tatctagttc tttggtgccc aaagaagggc acccctgcgg ggttccccca cgccttcggc
     4201 gcggctcccc ctccggcaaa aagtggcccc tccggggctt gttgatcgac tgcgcggcct
     4261 tcggccttgc ccaaggtggc gctgccccct tggaaccccc gcactcgccg ccgtgaggct
     4321 cggggggcag gcgggcgggc ttcgccttcg actgccccca ctcgcatagg cttgggtcgt
     4381 tccaggcgcg tcaaggccaa gccgctgcgc ggtcgctgcg cgagccttga cccgccttcc
     4441 acttggtgtc caaccggcaa gcgaagcgcg caggccgcag gccggaggct tttccccaga
     4501 gaaaattaaa aaaattgatg gggcaaggcc gcaggccgcg cagttggagc cggtgggtat
     4561 gtggtcgaag gctgggtagc cggtgggcaa tccctgtggt caagctcgtg ggcaggcgca
     4621 gcctgtccat cagcttgtcc agcagggttg tccacgggcc gagcgaagcg agccagccgg
     4681 tggccgctcg cggccatcgt ccacatatcc acgggctggc aagggagcgc agcgaccgcg
     4741 cagggcgaag cccggagagc aagcccgtag ggcgccgcag ccgccgtagg cggtcacgac
     4801 tttgcgaagc aaagtctagt gagtatactc aagcattgag tggcccgccg gaggcaccgc
     4861 cttgcgctgc ccccgtcgag ccggttggac accaaaaggg aggggcaggc atggcggcat
     4921 acgcgatcat gcgatgcaag aagctggcga aaatgggcaa cgtggcggcc agtctcaagc
     4981 acgcctaccg cgagcgcgag acgcccaacg ctgacgccag caggacgcca gagaacgagc
     5041 actgggcggc cagcagcacc gatgaagcga tgggccgact gcgcgagttg ctgccagaga
     5101 agcggcgcaa ggacgctgtg ttggcggtcg agtacgtcat gacggccagc ccggaatggt
     5161 ggaagtcggc cagccaagaa cagcaggcgg cgttcttcga gaaggcgcac aagtggctgg
     5221 cggacaagta cggggcggat cgcatcgtga cggccagcat ccaccgtgac gaaaccagcc
     5281 cgcacatgac cgcgttcgtg gtgccgctga cgcaggacgg caggctgtcg gccaaggagt
     5341 tcatcggcaa caaagcgcag atgacccgcg accagaccac gtttgcggcc gctgtggccg
     5401 atctagggct gcaacggggc atcgagggca gcaaggcacg tcacacgcgc attcaggcgt
     5461 tctacgaggc cctggagcgg ccaccagtgg gccacgtcac catcagcccg caagcggtcg
     5521 agccacgcgc ctatgcaccg cagggattgg ccgaaaagct gggaatctca aagcgcgttg
     5581 agacgccgga agccgtggcc gaccggctga caaaagcggt tcggcagggg tatgagcctg
     5641 ccctacaggc cgccgcagga gcgcgtgaga tgcgcaagaa ggccgatcaa gcccaagaga
     5701 cggcccgag
//
