Basic Vector Information
- Vector Name:
- pNAK1
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 5223 bp
- Type:
- Shuttle vector
- Replication origin:
- ori
- Source/Author:
- Nakayama S, Irie R, Kosaka T, Matsuura K, Yoshino S, Furukawa K.
pNAK1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNAK1 vector Sequence
LOCUS 40924_32983 5223 bp DNA circular SYN 18-DEC-2018
DEFINITION Shuttle vector pNAK1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5223)
AUTHORS Nakayama S, Irie R, Kosaka T, Matsuura K, Yoshino S, Furukawa K.
TITLE New host-vector system in solvent-producing Clostridium
saccharoperbutylacetonicum strain N1-4
JOURNAL J. Gen. Appl. Microbiol. 53 (1), 53-56 (2007)
PUBMED 17429161
REFERENCE 2 (bases 1 to 5223)
AUTHORS Nakayama S, Kousaka T, Matsuura K, Yoshino S, Furukawa K.
TITLE Direct Submission
JOURNAL Submitted (16-NOV-2004) Laboratory of Applied Microbiology, Kyushu
University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan
REFERENCE 3 (bases 1 to 5223)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5223)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Gen.
Appl. Microbiol."; date: "2007"; volume: "53"; issue: "1"; pages:
"53-56"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(16-NOV-2004) Laboratory of Applied Microbiology, Kyushu University,
Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5223
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 6..24
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature 37..145
/label=MCS
/note="pBluescript multiple cloning site"
promoter complement(154..172)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 795..1265
/codon_start=1
/gene="rep"
/product="replication protein"
/label=rep
/protein_id="AAV86077.1"
/translation="MLLTNKGVIGLSKEDRVLYAGSDEWVNTRTGETITANQIIKKTDR
NGFMITYLSAIINLIETLGNRKMQVVKYILENMDKSNNTLIITNRELAEKSKASLNTVV
KTIAILKKAKIITTRTGAIMITPELVHRGSKSKEQYLLTKFQEFEEVKSNEI"
gene 795..1265
/gene="rep"
/label=rep
CDS complement(1323..2144)
/codon_start=1
/gene="mob"
/product="mobilization protein"
/label=mob
/protein_id="AAV86078.1"
/translation="MSIFKIRSVRKMWYQQWFTWTRPKGXGVAPHMHFSFVPMNEDGTL
SAKKKINRNFLREIQDEFPRYMKGYGFNVERGMENSTKKHLEPLEFKKQELKKDIENAK
QEQERFEKKYSNFKESIKTRAENLQKTSESLSKLEEHTKGVLGKLDKIQVKKGLFGDKL
TISEQDYSILVSLAKAGEGKLIENLQLRSKIGDXTQKLSKLDAINNEFQRLKGIELKHF
ELQRKYRNLNISFERVEKAINNLDLVEPVNKEIQSVKIAEMSISKSFDIER"
gene complement(1323..2144)
/gene="mob"
/label=mob
CDS 3190..3846
/codon_start=1
/label=CmR
/note="chloramphenicol acetyltransferase"
/translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 4284..4370
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin 4433..5021
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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