LOCUS       40924_32983        5223 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pNAK1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Nakayama S, Irie R, Kosaka T, Matsuura K, Yoshino S, Furukawa K.
  TITLE     New host-vector system in solvent-producing Clostridium 
            saccharoperbutylacetonicum strain N1-4
  JOURNAL   J. Gen. Appl. Microbiol. 53 (1), 53-56 (2007)
  PUBMED    17429161
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Nakayama S, Kousaka T, Matsuura K, Yoshino S, Furukawa K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2004) Laboratory of Applied Microbiology, Kyushu 
            University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan
REFERENCE   3  (bases 1 to 5223)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5223)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Gen. 
            Appl. Microbiol."; date: "2007"; volume: "53"; issue: "1"; pages: 
            "53-56"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-NOV-2004) Laboratory of Applied Microbiology, Kyushu University,
            Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..24
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    37..145
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(154..172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             795..1265
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /protein_id="AAV86077.1"
                     /translation="MLLTNKGVIGLSKEDRVLYAGSDEWVNTRTGETITANQIIKKTDR
                     NGFMITYLSAIINLIETLGNRKMQVVKYILENMDKSNNTLIITNRELAEKSKASLNTVV
                     KTIAILKKAKIITTRTGAIMITPELVHRGSKSKEQYLLTKFQEFEEVKSNEI"
     gene            795..1265
                     /gene="rep"
                     /label=rep
     CDS             complement(1323..2144)
                     /codon_start=1
                     /gene="mob"
                     /product="mobilization protein"
                     /label=mob
                     /protein_id="AAV86078.1"
                     /translation="MSIFKIRSVRKMWYQQWFTWTRPKGXGVAPHMHFSFVPMNEDGTL
                     SAKKKINRNFLREIQDEFPRYMKGYGFNVERGMENSTKKHLEPLEFKKQELKKDIENAK
                     QEQERFEKKYSNFKESIKTRAENLQKTSESLSKLEEHTKGVLGKLDKIQVKKGLFGDKL
                     TISEQDYSILVSLAKAGEGKLIENLQLRSKIGDXTQKLSKLDAINNEFQRLKGIELKHF
                     ELQRKYRNLNISFERVEKAINNLDLVEPVNKEIQSVKIAEMSISKSFDIER"
     gene            complement(1323..2144)
                     /gene="mob"
                     /label=mob
     CDS             3190..3846
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      4284..4370
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      4433..5021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcgcaatta accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc
       61 tctagaacta gtggatcccc ccgggctgca ggaattcgat atcaagctta tcgataccgt
      121 cgacctcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgcc
      181 ggcgatgtaa ttattggtgc atataccgag acagatgccg atggttatga atgggataat
      241 gtagtaggtg gagatgctgg agtgaagcca ggatggatta gagatgatct tctaggttat
      301 ccccaccatt aataatttta tcaagtaaaa aagagccatt ggctcttttt tattttttta
      361 tttgttgtat acctgtcgaa cccaataacc caaagacttc ttcctctatt aacttactac
      421 gtagccccct tgtggggcat ttaagaagcc catagaattg ttttccatgt tgagtattat
      481 ttgtttgtca cgtgcccatc cggcgtaagg atagaaggcg aacaactttt tatggattta
      541 tgaaaatagc tatttgagcc gtgcgacagc ttactcttaa tataagaaaa gagtaaattt
      601 atagttaaca ttctattaac ccttattata tctagtttta gagtattttt gtttgtattg
      661 atttttcaat acatttgtat cgatttttcg atacacttta atttgtaatt tttgcatttt
      721 tttgtaaaat aatatttttt tgctaccttt tttttgcaac atttgtattg ttttttcaat
      781 acgcacatga tattatgtta ttaacaaata agggggttat tggattgagt aaggaagata
      841 gggtgttgta tgccggttca gatgaatggg ttaatacaag aactggagaa actataactg
      901 caaatcaaat aattaaaaag actgatagaa atggatttat gataacttat ctatcagcaa
      961 ttattaattt gattgaaaca ttaggtaatc gtaaaatgca agttgttaaa tatattctag
     1021 aaaacatgga caagtccaat aatacattaa taataaccaa tagagaattg gctgaaaaat
     1081 caaaggcttc cttaaataca gttgtaaaaa caatagccat acttaaaaag gctaaaatca
     1141 taacaactag aactggggct ataatgataa ctccagaatt agtacataga ggtagcaagt
     1201 ccaaagagca atacttatta actaaatttc aagagtttga agaagtaaag tcaaatgaaa
     1261 tatgataatg gcagcataaa avataaaggc ggttcagcaa cagatctgaa tcgcctttat
     1321 ttttatcgtt ctatatcaaa agacttagaa atagacattt cagctatctt tacagattgt
     1381 atctccttgt ttactggttc tactagatct aaattattta ttgccttttc tacacgttca
     1441 aaagaaatat taaggtttct gtattttcgt tggagttcaa aatgtttaag ttctatccct
     1501 ttaagcctct gaaattcatt atttatggca tcaagcttac ttaatttctg agtkaagtct
     1561 ccaattttac tccttaattg tagattttca attaattttc cttccccagc tttagctaga
     1621 ctgactaaaa tactatagtc ttgttcagat atggttaatt tatcgccaaa taaccccttc
     1681 ttaacttgta tcttatctaa ttttcctaat accccttttg tatgttcttc aagcttagaa
     1741 agactctctg atgtcttctg aaggttttct gctctagtct taatactttc cttaaaattt
     1801 gagtatttct tttcaaatct ttcctgctcc tgcttggcat tttcaatatc tttctttagc
     1861 tcctgtttct tgaattctaa aggttctaag tgcttttttg tggaattctc cattcctctc
     1921 tcaacgttaa agccatatcc tttcatatag cgaggaaatt catcttgaat ctctctaaga
     1981 aaattacggt taattttctt ctttgctgaa agagttccat cttcattcat tggtacaaag
     2041 ctgaaatgca tatggggggc taccccaawt ccctttggtc tcgtccatgt gaaccactgc
     2101 tgataccaca tttttctcac cgaccttatt ttgaagatac tcatagacct tctcaaaaaa
     2161 atctcctggt atcttcaggg gaaagtttct tgaaaaactt cttatccgaa cccactacag
     2221 tatcaacata gactattgca tctttacgta ctgctttatc agaggttctg ttctctttaa
     2281 taatctgatc tattttgtta aggtatttta tactctcttg attttgaaga tcatagttta
     2341 aatggctctt tgaaagatcg atattttcat tgcccataat tcttattttc acgttgatta
     2401 tgcttttgta atcctactac atttcctcta gtatatttgc ccatatgaca tactaaatga
     2461 ctcaaaatta ccacctcacc cgaatacttt agatatgaaa tttcatatct aaagtataac
     2521 tcactatact ttacatgtaa agttgttcgc ccctaacggg attaaagggg gtttccccct
     2581 cgctacgctc accccatgat cttttaaaaa agatcaacca aaattttaat tgtacctacc
     2641 gtacaggtag gaagttgcaa ccttagggtt gcaacttcgt tgctctagta tgtagtggtt
     2701 attgctccat caagggcaag acgagtcagg caagctgacc cttgattacg cctaaagtta
     2761 aatatgtata ttgggtcaca tgtaaccatt ttatgtcata agaggttgaa atatggacta
     2821 aatggttgta taatgaattg tatacataat ttgggaggta taataaaatg aaaaaaataa
     2881 aaaagttatt aagtttagga cttttaactg cttgtgtagt gacaacttta gcagttccag
     2941 cctttgcaaa atcatctgtt gagatgacat tgccaaaaaa tcctacagca ttgccaaaat
     3001 ctacagttgc tgaatcaaag gcagagtttg gtgtacatag agttgttgca gttgatgctg
     3061 caagaataag aaaaggtcct ggaacaaaat atggtatagc atgcacagta acccgagccg
     3121 gatcgccaag cttgagtagg acaaatccgc cgagcttcga cgagattttc aggagctaag
     3181 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatcgcat
     3241 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     3301 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     3361 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     3421 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     3481 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     3541 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     3601 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     3661 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     3721 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat
     3781 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     3841 ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa
     3901 taagtgataa taagcggatg aatggcagaa attcgtcgag gcggcacctc gctaacggat
     3961 tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca
     4021 acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct
     4081 cggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
     4141 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
     4201 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
     4261 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     4321 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggtt
     4381 cgacggagtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4441 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4501 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4561 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4621 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4681 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4741 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4801 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
     4861 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     4921 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     4981 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5041 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5101 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5161 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agaagctcat tcgccatgca
     5221 tcg
//
