Basic Vector Information
- Vector Name:
- pN-TAPa
- Antibiotic Resistance:
- Streptomycin
- Length:
- 12537 bp
- Type:
- N-terminal TAPa T-DNA vector
- Replication origin:
- ori
- Source/Author:
- Rubio V, Shen Y, Saijo Y, Liu Y, Gusmaroli G, Dinesh-Kumar SP, Deng XW.
- Promoter:
- CaMV35S(enhanced)
pN-TAPa vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pN-TAPa vector Sequence
LOCUS 40924_32968 12537 bp DNA circular SYN 18-DEC-2018
DEFINITION N-terminal TAPa T-DNA vector pN-TAPa, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12537)
AUTHORS Rubio V, Shen Y, Saijo Y, Liu Y, Gusmaroli G, Dinesh-Kumar SP, Deng
XW.
TITLE An alternative tandem affinity purification strategy applied to
Arabidopsis protein complex isolation
JOURNAL Plant J. 41 (5), 767-778 (2005)
PUBMED 15703063
REFERENCE 2 (bases 1 to 12537)
AUTHORS Rubio V, Deng XW.
TITLE Direct Submission
JOURNAL Submitted (21-OCT-2004) MCDB, Yale University, 165, Prospect St.,
New Haven, CT 06511, USA
REFERENCE 3 (bases 1 to 12537)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12537)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
date: "2005"; volume: "41"; issue: "5"; pages: "767-778"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(21-OCT-2004) MCDB, Yale University, 165, Prospect St., New Haven,
CT 06511, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12537
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 96..769
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
misc_feature 791..846
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
regulatory 857..866
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 884..1057
/label=ProtA
/note="IgG-binding unit of Staphylococcus aureus protein A"
CDS 1061..1231
/codon_start=1
/product="IgG-binding unit of Staphylococcus aureus protein
A"
/label=ProtA
/translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
EAKKLNDAQAPK"
CDS 1256..1279
/label=HRV 3C site
/note="recognition and cleavage site for human rhinovirus
3C and PreScission proteases"
CDS 1286..1303
/label=6xHis
/note="6xHis affinity tag"
CDS 1328..1357
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
CDS 1364..1393
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1400..1429
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1448..1477
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1484..1513
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1520..1549
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1568..1597
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1604..1633
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1640..1669
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
protein_bind 1704..1828
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 2002..2104
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 2105..2761
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 3106..3408
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(3452..3576)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
terminator 3605..3857
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(3873..3889)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3897..3913
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3921..3951)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3966..3987)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 4152..4176
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 5476..6102
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 6539..7603
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 7672..7866
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 8210..8350
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(8536..9124)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9371..10159)
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
misc_feature 10687..10711
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal complement(10809..10983)
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(11021..11551)
/label=GmR
/note="gentamycin acetyltransferase"
promoter complement(11611..12289)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind 12518..12534
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
This page is informational only.