LOCUS       40924_32968       12537 bp DNA     circular SYN 18-DEC-2018
DEFINITION  N-terminal TAPa T-DNA vector pN-TAPa, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12537)
  AUTHORS   Rubio V, Shen Y, Saijo Y, Liu Y, Gusmaroli G, Dinesh-Kumar SP, Deng 
            XW.
  TITLE     An alternative tandem affinity purification strategy applied to 
            Arabidopsis protein complex isolation
  JOURNAL   Plant J. 41 (5), 767-778 (2005)
  PUBMED    15703063
REFERENCE   2  (bases 1 to 12537)
  AUTHORS   Rubio V, Deng XW.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2004) MCDB, Yale University, 165, Prospect St., 
            New Haven, CT 06511, USA
REFERENCE   3  (bases 1 to 12537)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12537)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2005"; volume: "41"; issue: "5"; pages: "767-778"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-OCT-2004) MCDB, Yale University, 165, Prospect St., New Haven, 
            CT 06511, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..769
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    791..846
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     regulatory      857..866
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             884..1057
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     CDS             1061..1231
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNDAQAPK"
     CDS             1256..1279
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             1286..1303
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1328..1357
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1364..1393
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1400..1429
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1448..1477
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1484..1513
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1520..1549
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1568..1597
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1604..1633
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1640..1669
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1704..1828
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2002..2104
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2105..2761
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3106..3408
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3452..3576)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3605..3857
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3873..3889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3897..3913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3921..3951)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3966..3987)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    4152..4176
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5476..6102
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6539..7603
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7672..7866
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8210..8350
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8536..9124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9371..10159)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    10687..10711
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10809..10983)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11021..11551)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(11611..12289)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     primer_bind     12518..12534
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagaggc cggtctagag
      781 tatttttaca acaattacca acaacaacaa acaacaaaca acattacaat tactatttac
      841 aattacaaga tcgatggcca ccatggcgca acacgaagaa gccgtagaca acaaattcaa
      901 taaagaacaa caaaacgcgt tctatgagat cttacattta cctaacttaa acgaagaaca
      961 acgaaacgcc ttcatccaaa gtttaaaaga tgacccaagc caaagcgcta accttttagc
     1021 agaagctaaa aagctaaatg atgctcaggc gccgaaagta gacaacaaat tcaacaaaga
     1081 acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa
     1141 cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc
     1201 taaaaagcta aatgatgctc aggcgccgaa agtagacgcg aatttgagct cggtactgga
     1261 agttctgttc caggggcccg aattccatca ccatcaccat cacgcgggcc gctctagaac
     1321 tagtggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc
     1381 cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa gaagacttga acggatcctc
     1441 tagaggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc
     1501 cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa gaagacttga acggatcctc
     1561 tagaggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc
     1621 cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa gaagacttga acggatccac
     1681 tagtggatcc cccgggctcg agaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     1741 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     1801 gtaaaacaca acatatccag tcactatggc ggccgcgggt gatgctgcca acttagcggc
     1861 cgctaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg tgacggaaga
     1921 tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa
     1981 cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga
     2041 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
     2101 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     2161 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     2221 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     2281 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     2341 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     2401 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     2461 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     2521 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     2581 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     2641 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt
     2701 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     2761 gtaatctaga ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     2821 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     2881 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     2941 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     3001 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     3061 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     3121 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     3181 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     3241 gtcccccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     3301 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     3361 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     3421 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca
     3481 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     3541 ttacgtttct cgttcagctt tcttgtacaa agtggtttaa ggtaccgccc gggcgagctc
     3601 ggaagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     3661 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     3721 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     3781 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     3841 atctatgtta ctagatcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3901 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3961 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4021 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4081 cgtattggct agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa
     4141 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     4201 aataacggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     4261 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
     4321 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt
     4381 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
     4441 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
     4501 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
     4561 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
     4621 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
     4681 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
     4741 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
     4801 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
     4861 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
     4921 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
     4981 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
     5041 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
     5101 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
     5161 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
     5221 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc
     5281 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     5341 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     5401 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
     5461 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     5521 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     5581 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     5641 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     5701 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     5761 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     5821 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     5881 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     5941 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     6001 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     6061 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     6121 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     6181 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     6241 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     6301 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     6361 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     6421 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     6481 gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgacgg
     6541 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     6601 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     6661 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     6721 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     6781 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     6841 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     6901 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     6961 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     7021 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     7081 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     7141 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     7201 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     7261 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
     7321 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
     7381 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
     7441 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
     7501 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
     7561 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
     7621 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag
     7681 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc
     7741 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg
     7801 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg
     7861 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc
     7921 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa
     7981 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact
     8041 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa
     8101 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     8161 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     8221 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     8281 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     8341 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8941 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9181 ttaagggatt ttggtcatgc atgatatatc tcccaatttg tgtagggctt attatgcacg
     9241 cttaaaaata ataaaagcag acttgacctg atagtttggc tgtgagcaat tatgtgctta
     9301 gtgcatctaa tcgcttgagt taacgccggc gaagcggcgt cggcttgaac gaatttctag
     9361 ctagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
     9421 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
     9481 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
     9541 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
     9601 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
     9661 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
     9721 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
     9781 tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca
     9841 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
     9901 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
     9961 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
    10021 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
    10081 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
    10141 cgatcaccgc ttcccccatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa
    10201 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
    10261 atgcccgagg catagactgt accccaaaaa aacatgtcat aacaagaagc catgaaaacc
    10321 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgacggc
    10381 agttacgcta cttgcattac agcttacgaa ccgaacgagg cttatgtcca ctgggttcgt
    10441 gcccgaattg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
    10501 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
    10561 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
    10621 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
    10681 ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac
    10741 attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga tctataggga
    10801 ctttaggtga tctggatttt agtactggat tttggtttta ggaattagaa attttattga
    10861 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacgtgag
    10921 cgaaacccta taagaaccct aattccctta tctgggaact actcacacat tattatggag
    10981 aaactcgatg tcgatcgact ctagctagaa cgaattgtta ggtggcggta cttgggtcga
    11041 tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat
    11101 cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct
    11161 tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga
    11221 gatcatagat atagatctca ctacgcggct gctcaaacct gggcagaacg taagccgcga
    11281 gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga
    11341 gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga
    11401 actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc
    11461 tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct
    11521 gctgcgtaac atcgttgctg ctgcgtacca tggtcgatcg acagatctgc gaatcgagag
    11581 agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact
    11641 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
    11701 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
    11761 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
    11821 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
    11881 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
    11941 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
    12001 cttggagtag acgagagtgt cgtgctccac catgttatca catcaatcca cttgctttga
    12061 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
    12121 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
    12181 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
    12241 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
    12301 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
    12361 gttggcaagc tgctctagca ttcgccattc aggctgcgca actgttggga agggcgatcg
    12421 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    12481 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgcc
//
