Basic Vector Information
- Vector Name:
- pMycoFos
- Antibiotic Resistance:
- Kanamycin
- Length:
- 12530 bp
- Type:
- Shuttle vector
- Replication origin:
- ori2
- Source/Author:
- Ly MA, Liew EF, Le NB, Coleman NV.
pMycoFos vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMycoFos vector Sequence
LOCUS 40924_32923 12530 bp DNA circular SYN 18-DEC-2018
DEFINITION Shuttle vector pMycoFos, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12530)
AUTHORS Ly MA, Liew EF, Le NB, Coleman NV.
TITLE Construction and evaluation of pMycoFos, a fosmid shuttle vector for
Mycobacterium spp. with inducible gene expression and copy number
control
JOURNAL J. Microbiol. Methods 86 (3), 320-326 (2011)
PUBMED 21689690
REFERENCE 2 (bases 1 to 12530)
AUTHORS Ly MA, Coleman NV.
TITLE Direct Submission
JOURNAL Submitted (08-OCT-2010) School of Molecular Bioscience, University
of Sydney, Maze Crescent, Darlington, NSW 2006, Australia
REFERENCE 3 (bases 1 to 12530)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12530)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J.
Microbiol. Methods"; date: "2011"; volume: "86"; issue: "3"; pages:
"320-326"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(08-OCT-2010) School of Molecular Bioscience, University of Sydney,
Maze Crescent, Darlington, NSW 2006, Australia"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12530
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 83..1979
/note="oriM; derived from pAL5000"
CDS complement(2030..3103)
/codon_start=1
/gene="amiC"
/product="AmiC"
/label=amiC
/note="acetamidase regulator"
/protein_id="ADR02799.1"
/translation="MQDGEVEFRVGLVIPLQGPAGIFAPSCEAVAELAAKEVNDRGGLQ
GRKVTIEVLDGGRPGDDVARTVADRLRGHGLDAVTGWHISAVRNRISPVVRDRIPYVYT
SLYEGGERTPGVFCTGETPQIQIAPALAWLRDHFGIRSWCLVGDDYIWPRRSAAAARAY
CRDLDLELRREIYVPYGTDDFRAPVRKAIASGAQAVLMLLVGQDAVLFNREFARAGGHD
RMARFSPLMEENMLLASGAGSTENLYVAAAYFSSLATAGAMDLMGSYVARYGADAPPLN
AMAESCYEGLVALEAIFQRAHSPEIPDLMASAHDVGFDGPRGPMCMRDSQFDQQVYIAS
ADGYDFDILDTLTTLDA"
gene complement(2030..3103)
/gene="amiC"
/label=amiC
CDS 3206..3634
/codon_start=1
/gene="amiA"
/product="AmiA"
/label=amiA
/note="acetamidase regulator"
/protein_id="ADR02800.1"
/translation="MAAGQQRRPNLLLPLVRLTHLAESAIERVLADSSLKIEDWRVLDE
LAGRRTVPMSDLAQATLITGPTLTRTVDRLVSQGIIYRTADLHDRRRVLVALTPRGRTL
RNRLVDAVAEAECAAFESCGLDVDQLRELVDTTSNLTS"
gene 3206..3634
/gene="amiA"
/label=amiA
CDS 3714..3998
/codon_start=1
/gene="amiD"
/product="AmiD"
/label=amiD
/note="acetamidase regulator"
/protein_id="ADR02801.1"
/translation="MPTYTFRCSHCGPFDLTCAISERDAAATCPECRTPARRVFGSVGL
TTFTAGHHRAFDAASASAESPTVVKSIPAGADRPRAPRRNPGLPSLPRY"
gene 3714..3998
/gene="amiD"
/label=amiD
CDS 4003..4644
/codon_start=1
/gene="amiS"
/product="AmiS"
/label=amiS
/note="acetamidase transporter"
/protein_id="ADR02802.1"
/translation="MGGVGLFYVGAVLIIDGLMLLGRISPRGATPLNFFVGGLQVVTPT
VLILQSGGDAAVIFAASGLYLFGFTYLWVAINNVTDWDGEGLGWFSLFVAIAALGYSWH
AFTAEADPAFGVIWLLWAVLWFMLFLLLGLGHDALGPAVGFVAVAEGVITAAVPAFLIV
SGNWETGPLPAAVIAVIGFAAVVLAYPIGRRLAAPSVTNPPPAALAATTR"
gene 4003..4644
/gene="amiS"
/label=amiS
CDS 4802..5614
/label=KanR
/note="aminoglycoside phosphotransferase"
CDS 5757..6104
/codon_start=1
/gene="resD"
/product="ResD"
/label=resD
/note="F plasmid resolvase"
/protein_id="ADR02804.1"
/translation="MERRNRRTGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVP
VTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPE
SDAVAMLKQLS"
gene 5757..6104
/gene="resD"
/label=resD
rep_origin 6499..7113
/label=oriV
/note="origin of replication for the bacterial F plasmid"
rep_origin 7189..7408
/label=ori2
/note="secondary origin of replication for the bacterial F
plasmid; also known as oriS"
CDS 7499..8251
/label=repE
/note="replication initiation protein for the bacterial F
plasmid"
misc_feature 8257..8507
/label=incC
/note="incompatibility region of the bacterial F plasmid"
CDS 8833..10005
/label=sopA
/note="partitioning protein for the bacterial F plasmid"
CDS 10008..10976
/label=sopB
/note="partitioning protein for the bacterial F plasmid"
misc_feature 11052..11525
/label=sopC
/note="centromere-like partitioning region of the bacterial
F plasmid"
misc_feature complement(11785..12183)
/label=cos
/note="lambda cos site; allows packaging into phage lambda
particles"
protein_bind complement(12201..12234)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
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