LOCUS       40924_32923       12530 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pMycoFos, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12530)
  AUTHORS   Ly MA, Liew EF, Le NB, Coleman NV.
  TITLE     Construction and evaluation of pMycoFos, a fosmid shuttle vector for
            Mycobacterium spp. with inducible gene expression and copy number 
            control
  JOURNAL   J. Microbiol. Methods 86 (3), 320-326 (2011)
  PUBMED    21689690
REFERENCE   2  (bases 1 to 12530)
  AUTHORS   Ly MA, Coleman NV.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2010) School of Molecular Bioscience, University 
            of Sydney, Maze Crescent, Darlington, NSW 2006, Australia
REFERENCE   3  (bases 1 to 12530)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2011"; volume: "86"; issue: "3"; pages: 
            "320-326"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-OCT-2010) School of Molecular Bioscience, University of Sydney, 
            Maze Crescent, Darlington, NSW 2006, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      83..1979
                     /note="oriM; derived from pAL5000"
     CDS             complement(2030..3103)
                     /codon_start=1
                     /gene="amiC"
                     /product="AmiC"
                     /label=amiC
                     /note="acetamidase regulator"
                     /protein_id="ADR02799.1"
                     /translation="MQDGEVEFRVGLVIPLQGPAGIFAPSCEAVAELAAKEVNDRGGLQ
                     GRKVTIEVLDGGRPGDDVARTVADRLRGHGLDAVTGWHISAVRNRISPVVRDRIPYVYT
                     SLYEGGERTPGVFCTGETPQIQIAPALAWLRDHFGIRSWCLVGDDYIWPRRSAAAARAY
                     CRDLDLELRREIYVPYGTDDFRAPVRKAIASGAQAVLMLLVGQDAVLFNREFARAGGHD
                     RMARFSPLMEENMLLASGAGSTENLYVAAAYFSSLATAGAMDLMGSYVARYGADAPPLN
                     AMAESCYEGLVALEAIFQRAHSPEIPDLMASAHDVGFDGPRGPMCMRDSQFDQQVYIAS
                     ADGYDFDILDTLTTLDA"
     gene            complement(2030..3103)
                     /gene="amiC"
                     /label=amiC
     CDS             3206..3634
                     /codon_start=1
                     /gene="amiA"
                     /product="AmiA"
                     /label=amiA
                     /note="acetamidase regulator"
                     /protein_id="ADR02800.1"
                     /translation="MAAGQQRRPNLLLPLVRLTHLAESAIERVLADSSLKIEDWRVLDE
                     LAGRRTVPMSDLAQATLITGPTLTRTVDRLVSQGIIYRTADLHDRRRVLVALTPRGRTL
                     RNRLVDAVAEAECAAFESCGLDVDQLRELVDTTSNLTS"
     gene            3206..3634
                     /gene="amiA"
                     /label=amiA
     CDS             3714..3998
                     /codon_start=1
                     /gene="amiD"
                     /product="AmiD"
                     /label=amiD
                     /note="acetamidase regulator"
                     /protein_id="ADR02801.1"
                     /translation="MPTYTFRCSHCGPFDLTCAISERDAAATCPECRTPARRVFGSVGL
                     TTFTAGHHRAFDAASASAESPTVVKSIPAGADRPRAPRRNPGLPSLPRY"
     gene            3714..3998
                     /gene="amiD"
                     /label=amiD
     CDS             4003..4644
                     /codon_start=1
                     /gene="amiS"
                     /product="AmiS"
                     /label=amiS
                     /note="acetamidase transporter"
                     /protein_id="ADR02802.1"
                     /translation="MGGVGLFYVGAVLIIDGLMLLGRISPRGATPLNFFVGGLQVVTPT
                     VLILQSGGDAAVIFAASGLYLFGFTYLWVAINNVTDWDGEGLGWFSLFVAIAALGYSWH
                     AFTAEADPAFGVIWLLWAVLWFMLFLLLGLGHDALGPAVGFVAVAEGVITAAVPAFLIV
                     SGNWETGPLPAAVIAVIGFAAVVLAYPIGRRLAAPSVTNPPPAALAATTR"
     gene            4003..4644
                     /gene="amiS"
                     /label=amiS
     CDS             4802..5614
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             5757..6104
                     /codon_start=1
                     /gene="resD"
                     /product="ResD"
                     /label=resD
                     /note="F plasmid resolvase"
                     /protein_id="ADR02804.1"
                     /translation="MERRNRRTGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVP
                     VTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPE
                     SDAVAMLKQLS"
     gene            5757..6104
                     /gene="resD"
                     /label=resD
     rep_origin      6499..7113
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      7189..7408
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             7499..8251
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    8257..8507
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             8833..10005
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             10008..10976
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    11052..11525
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(11785..12183)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(12201..12234)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcgatctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
       61 cagccgaacg accgagcgca acgcgtgagc ccaccagctc cgtaagttcg ggtgctgtgt
      121 ggctcgtacc cgcgcattca ggcggcaggg ggtctaacgg gtctaaggcg gcgtgtacgg
      181 ccgccacagc ggctcttagc ggcccggaaa cgtcctcgaa acgacgcatg tgttcctcct
      241 ggttggtaca ggtggttggg ggtgctcggc tgtcgctggt gtttcatcat cagggctcga
      301 cgggagagcg ggggagtgtg cagttgtggg gtggcccctc agcgaaatat ctgacttgga
      361 gctcgtgtcg gaccatacac cggtgattaa tcgtggttta ttatcaagcg tgagccacgt
      421 cgccgacgaa tttgagcagc tctggctgcc gtactggtcc ctggcaagcg acgatctgct
      481 cgaggggatc taccgccaaa gccgcgcgtc ggccctaggc cgccggtaca tcgaggcgaa
      541 cccaacagcg ctggcaaacc tgctggtcgt ggacgtagac catccagacg cagcgctccg
      601 agcgctcagc gcccgggggt cccatccgct gcccaacgcg atcgtgggca atcgcgccaa
      661 cggccacgca cacgcagtgt gggcactcaa cgcccctgtt ccacgcaccg aatacgcgcg
      721 gcgtaagccg ctcgcataca tggcggcgtg cgccgaaggc cttcggcgcg ccgtcgatgg
      781 cgaccgcagt tactcaggcc tcatgaccaa aaaccccggc cacatcgcct gggaaacgga
      841 atggctccac tcagatctct acacactcag ccacatcgag gccgagctcg gcgcgaacat
      901 gccaccgccg cgctggcgtc agcagaccac gtacaaagcg gctccgacgc cgctagggcg
      961 gaattgcgca ctgttcgatt ccgtcaggtt gtgggcctat cttcccgccc tcatgcggat
     1021 ctacctgccg acccggaacg tggacggact cggccgcgcg atctatgccg agtgccacgc
     1081 gcgaaacgcc gaatttccgt gcaacgacgt gtgtcccgga ccgctaccgg acagcgaggt
     1141 ccgcgccatc gccaacagca tttggcgttg gatcacaacc aagtcgcgca tttgggcgga
     1201 cgggatcgtg gtctacgagg ccacactcag tgcgcgccat gcggccatct cgcggaaggg
     1261 cgcagcagcg cgcacggcgg cgagcacagt tgcgcggcgc gcaaagtccg cgtcagccat
     1321 ggaggcattg ctatgagcga cggctacagc gacggctaca gcgacggcta caactggcag
     1381 ccgactgtcc gcaaaaagcg gcgcgtgacc gccgccgaag gcgctcgaat caccggacta
     1441 tccgaacgcc acgtcgtccg gctcgtggcg caggaacgca gcgagtggtt cgccgagcag
     1501 gctgcacgcc gcgaacgcat ccgcgcctat cacgacgacg agggccactc ttggccgcaa
     1561 acggccaaac atttcgggct gcatctggac accgttaagc gactcggcta tcgggcgagg
     1621 aaagagcgtg cggcagaaca ggaagcggct caaaaggccc acaacgaagc cgacaatcca
     1681 ccgctgttct aacgcaattg gggagcgggt gtcgcggggg ttccgtgggg ggttccgttg
     1741 caacgggtcg gacaggtaaa agtcctggta gacgctagtt ttctggtttg ggccatgcct
     1801 gtctcgttgc gtgtttcgtt gcgtccgttt tgaataccag ccagacgaga cggggttcta
     1861 cgaatcttgg tcgataccaa gccatttccg ctgaatatcg tggagctcac cgccagaatc
     1921 ggtggttgtg gtgatgtacg tggcgaactc cgttgtagtg cttgtggtgg catccgtggc
     1981 gcggccgcgg taccagatct ttaaatctag ataaagaagt gacgcggtct caagcgtcga
     2041 gcgtcgtcag cgtgtcgagg atgtcgaagt cgtagccgtc ggcgctggcg atgtagacct
     2101 gctggtcgaa ttgactgtcg cgcatacaca tcgggccccg gggcccgtcg aacccgacat
     2161 cgtgcgcgga tgccatcagg tccggtatct cgggggagtg ggcccgctgg aagatggcct
     2221 cgagcgcaac gagaccctcg taacaggatt cggccatcgc gttgagcggt ggcgcgtcgg
     2281 cgccgtagcg ggcgacgtag ctgcccatca ggtccatggc acccgcggtg gccagtgaac
     2341 tgaagtacgc cgcggcgaca tagaggtttt cggtggagcc ggcgccgctg gccagcagca
     2401 tgttctcctc catcagcggg ctgaaccgcg ccatgcggtc gtgcccgccg gcgcgcgcga
     2461 actcgcggtt gaacaacacg gcgtcctggc cgacgagcag catcaacacg gcctgcgccc
     2521 ccgacgcgat ggccttgcgg accggtgcgc ggaaatcgtc ggtgccgtac gggacgtaga
     2581 tctcccgtct gagctcgagg tccagatctc ggcagtacgc gcgggcggcc gcggcggaac
     2641 ggcgcggcca gatgtagtca tcgccgacca ggcaccagga ccggatgccg aagtggtcgc
     2701 gcagccaggc gagcgcgggc gcgatctgga tctgcggtgt ctcgcctgtg cagaacacgc
     2761 ccggtgtgcg ttcaccgccc tcgtacaacg aggtgtagac gtacgggatg cggtcgcgga
     2821 ccaccgggga gatgcggttg cgcacggccg agatgtgcca gccggtcacg gcgtcgagac
     2881 cgtgacctcg caaccggtcg gcgacggtcc gggcgacgtc gtcgccgggc cgtccgccgt
     2941 cgagcacctc gatggtgacc ttgcggccct gcaggccgcc tcggtcgttg acctccttgg
     3001 ccgcgagctc ggccacggcc tcgcacgaag gcgcgaagat tcccgctggc ccttgaagcg
     3061 gaatcaccag cccgacgcgg aactcaacct cgccgtcctg cactccagat caccgtcgat
     3121 cccgtgtagt ctgcgcttca aagctttcta gcagaaataa ttcattctga acagaccccg
     3181 ccgtcgacac gaggagacac ccaccatggc cgccggacag cagcgccgcc ccaacctcct
     3241 gctgccgttg gtgcgtctga cccacctcgc ggagtcggcg atcgaacgcg tgctcgcgga
     3301 ctcgtcgctc aagatcgagg actggcgcgt gctcgacgag ttggccggac ggcgcaccgt
     3361 gcccatgagc gatctcgcgc aggccacgct gatcacgggt ccgactctca ccagaaccgt
     3421 cgatcgcctt gtgtcgcaag ggatcatcta ccggactgcc gatctgcatg accgccggcg
     3481 ggtgctcgtg gcgttgaccc cgcgggggcg gacgctgcgc aaccgcctgg tggacgcggt
     3541 agccgaggcc gagtgtgcgg cttttgaatc gtgcgggctg gacgtcgacc agttgcgcga
     3601 actcgtcgac accacctcga atttgacttc gtaaccaccc gcgcccggcc ggcgttcacc
     3661 cttgactttt attttcatct ggatatattt cgggtgaatg gaaaggggtg accatgccga
     3721 cctacacatt ccgttgttcc cactgcggtc ccttcgatct cacctgcgcg atctccgagc
     3781 gcgatgcggc ggcgacctgt ccggagtgcc ggacgccggc gcgccgggtc ttcggttcgg
     3841 tagggctgac gacattcacc gcgggacatc accgcgcatt cgacgcggcg tccgcgagcg
     3901 ccgaaagtcc cacggtggtg aagtcgattc ccgcaggcgc ggaccgcccg cgggccccgc
     3961 gccgcaatcc cggtctaccg agtctgccga ggtactagcg acatgggtgg cgtcgggctc
     4021 ttctacgtgg gtgcggtgct catcatcgac gggctgatgc tgctgggccg catcagccca
     4081 cgaggcgcaa caccgctgaa cttcttcgtc ggcggactgc aggtggtgac gcctacggtg
     4141 ctgatcctgc agtccggcgg agacgcggcc gtgatcttcg cggcctccgg gctctacctg
     4201 ttcggcttca cctacctgtg ggtggccatc aacaacgtga ccgactggga cggagaaggt
     4261 ctcggatggt tctcgctgtt cgtcgcgatc gccgcactcg gctactcgtg gcacgcgttc
     4321 accgccgagg ccgacccggc gttcggggtg atctggctgc tgtgggcagt gctgtggttc
     4381 atgctgttcc tgctgctcgg cctggggcac gacgcactgg ggcccgccgt cgggttcgtc
     4441 gcggtggccg aaggcgtgat caccgccgcc gtgccggcct tcctgatcgt gtcgggcaac
     4501 tgggaaaccg gcccgctccc cgccgcggtc atcgccgtga tcggttttgc cgcagttgtt
     4561 ctcgcatacc ccatcgggcg ccgtctcgca gcgccgtcag tcaccaaccc tccaccggcc
     4621 gcgctcgcgg ccaccacccg ataagagaaa gggagtccac atctgtaaga attcttaatt
     4681 aagctagcat ttaaatggat ccgtgtctca aaatctctga tgttacattg cacaagataa
     4741 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     4801 tatgagccat attcaacggg aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga
     4861 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     4921 ctatcgcttg tatgggaagc cccatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     4981 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     5041 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     5101 gatccccggg aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     5161 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     5221 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     5281 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     5341 agaaatgcat aatcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     5401 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     5461 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     5521 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     5581 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     5641 acactggcag agcattacgc tgacttgaca tcgattcccg gtatcaacag ggacaccagg
     5701 atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcgcgat aagctcatgg
     5761 agcggcgtaa ccgtcgcaca ggaaggacag agaaagcgcg gatctgggaa gtgacggaca
     5821 gaacggtcag gacctggatt ggggaggcgg ttgccgccgc tgctgctgac ggtgtgacgt
     5881 tctctgttcc ggtcacacca catacgttcc gccattccta tgcgatgcac atgctgtatg
     5941 ccggtatacc gctgaaagtt ctgcaaagcc tgatgggaca taagtccatc agttcaacgg
     6001 aagtctacac gaaggttttt gcgctggatg tggctgcccg gcaccgggtg cagtttgcga
     6061 tgccggagtc tgatgcggtt gcgatgctga aacaattatc ctgagaataa atgccttggc
     6121 ctttatatgg aaatgtggaa ctgagtggat atgctgtttt tgtctgttaa acagagaagc
     6181 tggctgttat ccactgagaa gcgaacgaaa cagtcgggaa aatctcccat tatcgtagag
     6241 atccgcatta ttaatctcag gagcctgtgt agcgtttata ggaagtagtg ttctgtcatg
     6301 atgcctgcaa gcggtaacga aaacgatttg aatatgcctt caggaacaat agaaatcttc
     6361 gtgcggtgtt acgttgaagt ggagcggatt atgtcagcaa tggacagaac aacctaatga
     6421 acacagaacc atgatgtggt ctgtcctttt acagccagta gtgctcgccg cagtcgagcg
     6481 acagggcgaa gccctcggct ggttgccctc gccgctgggc tggcggccgt ctatggccct
     6541 gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg gccgccggcg
     6601 ttgtggatac ctcgcggaaa acttggccct cactgacaga tgaggggcgg acgttgacac
     6661 ttgaggggcc gactcacccg gcgcggcgtt gacagatgag gggcaggctc gatttcggcc
     6721 ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt
     6781 cccacagatg atgtggacaa gcctggggat aagtgccctg cggtattgac acttgagggg
     6841 cgcgactact gacagatgag gggcgcgatc cttgacactt gaggggcaga gtgctgacag
     6901 atgaggggcg cacctattga catttgaggg gctgtccaca ggcagaaaat ccagcatttg
     6961 caagggtttc cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg cttttaaacc
     7021 aatatttata aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc
     7081 gaaggggggt gccccccctt ctcgaaccct cccggtcgag tgagcgagga agcaccaggg
     7141 aacagcactt atatattctg cttacacacg atgcctgaaa aaacttccct tggggttatc
     7201 cacttatcca cggggatatt tttataatta ttttttttat agtttttaga tcttcttttt
     7261 tagagcgcct tgtaggcctt tatccatgct ggttctagag aaggtgttgt gacaaattgc
     7321 cctttcagtg tgacaaatca ccctcaaatg acagtcctgt ctgtgacaaa ttgcccttaa
     7381 ccctgtgaca aattgccctc agaagaagct gttttttcac aaagttatcc ctgcttattg
     7441 actctttttt atttagtgtg acaatctaaa aacttgtcac acttcacatg gatctgtcat
     7501 ggcggaaaca gcggttatca atcacaagaa acgtaaaaat agcccgcgaa tcgtccagtc
     7561 aaacgacctc actgaggcgg catatagtct ctcccgggat caaaaacgta tgctgtatct
     7621 gttcgttgac cagatcagaa aatctgatgg caccctacag gaacatgacg gtatctgcga
     7681 gatccatgtt gctaaatatg ctgaaatatt cggattgacc tctgcggaag ccagtaagga
     7741 tatacggcag gcattgaaga gtttcgcggg gaaggaagtg gttttttatc gccctgaaga
     7801 ggatgccggc gatgaaaaag gctatgaatc ttttccttgg tttatcaaac gtgcgcacag
     7861 tccatccaga gggctttaca gtgtacatat caacccatat ctcattccct tctttatcgg
     7921 gttacagaac cggtttacgc agtttcggct tagtgaaaca aaagaaatca ccaatccgta
     7981 tgccatgcgt ttatacgaat ccctgtgtca gtatcgtaag ccggatggct caggcatcgt
     8041 ctctctgaaa atcgactgga tcatagagcg ttaccagctg cctcaaagtt accagcgtat
     8101 gcctgacttc cgccgccgct tcctgcaggt ctgtgttaat gagatcaaca gcagaactcc
     8161 aatgcgcctc tcatacattg agaaaaagaa aggccgccag acgactcata tcgtattttc
     8221 cttccgcgat atcacttcca tgacgacagg atagtctgag ggttatctgt cacagatttg
     8281 agggtggttc gtcacatttg ttctgaccta ctgagggtaa tttgtcacag ttttgctgtt
     8341 tccttcagcc tgcatggatt ttctcatact ttttgaactg taatttttaa ggaagccaaa
     8401 tttgagggca gtttgtcaca gttgatttcc ttctctttcc cttcgtcatg tgacctgata
     8461 tcgggggtta gttcgtcatc attgatgagg gttgattatc acagtttatt actctgaatt
     8521 ggctatccgc gtgtgtacct ctacctggag tttttcccac ggtggatatt tcttcttgcg
     8581 ctgagcgtaa gagctatctg acagaacagt tcttctttgc ttcctcgcca gttcgctcgc
     8641 tatgctcggt tacacggctg cggcgagcgc tagtgataat aagtgactga ggtatgtgct
     8701 cttcttatct ccttttgtag tgttgctctt attttaaaca actttgcggt tttttgatga
     8761 ctttgcgatt ttgttgttgc tttgcagtaa attgcaagat ttaataaaaa aacgcaaagc
     8821 aatgattaaa ggatgttcag aatgaaactc atggaaacac ttaaccagtg cataaacgct
     8881 ggtcatgaaa tgacgaaggc tatcgccatt gcacagttta atgatgacag cccggaagcg
     8941 aggaaaataa cccggcgctg gagaataggt gaagcagcgg atttagttgg ggtttcttct
     9001 caggctatca gagatgccga gaaagcaggg cgactaccgc acccggatat ggaaattcga
     9061 ggacgggttg agcaacgtgt tggttataca attgaacaaa ttaatcatat gcgtgatgtg
     9121 tttggtacgc gattgcgacg tgctgaagac gtatttccac cggtgatcgg ggttgctgcc
     9181 cataaaggtg gcgtttacaa aacctcagtt tctgttcatc ttgctcagga tctggctctg
     9241 aaggggctac gtgttttgct cgtggaaggt aacgaccccc agggaacagc ctcaatgtat
     9301 cacggatggg taccagatct tcatattcat gcagaagaca ctctcctgcc tttctatctt
     9361 ggggaaaagg acgatgtcac ttatgcaata aagcccactt gctggccggg gcttgacatt
     9421 attccttcct gtctggctct gcaccgtatt gaaactgagt taatgggcaa atttgatgaa
     9481 ggtaaactgc ccaccgatcc acacctgatg ctccgactgg ccattgaaac tgttgctcat
     9541 gactatgatg tcatagttat tgacagcgcg cctaacctgg gtatcggcac gattaatgtc
     9601 gtatgtgctg ctgatgtgct gattgttccc acgcctgctg agttgtttga ctacacctcc
     9661 gcactgcagt ttttcgatat gcttcgtgat ctgctcaaga acgttgatct taaagggttc
     9721 gagcctgatg tacgtatttt gcttaccaaa tacagcaata gtaatggctc tcagtccccg
     9781 tggatggagg agcaaattcg ggatgcctgg ggaagcatgg ttctaaaaaa tgttgtacgt
     9841 gaaacggatg aagttggtaa aggtcagatc cggatgagaa ctgtttttga acaggccatt
     9901 gatcaacgct cttcaactgg tgcctggaga aatgctcttt ctatttggga acctgtctgc
     9961 aatgaaattt tcgatcgtct gattaaacca cgctgggaga ttagataatg aagcgtgcgc
    10021 ctgttattcc aaaacatacg ctcaatactc aaccggttga agatacttcg ttatcgacac
    10081 cagctgcccc gatggtggat tcgttaattg cgcgcgtagg agtaatggct cgcggtaatg
    10141 ccattacttt gcctgtatgt ggtcgggatg tgaagtttac tcttgaagtg ctccggggtg
    10201 atagtgttga gaagacctct cgggtatggt caggtaatga acgtgaccag gagctgctta
    10261 ctgaggacgc actggatgat ctcatccctt cttttctact gactggtcaa cagacaccgg
    10321 cgttcggtcg aagagtatct ggtgtcatag aaattgccga tgggagtcgc cgtcgtaaag
    10381 ctgctgcact taccgaaagt gattatcgtg ttctggttgg cgagctggat gatgagcaga
    10441 tggctgcatt atccagattg ggtaacgatt atcgcccaac aagtgcttat gaacgtggtc
    10501 agcgttatgc aagccgattg cagaatgaat ttgctggaaa tatttctgcg ctggctgatg
    10561 cggaaaatat ttcacgtaag attattaccc gctgtatcaa caccgccaaa ttgcctaaat
    10621 cagttgttgc tcttttttct caccccggtg aactatctgc ccggtcaggt gatgcacttc
    10681 aaaaagcctt tacagataaa gaggaattac ttaagcagca ggcatctaac cttcatgagc
    10741 agaaaaaagc tggggtgata tttgaagctg aagaagttat cactctttta acttctgtgc
    10801 ttaaaacgtc atctgcatca agaactagtt taagctcacg acatcagttt gctcctggag
    10861 cgacagtatt gtataagggc gataaaatgg tgcttaacct ggacaggtct cgtgttccaa
    10921 ctgagtgtat agagaaaatt gaggccattc ttaaggaact tgaaaagcca gcaccctgat
    10981 gcgaccacgt tttagtctac gtttatctgt ctttacttaa tgtcctttgt tacaggccag
    11041 aaagcataac tggcctgaat attctctctg ggcccactgt tccacttgta tcgtcggtct
    11101 gataatcaga ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac
    11161 cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat
    11221 cgtcggtctg ataatcagac tgggaccacg gtcccactcg tatcgtcggt ctgattatta
    11281 gtctgggacc atggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc
    11341 cactcgtatc gtcggtctga ttattagtct ggaaccacgg tcccactcgt atcgtcggtc
    11401 tgattattag tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga
    11461 ccacgatccc actcgtgttg tcggtctgat tatcggtctg ggaccacggt cccacttgta
    11521 ttgtcgatca gactatcagc gtgagactac gattccatca atgcctgtca agggcaagta
    11581 ttgacatgtc gtcgtaacct gtagaacgga gtaacctcgg tgtgcggttg tatgcctgct
    11641 gtggattgct gctgtgtcct gcttatccac aacattttgc gcacggttat gtggacaaaa
    11701 tacctggtta cccaggccgt gccggcacgt taaccgggct gcatccgatg caagtgtgtc
    11761 gctgtcgacg agctcgcgag ctcggacatg aggttgcccc gtattcagtg tcgctgattt
    11821 gtattgtctg aagttgtttt tacgttaagt tgatgcagat caattaatac gatacctgcg
    11881 tcataattga ttatttgacg tggtttgatg gcctccacgc acgttgtgat atgtagatga
    11941 taatcattat cactttacgg gtcctttccg gtgatccgac aggttacggg gcggcgacct
    12001 cgcgggtttt cgctatttat gaaaattttc cggtttaagg cgtttccgtt cttcttcgtc
    12061 ataacttaat gtttttattt aaaataccct ctgaaaagaa aggaaacgac aggtgctgaa
    12121 agcgagcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg
    12181 gaagtccgag ctcatcgcta ataacttcgt atagcataca ttatacgaag ttatattcga
    12241 tgcggccgca aggggttcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
    12301 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
    12361 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcagctgcgc aactgttggg
    12421 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    12481 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttat
//
