Basic Vector Information
- Vector Name:
- pMWO-078
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3377 bp
- Type:
- Expression vector
- Replication origin:
- p15A ori
- Source/Author:
- Obrist MW, Miller VL.
- Promoter:
- PLtetO-1
pMWO-078 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMWO-078 vector Sequence
LOCUS 40924_32858 3377 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pMWO-078, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3377)
AUTHORS Obrist MW, Miller VL.
TITLE Low copy expression vectors for use in Yersinia sp. and related
organisms
JOURNAL Plasmid 68 (1), 33-42 (2012)
PUBMED 22445322
REFERENCE 2 (bases 1 to 3377)
AUTHORS Obrist MW, Miller VL.
TITLE Direct Submission
JOURNAL Submitted (14-DEC-2011) Microbiology and Immunology, University of
North Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen
Jones, CB# 7290, Chapel Hill, NC 27599, USA
REFERENCE 3 (bases 1 to 3377)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3377)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
date: "2012"; volume: "68"; issue: "1"; pages: "33-42"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(14-DEC-2011) Microbiology and Immunology, University of North
Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen Jones,
CB# 7290, Chapel Hill, NC 27599, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Assembly Method :: DNASTAR Lasergene SeqMan Pro v. 7.2.1 (1) Intel
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..3377
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 60..77
/note="MWO-fw; forward sequencing primer"
promoter 75..148
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
misc_feature complement(155..262)
/label=MCS
/note="pBluescript multiple cloning site"
promoter complement(271..289)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(384..405)
/note="MWO-rv-2; reverse sequencing primer"
rep_origin complement(557..1102)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
terminator complement(1245..1331)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(1385..2005)
/label=TetR
/note="tetracycline repressor TetR"
CDS complement(2225..3013)
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
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