LOCUS       40924_32858        3377 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMWO-078, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Low copy expression vectors for use in Yersinia sp. and related 
            organisms
  JOURNAL   Plasmid 68 (1), 33-42 (2012)
  PUBMED    22445322
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Microbiology and Immunology, University of 
            North Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen 
            Jones, CB# 7290, Chapel Hill, NC 27599, USA
REFERENCE   3  (bases 1 to 3377)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3377)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2012"; volume: "68"; issue: "1"; pages: "33-42"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2011) Microbiology and Immunology, University of North 
            Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen Jones, 
            CB# 7290, Chapel Hill, NC 27599, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method :: DNASTAR Lasergene SeqMan Pro v. 7.2.1 (1) Intel 
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..77
                     /note="MWO-fw; forward sequencing primer"
     promoter        75..148
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     misc_feature    complement(155..262)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(271..289)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(384..405)
                     /note="MWO-rv-2; reverse sequencing primer"
     rep_origin      complement(557..1102)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1245..1331)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1385..2005)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             complement(2225..3013)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 gctagcccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
       61 gtcttcacct cgagtcccta tcagtgatag agattgacat ccctatcagt gatagagata
      121 ctgagcacat cagcaggacg cactgaccca attcggtacc gggccccccc tcgaggtcga
      181 cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc
      241 ggccgccacc gcggtggagc tccaattcgc cctatagtga gtcgtattac gacgcgtgga
      301 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
      361 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
      421 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
      481 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
      541 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
      601 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      661 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
      721 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
      781 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
      841 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
      901 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
      961 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     1021 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     1081 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat
     1141 ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt
     1201 gtcagctggg cagcaaaacc cgtaccctag gtctagggcg gcggatttgt cctactcagg
     1261 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
     1321 cgttttattt gatgcctcta gggggagtgg gacaaaattg ctactcagct acctagaatg
     1381 cttaagaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc
     1441 cgaataagaa ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata
     1501 atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt
     1561 gatcttccaa tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat
     1621 tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact
     1681 gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag
     1741 cacatctaaa acttttagcc ttattacgta aaaaatcttg ccagctttcc ccttctaaag
     1801 ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc
     1861 gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt
     1921 tacgggttgt taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca
     1981 ctttactttt atctaatctg gacatatcaa ttcggggcgg gatttcatgg atatgtttct
     2041 ttctgcgaga accagccata tttaaactct tctctcaaat ttatgaatct attatacaga
     2101 aaaattttcc tgaaagcaaa taaatttttt atgatttccc tcgacaattc gcgctaactt
     2161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccgcatg
     2221 cttatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact
     2281 gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa
     2341 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
     2401 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
     2461 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
     2521 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
     2581 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
     2641 ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
     2701 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
     2761 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
     2821 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
     2881 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
     2941 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
     3001 ccgcttccct catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt
     3061 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     3121 gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
     3181 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
     3241 tgcattacag cttacgaacc gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt
     3301 tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct
     3361 ggctggcgaa cgagcgc
//
