Basic Vector Information
- Vector Name:
- pMWO-077
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3329 bp
- Type:
- Expression vector
- Replication origin:
- p15A ori
- Source/Author:
- Obrist MW, Miller VL.
- Promoter:
- PLtetO-1
pMWO-077 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMWO-077 vector Sequence
LOCUS 40924_32853 3329 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector pMWO-077, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3329) AUTHORS Obrist MW, Miller VL. TITLE Low copy expression vectors for use in Yersinia sp. and related organisms JOURNAL Plasmid 68 (1), 33-42 (2012) PUBMED 22445322 REFERENCE 2 (bases 1 to 3329) AUTHORS Obrist MW, Miller VL. TITLE Direct Submission JOURNAL Submitted (14-DEC-2011) Microbiology and Immunology, University of North Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen Jones, CB# 7290, Chapel Hill, NC 27599, USA REFERENCE 3 (bases 1 to 3329) TITLE Direct Submission REFERENCE 4 (bases 1 to 3329) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; date: "2012"; volume: "68"; issue: "1"; pages: "33-42" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (14-DEC-2011) Microbiology and Immunology, University of North Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen Jones, CB# 7290, Chapel Hill, NC 27599, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Assembly Method :: DNASTAR Lasergene SeqMan Pro v. 7.2.1 (1) Intel Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3329 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 60..77 /note="MWO-fw; forward sequencing primer" promoter 75..148 /label=PLtetO-1 promoter /note="modified phage lambda PL promoter with tet operator sites (Lutz and Bujard, 1997)" misc_feature complement(155..262) /label=MCS /note="pBluescript multiple cloning site" promoter complement(271..289) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(384..405) /note="MWO-rv-2; reverse sequencing primer" rep_origin complement(557..1102) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." terminator 1285..1328 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(1385..2005) /label=TetR /note="tetracycline repressor TetR" terminator complement(2258..2344) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(2436..3248) /label=KanR /note="aminoglycoside phosphotransferase"
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