LOCUS       40924_32853        3329 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMWO-077, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Low copy expression vectors for use in Yersinia sp. and related 
            organisms
  JOURNAL   Plasmid 68 (1), 33-42 (2012)
  PUBMED    22445322
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Microbiology and Immunology, University of 
            North Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen 
            Jones, CB# 7290, Chapel Hill, NC 27599, USA
REFERENCE   3  (bases 1 to 3329)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2012"; volume: "68"; issue: "1"; pages: "33-42"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2011) Microbiology and Immunology, University of North 
            Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen Jones, 
            CB# 7290, Chapel Hill, NC 27599, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method :: DNASTAR Lasergene SeqMan Pro v. 7.2.1 (1) Intel 
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..77
                     /note="MWO-fw; forward sequencing primer"
     promoter        75..148
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     misc_feature    complement(155..262)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(271..289)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(384..405)
                     /note="MWO-rv-2; reverse sequencing primer"
     rep_origin      complement(557..1102)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      1285..1328
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1385..2005)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     terminator      complement(2258..2344)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2436..3248)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gctagcccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
       61 gtcttcacct cgagtcccta tcagtgatag agattgacat ccctatcagt gatagagata
      121 ctgagcacat cagcaggacg cactgaccca attcggtacc gggccccccc tcgaggtcga
      181 cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc
      241 ggccgccacc gcggtggagc tccaattcgc cctatagtga gtcgtattac gacgcgtgga
      301 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
      361 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
      421 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
      481 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
      541 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
      601 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      661 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
      721 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
      781 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
      841 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
      901 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
      961 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     1021 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     1081 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat
     1141 ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt
     1201 gtcagctggg cagcaaaacc cgtaccctag gtctagggcg gcggatttgt cctactcagg
     1261 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
     1321 cgttttattt gatgcctcta gggggagtgg gacaaaattg ctactcagct acctagaatg
     1381 cttaagaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc
     1441 cgaataagaa ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata
     1501 atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt
     1561 gatcttccaa tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat
     1621 tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact
     1681 gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag
     1741 cacatctaaa acttttagcc ttattacgta aaaaatcttg ccagctttcc ccttctaaag
     1801 ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc
     1861 gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt
     1921 tacgggttgt taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca
     1981 ctttactttt atctaatctg gacatatcaa ttcggggcgg gatttcatgg atatgtttct
     2041 ttctgcgaga accagccata tttaaactct tctctcaaat ttatgaatct attatacaga
     2101 aaaattttcc tgaaagcaaa taaatttttt atgatttccc tcgacaattc gcgctaactt
     2161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccgcatg
     2221 cggcagcaaa acccgtaccc taggtctagg gcggcggatt tgtcctactc aggagagcgt
     2281 tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
     2341 tttgatgcct ctagggggag tgggacaaaa ttgaaatcaa ataatgattt tattttgacc
     2401 attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag catcaaatga
     2461 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     2521 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     2581 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     2641 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta
     2701 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     2761 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     2821 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     2881 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     2941 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     3001 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     3061 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     3121 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     3181 aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc ccgttgaata
     3241 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgac
     3301 caaaatccct taacgtgagt tttcgttcc
//
