Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V004383 | pMW82 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pMW82
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5129 bp
- Type:
- Promoter-trap vector
- Replication origin:
- ori
- Source/Author:
- Bumann D.
pMW82 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMW82 vector Sequence
LOCUS 40924_32818 5129 bp DNA circular SYN 18-DEC-2018 DEFINITION Promoter-trap vector pMW82, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5129) AUTHORS Bumann D. TITLE In vivo visualization of bacterial colonization, antigen expression, and specific T-cell induction following oral administration of live recombinant Salmonella enterica serovar Typhimurium JOURNAL Infect. Immun. 69 (7), 4618-4626 (2001) PUBMED 11402006 REFERENCE 2 (bases 1 to 5129) AUTHORS Rollenhagen C, Sorensen M, Rizos K, Hurvitz R, Bumann D. TITLE Antigen selection based on expression levels during infection facilitates vaccine development for an intracellular pathogen JOURNAL Proc. Natl. Acad. Sci. U.S.A. 101 (23), 8739-8744 (2004) PUBMED 15173591 REFERENCE 3 (bases 1 to 5129) AUTHORS Wendland M, Bumann D. TITLE Direct Submission JOURNAL Submitted (16-JAN-2007) Molecular Biology, Max-Planck-Institute for Infection Biology, Chartieplatz 1, Berlin D-10117, Germany REFERENCE 4 (bases 1 to 5129) TITLE Direct Submission REFERENCE 5 (bases 1 to 5129) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Infect. Immun."; date: "2001"; volume: "69"; issue: "7"; pages: "4618-4626" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Proc. Natl. Acad. Sci. U.S.A."; date: "2004"; volume: "101"; issue: "23"; pages: "8739-8744" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (16-JAN-2007) Molecular Biology, Max-Planck-Institute for Infection Biology, Chartieplatz 1, Berlin D-10117, Germany" COMMENT SGRef: number: 4; type: "Journal Article" FEATURES Location/Qualifiers source 1..5129 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 32..745 /codon_start=1 /label=yeGFP /note="yeast-enhanced green fluorescent protein" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" terminator 1038..1124 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1216..1243 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 1262..1353 /label=AmpR promoter CDS 1354..2211 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2385..2973 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(3159..3299) /label=bom /note="basis of mobility region from pBR322" CDS complement(3404..3592) /codon_start=1 /label=rop /note="Rop protein, which maintains plasmids at low copy number" /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL"