LOCUS       40924_32818        5129 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter-trap vector pMW82, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Bumann D.
  TITLE     In vivo visualization of bacterial colonization, antigen expression,
            and specific T-cell induction following oral administration of live 
            recombinant Salmonella enterica serovar Typhimurium
  JOURNAL   Infect. Immun. 69 (7), 4618-4626 (2001)
  PUBMED    11402006
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Rollenhagen C, Sorensen M, Rizos K, Hurvitz R, Bumann D.
  TITLE     Antigen selection based on expression levels during infection 
            facilitates vaccine development for an intracellular pathogen
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 101 (23), 8739-8744 (2004)
  PUBMED    15173591
REFERENCE   3  (bases 1 to 5129)
  AUTHORS   Wendland M, Bumann D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2007) Molecular Biology, Max-Planck-Institute for 
            Infection Biology, Chartieplatz 1, Berlin D-10117, Germany
REFERENCE   4  (bases 1 to 5129)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5129)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect. 
            Immun."; date: "2001"; volume: "69"; issue: "7"; pages: "4618-4626"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2004"; volume: "101"; issue: "23"; pages:
            "8739-8744"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (16-JAN-2007) Molecular Biology, Max-Planck-Institute for Infection 
            Biology, Chartieplatz 1, Berlin D-10117, Germany"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             32..745
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1038..1124
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1216..1243
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1262..1353
                     /label=AmpR promoter
     CDS             1354..2211
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2385..2973
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3159..3299)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3404..3592)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gatcctctag atttaagaag gagatataca tatgagtaaa ggagaagaac ttttcactgg
       61 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      121 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      181 tggaaaacta cctgttccat ggccaacact tgtcactact ttcgcgtatg gtcttcaatg
      241 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
      301 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      361 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      421 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
      481 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
      541 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
      601 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
      661 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
      721 acatggcatg gatgaactat acaaagaatc tctgaaaatc tctcaggctg ttcacgctgc
      781 tcacgctgaa atcaacgaag ctggtcgtga agtagtaggt taactgcagc caagcttctg
      841 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
      901 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
      961 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
     1021 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1081 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     1141 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     1201 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
     1261 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1321 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1381 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1441 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1501 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1561 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     1621 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1681 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1741 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1801 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1861 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1921 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1981 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2041 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2101 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2161 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2221 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2281 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2341 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2401 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2461 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2521 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2581 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2641 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2701 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2761 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2821 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2881 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2941 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3001 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3061 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3121 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     3181 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     3241 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     3301 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3361 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     3421 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
     3481 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     3541 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc acttgatgcc
     3601 tccgtgtaag ggggaatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     3661 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     3721 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg
     3781 cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat
     3841 ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa
     3901 accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca
     3961 cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag
     4021 ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc
     4081 cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg
     4141 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt
     4201 gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca
     4261 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac
     4321 ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc
     4381 gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct
     4441 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
     4501 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc
     4561 gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
     4621 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
     4681 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
     4741 acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg
     4801 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc
     4861 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     4921 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
     4981 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     5041 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     5101 atgccggcca cgatgcgtcc ggcgtagag
//
