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pMV261-EO6 vector (V004399)

Basic Vector Information

Vector Name:
pMV261-EO6
Antibiotic Resistance:
Kanamycin
Length:
5693 bp
Type:
Shuttle vector
Replication origin:
ori
Source/Author:
Dennehy ME, Bourn W, Steele AD, Williamson A-L.

pMV261-EO6 vector Vector Map

pMV261-EO65693 bp60012001800240030003600420048005400KanRoriMycobacterial OriM region derived from pAL5000derived from Mycobacterium bovis hsp60 promoter of GenBank Accession Number M17705Intermediate capsid protein VP6rrnB T1 terminator

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMV261-EO6 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V004399                 5693 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004399
VERSION     V004399
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5693)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5693)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-JUL-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      2028..3927
                     /label="Mycobacterial OriM region derived from pAL5000"
                     /note="Mycobacterial OriM region derived from pAL5000"
     regulatory      3950..4335
                     /note="derived from Mycobacterium bovis hsp60 promoter of
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     CDS             4362..5552
                     /note="Intermediate capsid protein VP6 from Rotavirus A
                     (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]).
                     Accession#: B2BN53"
                     /label="Intermediate capsid protein VP6"
     terminator      5611..5657
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"

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