LOCUS       V004399                 5693 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004399
VERSION     V004399
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5693)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5693)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-JUL-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      2028..3927
                     /label="Mycobacterial OriM region derived from pAL5000"
                     /note="Mycobacterial OriM region derived from pAL5000"
     regulatory      3950..4335
                     /note="derived from Mycobacterium bovis hsp60 promoter of
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     CDS             4362..5552
                     /note="Intermediate capsid protein VP6 from Rotavirus A
                     (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]).
                     Accession#: B2BN53"
                     /label="Intermediate capsid protein VP6"
     terminator      5611..5657
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
       61 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      121 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      181 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      241 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      301 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      361 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      421 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      481 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      541 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      601 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      661 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      721 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      781 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      841 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
      901 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
      961 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1021 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1081 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1141 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1201 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     1681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1981 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcacgcgtg agcccaccag
     2041 ctccgtaagt tcgggtgctg tgtggctcgt acccgcgcat tcaggcggca gggggtctaa
     2101 cgggtctaag gcggcgtgta cggccgccac agcggctctt agcggcccgg aaacgtcctc
     2161 gaaacgacgc atgtgttcct cctggttggt acaggtggtt gggggtgctc ggctgtcgct
     2221 ggtgtttcat catcagggct cgacgggaga gcgggggagt gtgcagttgt ggggtggccc
     2281 ctcagcgaaa tatctgactt ggagctcgtg tcggaccata caccggtgat taatcgtggt
     2341 ctactaccaa gcgtgagcca cgtcgccgac gaatttgagc agctctggct gccgtactgg
     2401 ccgctggcaa gcgacgatct gctcgagggg atctaccgcc aaagccgcgc gtcggcccta
     2461 ggccgccggt acatcgaggc gaacccaaca gcgctggcaa acctgctggt cgtggacgta
     2521 gaccatccag acgcagcgct ccgagcgctc agcgcccggg ggtcccatcc gctgcccaac
     2581 gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag tgtgggcact caacgcccct
     2641 gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat acatggcggc gtgcgccgaa
     2701 ggccttcggc gcgccgtcga tggcgaccgc agttactcag gcctcatgac caaaaacccc
     2761 ggccacatcg cctgggaaac ggaatggctc cactcagatc tctacacact cagccacatc
     2821 gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc gtcagcagac cacgtacaaa
     2881 gcggctccga cgccgctagg gcggaattgc gcactgttcg attccgtcag gttgtgggcc
     2941 tatcgtcccg ccctcatgcg gatctacctg ccgacccgga acgtggacgg actcggccgc
     3001 gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc cgtgcaacga cgtgtgtccc
     3061 ggaccgctac cggacagcga ggtccgcgcc atcgccaaca gcatttggcg ttggatcaca
     3121 accaagtcgc gcatttgggc ggacgggatc gtggtctacg aggccacact cagtgcgcgc
     3181 cagtcggcca tctcgcggaa gggcgcagca gcgcgcacgg cggcgagcac agttgcgcgg
     3241 cgcgcaaagt ccgcgtcagc catgcatgga ggcattgcta tgagcgacgg ctacagcgac
     3301 ggctacagcg acggctacaa ccggcagccg actgtccgca aaaagcggcg cgtgaccgcc
     3361 gccgaaggcg ctcgaatcac cggactatcc gaacgccacg tcgtccggct cgtggcgcag
     3421 gaacgcagcg agtggctcgc cgagcaggct gcacgccgcg aacgcatccg cgcctatcac
     3481 gacgacgagg gccactcttg gccgcaaacg gccaaacatt tcgggctgca tctggacacc
     3541 gttaagcgac tcggctatcg ggcgaggaaa gagcgtgcgg cagaacagga agcggctcaa
     3601 aaggcccaca acgaagccga caatccaccg ctgttctaac gcaattgggg agcgggtgtc
     3661 gcgggggttc cgtggggggt tccgttgcaa cgggtcggac aggtaaaagt cctggtagac
     3721 gctagttttc tggtttgggc catgcctgtc tcgttgcgtg tttcgttgcg tccgttttga
     3781 ataccagcca gacgagacgg ggttctacga atcttggtcg ataccaagcc atttccgctg
     3841 aatatcgtgg agctcaccgc cagaatcggt ggttgtggtg atgtacgtgg cgaactccgt
     3901 tgtagtgctt gtggtggcat ccgtggcgcg gccgcggtac cagatcttta aatctagacg
     3961 gtgaccacaa cgacgcgccc gctttgatcg gggacgtctg cggccgacca tttacgggtc
     4021 ttgttgtcgt tggcggtcat gggccgaaca tactcacccg gatcggaggg ccgaggacaa
     4081 ggtcgaacga ggggcatgac ccggtgcggg gcttcttgca ctcggcatag gcgagtgcta
     4141 agaataacgt tggcactcgc gaccggtgag tcgtaggtcg ggacggtgag gccaggcccg
     4201 tcgtcgcagc gagtggcagc gaggacaact tgagccgtcc gtcgcgggca ctgcgcccgg
     4261 ccagcgtaag tagcggggtt gccgtcaccc ggtgaccccc ggtttcatcc ccgatccgga
     4321 ggaatcactt cgcaatggcc aagacaattg cggatcccaa aatggatgtg ctgtactcca
     4381 tctcgagaac actgaaggat gctagggata aaattgttga gggtacgttg tactctaacg
     4441 ttagtgatct tattcagcag ttcaatcaga tgctggtcac aatgaatgga aatgaatttc
     4501 aaacgggggg aattggtaac ttgccacttc gtaattggaa ctttgatttt ggactactag
     4561 gtactacgct attaaatcta gacgcaaact acgttgaatc agccagaacc acaattgatt
     4621 attttgttga tttcatagat aatgtatgta tggatgaaat ggtgcgagag tcgcaaagga
     4681 atggaatagc accacaatca gatgccttgc ggaagttatc aggagttaag ttcagacgaa
     4741 taaactttaa caattcatca gagtatattg agaactggaa ccttcagaac cgcaggcaaa
     4801 ggacgggatt cacgtttcac aaaccgaaca tatttccata ttcggcatca ttcacattga
     4861 ataggtcgca gccgcagcat gacaacttaa tgggcacaat gtggctaaac gctgggtcag
     4921 aaatacaggt tgcaggattt gactattcat gcgcaattaa cgctccagct aacattcaac
     4981 aatttgaaca tatagtgcaa ttacggaggg tattgactac tgcaaccatt acgctacttc
     5041 cggatgcaga gaggtttagt ttcccaagag ttattaactc agcagacgga gcaactacgt
     5101 ggtatttcaa cccagtaata ttgcgtccaa ataatgtaga agttgaattc ttattgaatg
     5161 gtcagatcat caatacttac caagctagat tcggtaccat cgtcgcaaga aattttgata
     5221 ccatacgcct ttcttttcag ctgatgagac caccaaacat gacaccagca gtggctgcac
     5281 ttttcccgaa tgctcaaccg ttcgagcatc atgcaacagt cggactgacg cttaggattg
     5341 actcagcaat ttgtgaatca gttcttgcag acgcaagtga aactatgttg gctaatgtga
     5401 cgtcagtcag acaagaatac gcaataccag taggaccagt atttccacca ggaatgaatt
     5461 ggaccgacct cattactaat tattcaccat caagggagga caatctgcaa cgtgtgttta
     5521 cagtagcttc cattagaagc atgttggtaa agtgagcgaa gcttatcgat gtcgacgtag
     5581 ttaactagcg tacgatcgac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
     5641 tgggcctttc gttttatctg ttgtttgtcc ggccatcatg gccgcggtga tca
//
