Basic Vector Information
- Vector Name:
- pMU125
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9445 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Bahl CD.
- Promoter:
- tet
pMU125 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMU125 vector Sequence
LOCUS 40924_32688 9445 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMU125, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9445)
AUTHORS Bahl CD.
TITLE Sequencing commonly used, cryptic plasmids from Acinetobacter
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9445)
AUTHORS Bahl CD.
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2014) Biochemistry, University of Washington, Box
351655, Room 420, Seattle, WA 98195-1655, USA
REFERENCE 3 (bases 1 to 9445)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9445)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-JUN-2014) Biochemistry, University of Washington, Box 351655,
Room 420, Seattle, WA 98195-1655, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9445
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature complement(110..250)
/label=bom
/note="basis of mobility region from pBR322"
rep_origin 1619..2955
/label=pWH1266_ori
/note="pWH1266_ori"
CDS complement(5732..6400)
/codon_start=1
/product="TetA"
/label=TetA
/note="class C tetracycline resistance protein"
/protein_id="AIO09752.1"
/translation="MSRPAGIRYQAYRYRRPMPLRAFNPVSSFRWARGMTIVAALMTVF
FIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQ
AIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQ
GSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
"
CDS complement(6404..7117)
/codon_start=1
/label=yeGFP
/note="yeast-enhanced green fluorescent protein"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
promoter complement(7486..7514)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
promoter 7627..7731
/label=AmpR promoter
CDS 7732..8589
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 8763..9351
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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