LOCUS       40924_32688        9445 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMU125, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Bahl CD.
  TITLE     Sequencing commonly used, cryptic plasmids from Acinetobacter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   Bahl CD.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2014) Biochemistry, University of Washington, Box 
            351655, Room 420, Seattle, WA 98195-1655, USA
REFERENCE   3  (bases 1 to 9445)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9445)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JUN-2014) Biochemistry, University of Washington, Box 351655, 
            Room 420, Seattle, WA 98195-1655, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(110..250)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      1619..2955
                     /label=pWH1266_ori
                     /note="pWH1266_ori"
     CDS             complement(5732..6400)
                     /codon_start=1
                     /product="TetA"
                     /label=TetA
                     /note="class C tetracycline resistance protein"
                     /protein_id="AIO09752.1"
                     /translation="MSRPAGIRYQAYRYRRPMPLRAFNPVSSFRWARGMTIVAALMTVF
                     FIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQ
                     AIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQ
                     GSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
                     "
     CDS             complement(6404..7117)
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(7486..7514)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        7627..7731
                     /label=AmpR promoter
     CDS             7732..8589
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8763..9351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
       61 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
      121 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
      181 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
      241 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
      301 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
      361 ttcaccgtca tcaccgaaac gcgcgaggca gctggaggat gccgtagacc aagcccccgc
      421 agtctatgcc accgcacaac aggcactata cgaccaccaa caacaccgcc aaacgcttat
      481 agatgtgtat ttgattgaag taaaaacgga tgagaagcga gcagagcata gattttacaa
      541 ggctttgcaa gacaatatcg accgcttgca gcttgataaa gtgatcaaag agggacgagc
      601 agctacagca ctgctaaggg agatcggaga gccactgccg ccaccagtga aagtgaaaac
      661 aggttggttt agtgctgatt attacggctt tgaggacatg ctcacacgtt gggataatga
      721 tcaatctagc ctttccattt atcacagaga gataaaagag gagcaacagg aacagcaacg
      781 aaaacaacag cagatagaaa gagaaactaa acaaaaacag gaagatgaca aaaagaaacg
      841 gcaggactat gaaagatatt tatcaaaata cggctatgtc aaagatgctg agtatgacca
      901 tatcagcgac catgttgctt cagacatttc catgtttaca cgtcttcagg cgcaggcatg
      961 ggcaagtaac gatatgcaag agtacataag gtgttcaaag caaaaatacg agcagatcag
     1021 cgacagaata gcctatgagc gtgacttgaa aaaaatcggg caattgagcc gtatcctgga
     1081 taaagacaga caggcacttg ctgatatttt accgcagttg atgccttact atgagcagat
     1141 gcaacaggct gttaataaac ggaaaatttt acttgagaat gagcgacagc catcatttaa
     1201 accacgttat gaccaagaac agcctaagcc gaaaaaagac aatgacctaa ctttttaatg
     1261 tgttacaata gcattcaaat gtaacacatt ggagtgaaaa acatgacaga agcgactttt
     1321 acctttcggg tagaccatga cctaaagcaa gaattttcaa gccttgctaa gactgttgat
     1381 cggtcggggg cgcagctaat ccgtgacttc atgcgtgact ttgttaagaa gcaacaggaa
     1441 gccgcagact atgacaaatg gttcaagcag caggtacaga tcggcttaaa tgaagccaat
     1501 gcaggcaagt tgattccaca tgaggacgta aaggcagagt ttgccgctag acgtgctgca
     1561 acattggcga aactggcagc gaagaatgaa gattgagtgg actgaaacag ctcgccaaga
     1621 tcgtagaaat atctatgatt atcttgaaga acgcaaccct atagcagcta ttgaaattga
     1681 tgatttaatt gaagaaaaga cagatttact tgttgataat cgactgatgg ggcgcacagg
     1741 cagacagaaa gatactaggg agttagtgat acatccgcat tatgtggttg tatatgacat
     1801 cactgatata atacggatac tcagagtgct acacacatcg caggagtggt catgacttac
     1861 tcatgtactt tggattattt agtgttataa aatcctgatt tataaatttt ttttgttaaa
     1921 aaagataaaa gccccttgca attgcttggg gctttaccgt aatttatggg gtacagatct
     1981 tcgatactga catatcggca atcgaaagca ttaaggtttg acgaccgcta atgatttcac
     2041 cacaggggct taatgtacct gtcttaaatt ctaaggtttt aactcgcttt gtcaagcata
     2101 gaccccaaaa atttagccaa tgtctgtaac tcaatctgtc catgtgtggg tgatgaggta
     2161 cagtgacgct agcacacatc ggaaaaacgc tattactagg ggaactgaac agagtagcgg
     2221 acgcaatgag tagtcattta attggcggtt atgagcgtgt tcaggcggtg ctatcaatcg
     2281 taatcataac agtggcagct tgatacagtg atgtcatccc tgatgcgaaa gcgaccgacc
     2341 gacggtacat cgaatgggaa tactttaggg tgatttttaa gaatcgctct agggtgagta
     2401 tttcccattc agctctgctc cctccctctg gtactttaat caaaagcact actaaacata
     2461 tgtttttaaa taaaaaatat tgatatagag ataatattag taagaataat taaacaattg
     2521 aatatagata aatcattgtt aaataaagat taattattaa aatgaatgta tacttatata
     2581 taaatcaatg atttaaaata tttgataaag aaaacttttc aaaaaaaata taattgagat
     2641 tgtgtcattt cggtcaattc ttaatatgtt ccacgcaagt tttagctatg gtgctaaaca
     2701 gaaatttgct gaaaaagaac ttttcactga actggttaaa atgtaagcag cctgagagcc
     2761 gccaaaaatt ttaaaaacaa accgccttaa tcatcttcaa aaaatacctc taaaacctca
     2821 ccatttgcgt tttaagaccc atatttcatc ctgcccttat gttcccatgc tgatagctat
     2881 aaagtgtctg taatcgcttc ctatgacgtt ctaggctgtt gataactttt ggaacaacgc
     2941 aaaatgttaa aatccgatca ttttttaacc tagttatttt cgttacaggg taactttggt
     3001 agtattattt caatatttag ttgttacagg ataacaaaat atatgttaca gggtaattaa
     3061 tttagcgtta caggataact atagttacag ggtaacgtta aatagttatc ctgtaattta
     3121 aaaatagttg caggatagca taaattttta caaataaatt ttataaaaag ttatcctgta
     3181 actaaaaaca atgttatcct gtaactatga tatactatat gggagttaaa gacatgaaag
     3241 acccgaacga tcagaaaaca caggacatgc taaaagagcc gccaaaaacc aatgccgaac
     3301 gacaaaaagc atatcgtgaa aaacgcaaaa gccttgatag tcaacgtttg gaggtgttta
     3361 tagataaggg tgtgtcggat atgcttgcgg acatggtggg cgcagcaggg gagagccaaa
     3421 aagccatttt gaccgcattg attgagaaag agtataagcg gctgtatgca gtgaaaaata
     3481 gcaagctaaa aaagtacatt gattcaaaaa gtaatgcaga taaaaaagaa accctcgatt
     3541 tatgagggtt ttttataact actaatgcct atgaaaataa gtgcttatca ttttctctaa
     3601 tgtacccaat aaaatcgggg ttttgttcca caagtttctt gatgtcatta tcatgtttct
     3661 tcagtaagta agccccgatc aggatcttgc gttttgcatc gtctgacctt gcctgttttt
     3721 gctctcgacc tttcaccaca gcgtcagcta gatcctttct tgccttttcc tgacgttcca
     3781 atttttcctt ttcttcatcg agcatttgac ccaattccat agctgttttt ttgaccattt
     3841 ttaatgcctg tgtctcaacc tattttccga agtatgaacc atcatcaatg tgaaaagtgt
     3901 tctacttcaa aaactcgact tacatcaaga tccgagcgca gcgagcaaaa tcaaaaaaca
     3961 aagtcaaagc cttattgctc ttgcttttgc ttttagcctc gcagagttcc cgaagggcgc
     4021 acttacgcaa aatttttgct acgccaaatt ttgcaagtac ggtcagggaa accccgacac
     4081 cccaaccgcc caaaacttgg ggcggctgtt aataaacagg tgatgaaaat ggcaatttac
     4141 cattgtgaaa tgcagaacat ttcgaggtca gatggtcgct caatcgtggc atgtgcagca
     4201 taccgagcag gcgaaaaatt gtactgtgat acgtacggaa aagagcagga ctacaccaaa
     4261 aaaacaggca ttgaatacac ccaaattttt gccccaactg gggcaagtcc tgacatgtta
     4321 gatcgtcaaa ccctatggaa tcgggtagag caatccgaac taaaaaagaa cggtgacatc
     4381 aaacaggagg caagattagc aaaggaagta gagatagcat tgccgcatga actggataag
     4441 acacagcgtc aggcacttgt taccgagttg tgccagtcct tagttaaagc ctatggagtg
     4501 gcggtggacg tagcgatcca tgcccctcat gtgcatgggg gaagtgatga aagaaaccat
     4561 cacgcccaca tactaatgac aacaagaagc cttacaccgc aagggctagg gaagaaagtc
     4621 agggagctag atcagaactc aacgctgaaa aaaatcaggg aaaaagtggc ggatctgacg
     4681 aacgtccacc tagagcgagc agggcttgag gtgagagttg atcaccgcag tcataaggat
     4741 cgtggactag acatcgagcc gacattcaag gagggacttg aagccaccca cgccaagcgc
     4801 aggggggagg aactggagat cgtcaagcgt aatgatgaaa tcaaaaagcg caataaaaaa
     4861 gctgaaaggc ttgatatgtc tatcattgag acgagcaaca gagtagagca attgacactt
     4921 gaacagcagc aacagcagca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     4981 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     5041 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     5101 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     5161 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     5221 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     5281 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     5341 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     5401 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     5461 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     5521 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc
     5581 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg
     5641 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg
     5701 aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac
     5761 cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc
     5821 cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg
     5881 aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta
     5941 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa
     6001 tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg
     6061 cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac
     6121 cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga
     6181 tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca
     6241 cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc
     6301 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacggt
     6361 atcgataagc ttgatatcga attcctgcag gtctggacat ttatttgtat agttcatcca
     6421 tgccatgtgt aatcccagca gctgttacaa actcaagaag gaccatgtgg tctctctttt
     6481 cgttgggatc tttcgaaagg gcagattgtg tggacaggta atggttgtct ggtaaaagga
     6541 cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga acgcttccat
     6601 cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc attcttttgt ttgtctgcca
     6661 tgatgtatac attgtgtgag ttatagttgt attccaattt gtgtccaaga atgtttccat
     6721 cttctttaaa atcaatacct tttaactcga ttctattaac aagggtatca ccttcaaact
     6781 tgacttcagc acgtgtcttg tagttcccgt catctttgaa aaatatagtt ctttcctgta
     6841 cataaccttc gggcatggca ctcttgaaaa agtcatgctg tttcatatga tctgggtatc
     6901 tcgcaaagca ttgaacacca taaccgaaag tagtgacaag tgttggccat ggaacaggta
     6961 gttttccagt agtgcaaata aatttaaggg taagttttcc gtatgttgca tcaccttcac
     7021 cctctccact gacagaaaat ttgtgcccat taacatcacc atctaattca acaagaattg
     7081 ggacaactcc agtgaaaagt tcttctcctt tactcatatg tatatctcct tcttaaatct
     7141 agaggatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta
     7201 gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg
     7261 cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc
     7321 tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc
     7381 ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac
     7441 acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga
     7501 tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt
     7561 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7621 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7681 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7741 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7801 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7861 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7921 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     7981 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8041 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8101 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8161 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8221 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     8281 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8341 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8401 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8461 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8521 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8581 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8641 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8701 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8761 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8821 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8881 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     8941 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9001 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9061 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9121 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9181 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9241 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9301 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9361 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9421 gcgttatccc ctgattctgt ggata
//
