pMTet1 vector (V004448)

LOCUS       V004448                 5086 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004448
VERSION     V004448
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Matin MM, Hornby DP.
  TITLE     A positive selection vector combining tetracycline resistance that
            eliminates the need for bacterial plating
  JOURNAL   Anal. Biochem. 278 (1), 46-51 (2000)
   PUBMED   10640352
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Matin MM, Hornby DP.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2001) Molecular Biology, Krebs Institute, Firth
            Court, Western Bank, Sheffield S10 2TN, UK
REFERENCE   3  (bases 1 to 5086)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5086)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal.
            Biochem."; date: "2000"; volume: "278"; issue: "1"; pages: "46-51"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-OCT-2001) Molecular Biology, Krebs Institute, Firth Court,
            Western Bank, Sheffield S10 2TN, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             92..1279
                     /label="TcR"
                     /note="tetracycline efflux protein"
     rep_origin      2799..3387
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3675..3696
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3711..3741
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3749..3765
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3773..3789
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3829..5082
                     /gene="mspIM"
                     /label="Type II methyltransferase M.MspI"
                     /note="Type II methyltransferase M.MspI from Moraxella sp.
                     Accession#: P11408"