LOCUS       V004448                 5086 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004448
VERSION     V004448
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Matin MM, Hornby DP.
  TITLE     A positive selection vector combining tetracycline resistance that
            eliminates the need for bacterial plating
  JOURNAL   Anal. Biochem. 278 (1), 46-51 (2000)
   PUBMED   10640352
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Matin MM, Hornby DP.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2001) Molecular Biology, Krebs Institute, Firth
            Court, Western Bank, Sheffield S10 2TN, UK
REFERENCE   3  (bases 1 to 5086)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5086)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal.
            Biochem."; date: "2000"; volume: "278"; issue: "1"; pages: "46-51"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-OCT-2001) Molecular Biology, Krebs Institute, Firth Court,
            Western Bank, Sheffield S10 2TN, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             92..1279
                     /label="TcR"
                     /note="tetracycline efflux protein"
     rep_origin      2799..3387
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3675..3696
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3711..3741
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3749..3765
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3773..3789
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3829..5082
                     /gene="mspIM"
                     /label="Type II methyltransferase M.MspI"
                     /note="Type II methyltransferase M.MspI from Moraxella sp.
                     Accession#: P11408"
ORIGIN
        1 aattctcatg tttgacagct tatcatcgat aagctagctt taatgcggta gtttatcaca
       61 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
      121 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
      181 cctcttgcgg gatatagtcc attccgacag catcgccagt cactatggcg tgctgctagc
      241 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      301 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      361 ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg
      421 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      481 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      541 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
      601 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgacg
      661 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      721 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      781 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
      841 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
      901 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
      961 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1021 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1081 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac
     1141 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1201 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1261 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1321 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg
     1381 cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc
     1441 gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
     1501 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
     1561 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
     1621 gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc
     1681 atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg
     1741 cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc
     1801 tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc
     1861 tctcgtttca tcggtatcat tacccccatg aacagaaatt cccccttaca cggaggcatc
     1921 aagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat
     1981 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat
     2041 cgcttcacga ccacgctgat gagctttacc gcagactcac cagtcacaga aaagcatctt
     2101 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2161 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2221 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2281 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2341 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2401 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2461 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     2521 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2581 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2641 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2701 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2761 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2821 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2881 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2941 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3001 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3061 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3121 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3181 cagcgtgagc tttgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3241 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3301 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3361 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3421 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3481 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3541 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3601 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3661 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     3721 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     3781 agctatgacc atgattacgc caagctgctg tgcggccgca atggatccat gaaacctgaa
     3841 atattgaaat tgattcgtag taaattggat ctaactcaaa agcaagcatc tgaaattatt
     3901 gaagttagtg ataaagcatg gcaacagtgg gaatcaggta aaacagaaat gcatcctgct
     3961 tattactctt ttctccaaga aaaacttaaa gataagatta actttgagga actgagcgct
     4021 caaaaaacac tccaaaagaa gatattcgat aaatacaatc agaatcaaat tactaaaaat
     4081 gcggaagaat tagctgaaat tactcatatt gaagaaagaa aagatgctta tagcagtgat
     4141 ttcaaattta ttgatttatt ttctggtatc ggtggaataa ggcaatcatt cgaagtgaat
     4201 ggcggaaaat gcgtcttttc gtctgaaata gatccctttg caaaatttac atattataca
     4261 aattttggtg tagtcccttt tggagatatt acaaaagttg aggctacaac aattccggag
     4321 catgacattt tatgtgcagg atttccgggc cagccatgga gccatatggg taaaagagaa
     4381 ggctttgaac atccaactca aggaacaatg ttccatgaaa ttgttcgtat cattgaaaca
     4441 aaaaaaaccc cagttttatt tctagaaaat gttcctggtc tcattaatca tgatgacgga
     4501 aatacattaa aagtcatcat tgaaacacta gaagatatgg gctacaaagt tcatcatacg
     4561 gtactagacg ctagtcattt tggtatccca caaaaacgta agcgttttta cttagtagct
     4621 ttcctaaatc aaaatattca ctttgagttt cctaaacctc caatgatttc taaagatatc
     4681 ggtgaagttt tagaaagcga tgtaactgga tatagcattt cagagcattt acaaaaaagt
     4741 tatctcttta aaaaagatga tggtaaacct tctttaattg acaaaaatac gactggggca
     4801 gttaaaacat tagtttctac ctatcacaaa attcaacgat taacaggtac ttttgttaag
     4861 gatggagaaa cagctatccg tcttttaaca acgaatgaat gcaaagctat tatgggtttt
     4921 ccaaaagatt ttgttattcc tgtatcaaga actcagatgt accgtcaaat gggtaactct
     4981 gtcgtagttc cggtggttac aaaaattgca gaacagatta gtttggcact aaaaactgtt
     5041 aaccaacaat ccccgcaaga aaactttgaa ttagaactcg tttaag
//
