pMTCbLCVA.008 vector (V004449)

LOCUS       V004449                 5702 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004449
VERSION     V004449
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Turnage MA, Muangsan N, Peele CG, Robertson D.
  TITLE     Geminivirus-based vectors for gene silencing in Arabidopsis
  JOURNAL   Plant J. 30 (1), 107-114 (2002)
   PUBMED   11967097
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Turnage MA, Muangsan N, Peele CG, Robertson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2003) Botany, North Carolina State University, Box
            7612, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 5702)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5702)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
            date: "2002"; volume: "30"; issue: "1"; pages: "107-114"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-APR-2003) Botany, North Carolina State University, Box 7612,
            Raleigh, NC 27695, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..858)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(859..963)
                     /label="AmpR promoter"
     rep_origin      complement(989..1444)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1586..1602
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1612..1630
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2065..3111
                     /note="Replication-associated protein from Cabbage leaf
                     curl virus (isolate Jamaica). Accession#: Q96704"
                     /label="Replication-associated protein"
     CDS             3056..3442
                     /note="Transcriptional activator protein from Cabbage leaf
                     curl virus (isolate Jamaica). Accession#: Q96703"
                     /label="Transcriptional activator protein"
     misc_feature    3652..4024
                     /note="derived from GenBank Accession Number AY140022; 360
                     bp of Arabidopsis thaliana ChlI replaces Cabbage leaf curl
                     virus coat protein"
     promoter        complement(4500..4518)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4539..4555)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4563..4579)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4587..4617)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4632..4653)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4941..5529)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"