LOCUS       V004449                 5702 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004449
VERSION     V004449
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Turnage MA, Muangsan N, Peele CG, Robertson D.
  TITLE     Geminivirus-based vectors for gene silencing in Arabidopsis
  JOURNAL   Plant J. 30 (1), 107-114 (2002)
   PUBMED   11967097
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Turnage MA, Muangsan N, Peele CG, Robertson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2003) Botany, North Carolina State University, Box
            7612, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 5702)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5702)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
            date: "2002"; volume: "30"; issue: "1"; pages: "107-114"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-APR-2003) Botany, North Carolina State University, Box 7612,
            Raleigh, NC 27695, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..858)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(859..963)
                     /label="AmpR promoter"
     rep_origin      complement(989..1444)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1586..1602
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1612..1630
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2065..3111
                     /note="Replication-associated protein from Cabbage leaf
                     curl virus (isolate Jamaica). Accession#: Q96704"
                     /label="Replication-associated protein"
     CDS             3056..3442
                     /note="Transcriptional activator protein from Cabbage leaf
                     curl virus (isolate Jamaica). Accession#: Q96703"
                     /label="Transcriptional activator protein"
     misc_feature    3652..4024
                     /note="derived from GenBank Accession Number AY140022; 360
                     bp of Arabidopsis thaliana ChlI replaces Cabbage leaf curl
                     virus coat protein"
     promoter        complement(4500..4518)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4539..4555)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4563..4579)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4587..4617)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4632..4653)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4941..5529)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
       61 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
      301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
      361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
      421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
      481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
      541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
      661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      961 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     1021 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     1081 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     1141 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     1201 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     1261 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     1321 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     1381 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     1441 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     1501 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     1561 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     1621 tcactatagg gcgaattgga acccaagcag cggccttatc aaatttatgg atcatacctg
     1681 cacgaggtga atagttagca ttgcgggaca ctttagaggt ccccgccata gaacgccacg
     1741 gggcatcccg cttaggcatt tcgaagtaaa gcataaattg aaatcacatg tctttatata
     1801 ggcccacagc gcactcagtt agccttaata gctaaccttg tcaggcggca tgtgattggt
     1861 tagatttcat ttaatgcgtg gtcccacgca agacaccaaa aaaatcgcgg ccatccggta
     1921 atattattgc ggatggccgc ttttgcccct ggtgttcccc agaggctttt agtatatatt
     1981 ggacaccaag acaccaaaaa caataggttt tagagagagc tctcctggcg tcctcccctg
     2041 gagtcctaaa tattatttac aaaaatgcca cgaaacccta aatcgtttcg tttagcagcc
     2101 cgaaatatat tcttaactca cctaaggtgc gacataccca aagatgaagc tcttcagatg
     2161 cttcaaaccc tgtcgtggtc agtcgtcaaa cccacataca tcagagtcgc aagagaggaa
     2221 cattcagacg gttccccatt tacacactgt ctcatccaac tatcaggaaa gtcgaacatc
     2281 aaggatgcta gatttttcga catcactcac cccagaaggt ctgccaattt tcacccaaac
     2341 attcaggcag ccaaagacac caatgccgtc aagaattaca tcaccaaaga tggtgattat
     2401 tgtgaatccg ggcagtacaa ggtgtctggg ggtacaaagg caaataaaga cgacgtctac
     2461 cacaacgccg tcaatgcggg atgtgtggaa gagcgtctcg caattataag ggctggagtt
     2521 ccaaagacgt tcattgttag ttatcataat gttagagcta acatagagcg actctttact
     2581 aaggctccgg aaccatgggc tcctccgttt caactctcct cctttactaa cgtcccggac
     2641 gagatgagtt catgggcaga tgactatttt ggtcggagtg ccgctgcgcg ggcggaaaga
     2701 cctattagta tcatagttga aggtgattca cgaaccggca agaccatgtg ggcgcgtgct
     2761 ttaggaccac ataattattt gagtgggcac ctcgacttta attcaaaggt cttttcaaat
     2821 aatgcggagt ataacgtcat tgatgacata gctccgcatt atctaaagct aaagcactgg
     2881 aaagagctta ttggggccca aagggactgg caatcaaact gtaagtacgg caagccagtt
     2941 caaattaaag gtggcatacc ctcaatcgtg ctgtgcaatc caggagaggg gagcagttat
     3001 ataagtttcc tcaacaaaga ggaaaatgca tcactaagag cgtggactac caaaaatgca
     3061 aaattcatca ctcttgaagc ccccctctat caaagcacag cacaagattg ctaaaaggag
     3121 agctgtacgc cgaagacgca ttgatttgaa ctgcgggtgt tctatcttcc tccacatcaa
     3181 ttgcgcagat aatggattca cgcaccgggg agagcatcac tgtagctcag gcagagaatt
     3241 ccgtttttat ctgggaggtt ccaaatcccc tatatttcag gatacaacgc gtagaggacc
     3301 cgttgtacac cagaaccagg atctaccaca tccaagtccg gttcaaccac aacctacgga
     3361 aagcattgga tctccgcaaa gcctacttca acttccaagt ctggacgact tcgatgagag
     3421 cttctgggcc gacatattta agtagattca aatgccttgt aatgtctcat ttagataacc
     3481 taggtgttat tggtattaat catgtaatta gagctgttcg tttcgcaacg gacagatcct
     3541 atgtaactca tgttcacgag aatcatgtaa taaatttcaa aatttattaa tttgttatcg
     3601 aatcgtaaaa atagatccga atttttaatg tcgcatacac aggattaggc tagcgagctc
     3661 agatctttag ttgatctgtt acctgagatt aacgtagttg caggtgaccc gtataactcg
     3721 gatcctatag atcctgagtt tatgggtgtt gaagtaagag agagagttga gaaaggagag
     3781 caagttcctg ttattgctac taagattaac atggttgatc ttcctttggg tgcaacagaa
     3841 gatagagttt gtggaaccat agatatcgaa aaggctttga cagaaggtgt aaaagccttt
     3901 gagcctggtt tgttggctaa agctaataga gggattcttt atgttgatga agttaatctc
     3961 ttggatgatc atttggttga tgttcttttg gattcagctg cttctggttg gaatacggtt
     4021 tctagattaa ttaaggtacc gctagccgct taggcatttc gaagtaaagc ataaattgaa
     4081 atcacatgtc tttatatagg cccacagcgc actcagttag ccttaatagc taaccttgtc
     4141 aggcggcatg tgattggtta gatttcattt aatgcgtggt cccacgcaag acaccaaaaa
     4201 aatcgcggcc atccggtaat attattgcgg atggccgctt ttgcccctgg tgttccccag
     4261 aggcttttag tatatattgg acaccaagac accaaaaaca ataggtttta gagagagctc
     4321 tcctggcgtc ctcccctgga gtcctaaata ttatttacaa aaatgccacg aaaccctaaa
     4381 tcgtttcgtt tagcagcccg aaatatattc ttaactcacc taaggtgcga catacccaaa
     4441 gatgaagcgg gggatccact agagcggccg ccaccgcggt ggagctccag cttttgttcc
     4501 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     4561 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     4621 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     4681 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     4741 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     4801 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     4861 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4921 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4981 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5041 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5101 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5161 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5221 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5281 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5341 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     5401 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5461 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5521 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5581 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5641 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5701 ta
//
