pMT2-PL vector (V004451)

LOCUS       V004451                 5908 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004451
VERSION     V004451
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Bair CR, Kotha Lakshmi Narayan P, Kajon AE.
  TITLE     The Adenovirus Tripartite Leader Sequence Enables Efficient Ectopic
            Expression of Human Adenovirus E3 CR1 Transcripts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Bair CR, Kotha Lakshmi Narayan P, Lamson DM, Kajon AE.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2016) Infectous Disease Program, Lovelace
            Respiratory Research Institute, 2425 Ridgecrest Drive SE,
            Albuquerque, NM 87108, USA
REFERENCE   3  (bases 1 to 5908)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5908)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Geneious v. 8.1.7
            Sequencing Technology :: Illumina; Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-AUG-2016) Infectous Disease Program, Lovelace Respiratory
            Research Institute, 2425 Ridgecrest Drive SE, Albuquerque, NM 87108,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..65
                     /label="multiple cloning sites"
                     /note="multiple cloning sites"
     polyA_signal    95..229
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(630..1718)
                     /gene="ADA"
                     /label="Adenosine deaminase"
                     /note="Adenosine deaminase from Homo sapiens. Accession#:
                     P00813"
     promoter        complement(1799..2115)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    2138..2371
                     /label="VARNA"
                     /note="VARNA"
     rep_origin      complement(2623..3211)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3385..4242)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4243..4347)
                     /label="AmpR promoter"
     primer_bind     4821..4837
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(4847..5176)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     regulatory      5204..5504
                     /label="human adenovirus type 2 major late promoter"
                     /note="human adenovirus type 2 major late promoter"
                     /regulatory_class="promoter"
     misc_feature    5505..5636
                     /note="leader 2 and leader 3 from the human adenovirus type
                     2 tripartite leader sequence"
     intron          5645..5874
                     /label="chimeric intron"
                     /note="chimera between introns from adenovirus and
                     immunoglobulin heavy chain genes"